Interaction between Microbes and Host in Sow Vaginas in Early Pregnancy
ABSTRACT Extensive research has explored the causes of embryo losses during early pregnancy by analyzing interaction mechanisms in sows’ uterus, ignoring the importance of the lower reproductive tract in pregnancy development regulation. Despite recent progress in understanding the diversity of vagi...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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American Society for Microbiology
2023-04-01
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| Series: | mSystems |
| Subjects: | |
| Online Access: | https://journals.asm.org/doi/10.1128/msystems.01192-22 |
| _version_ | 1797838311267500032 |
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| author | Xupeng Zang Wenjing Wang Shengchen Gu Ting Gu Huaqiang Yang Enqin Zheng Zheng Xu Sixiu Huang Zicong Li Gengyuan Cai Linjun Hong Zhenfang Wu |
| author_facet | Xupeng Zang Wenjing Wang Shengchen Gu Ting Gu Huaqiang Yang Enqin Zheng Zheng Xu Sixiu Huang Zicong Li Gengyuan Cai Linjun Hong Zhenfang Wu |
| author_sort | Xupeng Zang |
| collection | DOAJ |
| description | ABSTRACT Extensive research has explored the causes of embryo losses during early pregnancy by analyzing interaction mechanisms in sows’ uterus, ignoring the importance of the lower reproductive tract in pregnancy development regulation. Despite recent progress in understanding the diversity of vaginal microbes under different physiological states, the dynamic of sows’ vaginal microbiotas during pregnancy and the interaction between vaginal microbes and the host are poorly understood. Here, we performed a comprehensive analysis of sows’ vaginal microbial communities in early pregnancy coupled with overall patterns of vaginal mucosal epithelium gene expression. The vaginal microbiota was analyzed by 16s rRNA or metagenome sequencing, and the vaginal mucosal epithelium transcriptome was analyzed by RNA sequencing, followed by integration of the data layers. We found that the sows’ vaginal microbiotas in early pregnancy develop dynamically, and there is a homeostasis balance of Firmicutes and Proteobacteria. Subsequently, we identified two pregnancy-specific communities, which play diverse roles. The microbes in the vagina stimulate the epithelial cells, while vaginal epithelium changes its structure and functions in response to stimulation. These changes produce specific inflammation responses to promote pregnancy development. Our findings demonstrate the interaction between microbes and host in the sow vagina in early pregnancy to promote pregnancy development, meanwhile providing a reference data set for the study of targeted therapies of microbial homeostasis dysregulation in the female reproductive tract. IMPORTANCE This work sheds light on the dynamics of the sow vaginal microbiotas in early pregnancy and its roles in pregnancy development. Furthermore, this study provides insight into the functional mechanisms of reproductive tract microbes by outlining vaginal microbe-host interactions, which might identify new research and intervention targets for improving pregnancy development by modulating lower reproductive tract microbiota. |
| first_indexed | 2024-04-09T15:39:54Z |
| format | Article |
| id | doaj.art-7e72c102f7874889bbd695f33b4d0f5b |
| institution | Directory Open Access Journal |
| issn | 2379-5077 |
| language | English |
| last_indexed | 2024-04-09T15:39:54Z |
| publishDate | 2023-04-01 |
| publisher | American Society for Microbiology |
| record_format | Article |
| series | mSystems |
| spelling | doaj.art-7e72c102f7874889bbd695f33b4d0f5b2023-04-27T13:02:44ZengAmerican Society for MicrobiologymSystems2379-50772023-04-018210.1128/msystems.01192-22Interaction between Microbes and Host in Sow Vaginas in Early PregnancyXupeng Zang0Wenjing Wang1Shengchen Gu2Ting Gu3Huaqiang Yang4Enqin Zheng5Zheng Xu6Sixiu Huang7Zicong Li8Gengyuan Cai9Linjun Hong10Zhenfang Wu11National Engineering Research Center for Breeding Swine Industry, College of Animal Science, South China Agricultural University, Guangzhou, ChinaNational Engineering Research Center for Breeding Swine Industry, College of Animal Science, South China Agricultural University, Guangzhou, ChinaNational Engineering Research Center for Breeding Swine Industry, College of Animal Science, South China Agricultural University, Guangzhou, ChinaNational Engineering Research Center for Breeding Swine