In Vitro Inhibition of Biofilm Formation by Staphylococcus Aureus Under the Action of Selected Plant Extracts
In our study we investigated the ability of selected plant extracts to inhibit the formation of biofilms produced by Staphylococcus aureus. In the first phase, we focused on the optimisation of conditions for the correct method of an approach. For optimisation, we standardized the culture media and...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Sciendo
2019-03-01
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| Series: | Folia Veterinaria |
| Subjects: | |
| Online Access: | https://doi.org/10.2478/fv-2019-0007 |
| _version_ | 1819147552009748480 |
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| author | Piegerová A. Koščová J. Schusterová P. Nemcová R. Kryvtsova M. |
| author_facet | Piegerová A. Koščová J. Schusterová P. Nemcová R. Kryvtsova M. |
| author_sort | Piegerová A. |
| collection | DOAJ |
| description | In our study we investigated the ability of selected plant extracts to inhibit the formation of biofilms produced by Staphylococcus aureus. In the first phase, we focused on the optimisation of conditions for the correct method of an approach. For optimisation, we standardized the culture media and the bacterial culture in order to obtain interpretable results. The TSB (Tryptone Soya Broth) medium was used for the preparation of an inoculum from the bacterial suspension. For the in vitro tests of antibiofilm activity against the species Staphylococcus aureus CCM 3953, we used propylene glycol (PG) plant extracts from sage and rosemary, prepared in three different concentrations of 0.01 %, 0.05 % and 0.1 %. The tests were implemented in microtitre plates using crystal violet dye at 0.1 % concentration for visualization of the intensity of a biofilm. The results were obtained, by spectrophotometric measurements at a wavelength of 550 nm. Both rosemary and sage plant extracts had a significant effect on the formation of a biofilm by S. aureus. The antibiofilm activity was concentration-dependent as the formation of biofilm was reduced more effectively with increasing concentration of the extracts. The best antibiofilm activity was observed with 0.1 % rosemary extract resulting in 94 % inhibition of the biofilm formation. |
| first_indexed | 2024-12-22T13:31:38Z |
| format | Article |
| id | doaj.art-7e73ca91c4e541698725e92beb18b28f |
| institution | Directory Open Access Journal |
| issn | 2453-7837 |
| language | English |
| last_indexed | 2024-12-22T13:31:38Z |
| publishDate | 2019-03-01 |
| publisher | Sciendo |
| record_format | Article |
| series | Folia Veterinaria |
| spelling | doaj.art-7e73ca91c4e541698725e92beb18b28f2022-12-21T18:24:10ZengSciendoFolia Veterinaria2453-78372019-03-01631485310.2478/fv-2019-0007fv-2019-0007In Vitro Inhibition of Biofilm Formation by Staphylococcus Aureus Under the Action of Selected Plant ExtractsPiegerová A.0Koščová J.1Schusterová P.2Nemcová R.3Kryvtsova M.4Department of Microbiology and ImmunologyUniversity of Veterinary Medicine and Pharmacy, Komenského 73, 041 81KošiceSlovakiaDepartment of Microbiology and ImmunologyUniversity of Veterinary Medicine and Pharmacy, Komenského 73, 041 81KošiceSlovakiaDepartment of Microbiology and ImmunologyUniversity of Veterinary Medicine and Pharmacy, Komenského 73, 041 81KošiceSlovakiaDepartment of Microbiology and ImmunologyUniversity of Veterinary Medicine and Pharmacy, Komenského 73, 041 81KošiceSlovakiaDepartment of Genetics, Plant Physiology and Microbiology, Biological FacultyUzhhorod National University, UzhhorodUkraineIn our study we investigated the ability of selected plant extracts to inhibit the formation of biofilms produced by Staphylococcus aureus. In the first phase, we focused on the optimisation of conditions for the correct method of an approach. For optimisation, we standardized the culture media and the bacterial culture in order to obtain interpretable results. The TSB (Tryptone Soya Broth) medium was used for the preparation of an inoculum from the bacterial suspension. For the in vitro tests of antibiofilm activity against the species Staphylococcus aureus CCM 3953, we used propylene glycol (PG) plant extracts from sage and rosemary, prepared in three different concentrations of 0.01 %, 0.05 % and 0.1 %. The tests were implemented in microtitre plates using crystal violet dye at 0.1 % concentration for visualization of the intensity of a biofilm. The results were obtained, by spectrophotometric measurements at a wavelength of 550 nm. Both rosemary and sage plant extracts had a significant effect on the formation of a biofilm by S. aureus. The antibiofilm activity was concentration-dependent as the formation of biofilm was reduced more effectively with increasing concentration of the extracts. The best antibiofilm activity was observed with 0.1 % rosemary extract resulting in 94 % inhibition of the biofilm formation.https://doi.org/10.2478/fv-2019-0007biofilminhibitionplant extractsstaphylococcus aureus |
| spellingShingle | Piegerová A. Koščová J. Schusterová P. Nemcová R. Kryvtsova M. In Vitro Inhibition of Biofilm Formation by Staphylococcus Aureus Under the Action of Selected Plant Extracts Folia Veterinaria biofilm inhibition plant extracts staphylococcus aureus |
| title | In Vitro Inhibition of Biofilm Formation by Staphylococcus Aureus Under the Action of Selected Plant Extracts |
| title_full | In Vitro Inhibition of Biofilm Formation by Staphylococcus Aureus Under the Action of Selected Plant Extracts |
| title_fullStr | In Vitro Inhibition of Biofilm Formation by Staphylococcus Aureus Under the Action of Selected Plant Extracts |
| title_full_unstemmed | In Vitro Inhibition of Biofilm Formation by Staphylococcus Aureus Under the Action of Selected Plant Extracts |
| title_short | In Vitro Inhibition of Biofilm Formation by Staphylococcus Aureus Under the Action of Selected Plant Extracts |
| title_sort | in vitro inhibition of biofilm formation by staphylococcus aureus under the action of selected plant extracts |
| topic | biofilm inhibition plant extracts staphylococcus aureus |
| url | https://doi.org/10.2478/fv-2019-0007 |
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