Serum LDL- and HDL-cholesterol determined by ultracentrifugation and HPLC
Simple and precise methods for LDL-cholesterol (LDL-C) and HDL-cholesterol (HDL-C) measurements are essential for assessment of cardiovascular disease (CVD) risks and for lipid and lipoprotein studies. We report here an ultracentrifugation (UC) and HPLC method that requires substantially less specim...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2011-02-01
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| Series: | Journal of Lipid Research |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520405346 |
| _version_ | 1830486955460132864 |
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| author | Jun Dong Hanbang Guo Ruiyue Yang Hongxia Li Shu Wang Jiangtao Zhang Wenxiang Chen |
| author_facet | Jun Dong Hanbang Guo Ruiyue Yang Hongxia Li Shu Wang Jiangtao Zhang Wenxiang Chen |
| author_sort | Jun Dong |
| collection | DOAJ |
| description | Simple and precise methods for LDL-cholesterol (LDL-C) and HDL-cholesterol (HDL-C) measurements are essential for assessment of cardiovascular disease (CVD) risks and for lipid and lipoprotein studies. We report here an ultracentrifugation (UC) and HPLC method that requires substantially less specimen volume and provides the necessary reliability and throughput required by large-volume, high-quality research and clinical studies. 2-Mercaptoethanol (ME) was used to dissociate serum lipoprotein [a] (Lp[a]) into apolipoprotein [a] and Lp[a] remnant (Lp[a−]) and eliminated the contamination of Lp[a] in HDL separated by UC. Serum aliquots were centrifuged at a density of 1.006 kg/l for the separation of HDL plus LDL, and in the presence of ME at a density of 1.063 kg/l for the separation of HDL. Cholesterol concentrations of the bottom fractions were analyzed by HPLC. LDL-C and HDL-C determined using this method were equivalent to those with β-quantification and the designated comparison method of the Centers for Disease Control. The total coefficient of variations for LDL-C and HDL-C were 0.65–1.12% and 0.96–2.07%, respectively. This method requires a small amount of specimen and is easy to operate. This method may be used in research or in clinical laboratories where precise and specific lipoprotein cholesterol analysis is needed. |
| first_indexed | 2024-12-21T18:53:07Z |
| format | Article |
| id | doaj.art-7e7682d0e4be428ab52a6d3fa15ef771 |
| institution | Directory Open Access Journal |
| issn | 0022-2275 |
| language | English |
| last_indexed | 2024-12-21T18:53:07Z |
| publishDate | 2011-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Lipid Research |
| spelling | doaj.art-7e7682d0e4be428ab52a6d3fa15ef7712022-12-21T18:53:42ZengElsevierJournal of Lipid Research0022-22752011-02-01522383388Serum LDL- and HDL-cholesterol determined by ultracentrifugation and HPLCJun Dong0Hanbang Guo1Ruiyue Yang2Hongxia Li3Shu Wang4Jiangtao Zhang5Wenxiang Chen6Institute of Geriatrics Beijing Hospital, Beijing 100730, ChinaInstitute of Geriatrics Beijing Hospital, Beijing 100730, ChinaInstitute of Geriatrics Beijing Hospital, Beijing 100730, ChinaInstitute of Geriatrics Beijing Hospital, Beijing 100730, ChinaInstitute of Geriatrics Beijing Hospital, Beijing 100730, ChinaNational Center for Clinical Laboratories, Beijing Hospital, Beijing 100730, ChinaTo whom correspondence should be addressed.; Institute of Geriatrics Beijing Hospital, Beijing 100730, China; National Center for Clinical Laboratories, Beijing Hospital, Beijing 100730, ChinaSimple and precise methods for LDL-cholesterol (LDL-C) and HDL-cholesterol (HDL-C) measurements are essential for assessment of cardiovascular disease (CVD) risks and for lipid and lipoprotein studies. We report here an ultracentrifugation (UC) and HPLC method that requires substantially less specimen volume and provides the necessary reliability and throughput required by large-volume, high-quality research and clinical studies. 2-Mercaptoethanol (ME) was used to dissociate serum lipoprotein [a] (Lp[a]) into apolipoprotein [a] and Lp[a] remnant (Lp[a−]) and eliminated the contamination of Lp[a] in HDL separated by UC. Serum aliquots were centrifuged at a density of 1.006 kg/l for the separation of HDL plus LDL, and in the presence of ME at a density of 1.063 kg/l for the separation of HDL. Cholesterol concentrations of the bottom fractions were analyzed by HPLC. LDL-C and HDL-C determined using this method were equivalent to those with β-quantification and the designated comparison method of the Centers for Disease Control. The total coefficient of variations for LDL-C and HDL-C were 0.65–1.12% and 0.96–2.07%, respectively. This method requires a small amount of specimen and is easy to operate. This method may be used in research or in clinical laboratories where precise and specific lipoprotein cholesterol analysis is needed.http://www.sciencedirect.com/science/article/pii/S0022227520405346low density lipoprotein-cholesterolhigh density lipoprotein-cholesterolhigh-performance liquid chromatography |
| spellingShingle | Jun Dong Hanbang Guo Ruiyue Yang Hongxia Li Shu Wang Jiangtao Zhang Wenxiang Chen Serum LDL- and HDL-cholesterol determined by ultracentrifugation and HPLC Journal of Lipid Research low density lipoprotein-cholesterol high density lipoprotein-cholesterol high-performance liquid chromatography |
| title | Serum LDL- and HDL-cholesterol determined by ultracentrifugation and HPLC |
| title_full | Serum LDL- and HDL-cholesterol determined by ultracentrifugation and HPLC |
| title_fullStr | Serum LDL- and HDL-cholesterol determined by ultracentrifugation and HPLC |
| title_full_unstemmed | Serum LDL- and HDL-cholesterol determined by ultracentrifugation and HPLC |
| title_short | Serum LDL- and HDL-cholesterol determined by ultracentrifugation and HPLC |
| title_sort | serum ldl and hdl cholesterol determined by ultracentrifugation and hplc |
| topic | low density lipoprotein-cholesterol high density lipoprotein-cholesterol high-performance liquid chromatography |
| url | http://www.sciencedirect.com/science/article/pii/S0022227520405346 |
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