Industry, College of Animal Science, South China Agricultural University, Guangzhou, ChinaNational Engineering Research Center for Breeding Swine Industry, College of Animal Science, South China Agricultural University, Guangzhou, ChinaNational Engineering Research Center for Breeding Swine Industry, College of Animal Science, South China Agricultural University, Guangzhou, ChinaNational Engineering Research Center for Breeding Swine Industry, College of Animal Science, South China Agricultural University, Guangzhou, ChinaNational Engineering Research Center for Breeding Swine Industry, College of Animal Science, South China Agricultural University, Guangzhou, ChinaNational Engineering Research Center for Breeding Swine Industry, College of Animal Science, South China Agricultural University, Guangzhou, ChinaNational Engineering Research Center for Breeding Swine Industry, College of Animal Science, South China Agricultural University, Guangzhou, ChinaNational Engineering Research Center for Breeding Swine Industry, College of Animal Science, South China Agricultural University, Guangzhou, ChinaNational Engineering Research Center for Breeding Swine Industry, College of Animal Science, South China Agricultural University, Guangzhou, ChinaABSTRACT Extensive research has explored the causes of embryo losses during early pregnancy by analyzing interaction mechanisms in sows’ uterus, ignoring the importance of the lower reproductive tract in pregnancy development regulation. Despite recent progress in understanding the diversity of vaginal microbes under different physiological states, the dynamic of sows’ vaginal microbiotas during pregnancy and the interaction between vaginal microbes and the host are poorly understood. Here, we performed a comprehensive analysis of sows’ vaginal microbial communities in early pregnancy coupled with overall patterns of vaginal mucosal epithelium gene expression. The vaginal microbiota was analyzed by 16s rRNA or metagenome sequencing, and the vaginal mucosal epithelium transcriptome was analyzed by RNA sequencing, followed by integration of the data layers. We found that the sows’ vaginal microbiotas in early pregnancy develop dynamically, and there is a homeostasis balance of Firmicutes and Proteobacteria. Subsequently, we identified two pregnancy-specific communities, which play diverse roles. The microbes in the vagina stimulate the epithelial cells, while vaginal epithelium changes its structure and functions in response to stimulation. These changes produce specific inflammation responses to promote pregnancy development. Our findings demonstrate the interaction between microbes and host in the sow vagina in early pregnancy to promote pregnancy development, meanwhile providing a reference data set for the study of targeted therapies of microbial homeostasis dysregulation in the female reproductive tract. IMPORTANCE This work sheds light on the dynamics of the sow vaginal microbiotas in early pregnancy and its roles in pregnancy development. Furthermore, this study provides insight into the functional mechanisms of reproductive tract microbes by outlining vaginal microbe-host interactions, which might identify new research and intervention targets for improving pregnancy development by modulating lower reproductive tract microbiota.https://journals.asm.org/doi/10.1128/msystems.01192-22developing microbiotaearly pregnancypigvaginal microbiota |
| spellingShingle | Xupeng Zang Wenjing Wang Shengchen Gu Ting Gu Huaqiang Yang Enqin Zheng Zheng Xu Sixiu Huang Zicong Li Gengyuan Cai Linjun Hong Zhenfang Wu Interaction between Microbes and Host in Sow Vaginas in Early Pregnancy mSystems developing microbiota early pregnancy pig vaginal microbiota |
| title | Interaction between Microbes and Host in Sow Vaginas in Early Pregnancy |
| title_full | Interaction between Microbes and Host in Sow Vaginas in Early Pregnancy |
| title_fullStr | Interaction between Microbes and Host in Sow Vaginas in Early Pregnancy |
| title_full_unstemmed | Interaction between Microbes and Host in Sow Vaginas in Early Pregnancy |
| title_short | Interaction between Microbes and Host in Sow Vaginas in Early Pregnancy |
| title_sort | interaction between microbes and host in sow vaginas in early pregnancy |
| topic | developing microbiota early pregnancy pig vaginal microbiota |
| url | https://journals.asm.org/doi/10.1128/msystems.01192-22 |
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