A New Method for Enantiomeric Determination of 3,4-Methylenedioxymethamphetamine and p-Methoxymethamphetamine in Human Urine
The abuse of paramethoxymethamphetamine (PMMA) and 3,4-methylenedioxymethamphetamine (MDMA) among young people is increasingly serious and has become a public health problem. Since enantiomers of MDMA and PMMA are metabolized at different rates in the body and exhibit different neurotoxicity in tiss...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-01-01
|
| Series: | Chemosensors |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2227-9040/10/2/50 |
| _version_ | 1797481769965977600 |
|---|---|
| author | Wei-Jay Chen Ren-Jang Wu Hsueh-Hui Yang Ahai-Chang Lua Yin-Jeh Tzeng Shun-Hsing Tuan Liang Chen Tsong-Yung Chou |
| author_facet | Wei-Jay Chen Ren-Jang Wu Hsueh-Hui Yang Ahai-Chang Lua Yin-Jeh Tzeng Shun-Hsing Tuan Liang Chen Tsong-Yung Chou |
| author_sort | Wei-Jay Chen |
| collection | DOAJ |
| description | The abuse of paramethoxymethamphetamine (PMMA) and 3,4-methylenedioxymethamphetamine (MDMA) among young people is increasingly serious and has become a public health problem. Since enantiomers of MDMA and PMMA are metabolized at different rates in the body and exhibit different neurotoxicity in tissues, we have developed a simple method for simultaneous enantiomeric determination of PMMA and MDMA, using parallel dual capillary immunoaffinity columns coupled with tandem mass spectrometry. Linear calibration curves were obtained in concentration ranges of 100–1000 ng/mL, with a limit of quantitation of <22 ng/mL. Good interday accuracy and precision were achieved with this method. Besides filtering the urine sample through a 0.45 μm MILLIPORE membrane, no other sample pretreatment was needed, and no toxic organic solvent was used. It is a rapid, environmentally friendly safe method, and could be applied for routine enantiomeric analysis of PMMA and MDMA in the pharmaceutical industry, forensic science, and environmental analysis. |
| first_indexed | 2024-03-09T22:19:17Z |
| format | Article |
| id | doaj.art-7e7cbe19cec1487384edf99ec3342145 |
| institution | Directory Open Access Journal |
| issn | 2227-9040 |
| language | English |
| last_indexed | 2024-03-09T22:19:17Z |
| publishDate | 2022-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Chemosensors |
| spelling | doaj.art-7e7cbe19cec1487384edf99ec33421452023-11-23T19:17:05ZengMDPI AGChemosensors2227-90402022-01-011025010.3390/chemosensors10020050A New Method for Enantiomeric Determination of 3,4-Methylenedioxymethamphetamine and p-Methoxymethamphetamine in Human UrineWei-Jay Chen0Ren-Jang Wu1Hsueh-Hui Yang2Ahai-Chang Lua3Yin-Jeh Tzeng4Shun-Hsing Tuan5Liang Chen6Tsong-Yung Chou7Department of Laboratory Medicine and Biotechnology, Graduate Institute of Medical Biotechnology, Tzu Chi University, Hualien 970, TaiwanDepartment of Applied Chemistry, Providence University, Taichung 43301, TaiwanDepartment of Medical Research, Hualien Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, Hualien 970, TaiwanCenter for Drug Analysis, Tzu Chi University, Hualien 970, TaiwanDepartment of Molecular Biology and Human Genetics, Tzu Chi University, Hualien 970, TaiwanDepartment of Medicine, Tzu Chi University, Hualien 970, TaiwanDepartment of Medicine, Tzu Chi University, Hualien 970, TaiwanDepartment of Laboratory Medicine and Biotechnology, Graduate Institute of Medical Biotechnology, Tzu Chi University, Hualien 970, TaiwanThe abuse of paramethoxymethamphetamine (PMMA) and 3,4-methylenedioxymethamphetamine (MDMA) among young people is increasingly serious and has become a public health problem. Since enantiomers of MDMA and PMMA are metabolized at different rates in the body and exhibit different neurotoxicity in tissues, we have developed a simple method for simultaneous enantiomeric determination of PMMA and MDMA, using parallel dual capillary immunoaffinity columns coupled with tandem mass spectrometry. Linear calibration curves were obtained in concentration ranges of 100–1000 ng/mL, with a limit of quantitation of <22 ng/mL. Good interday accuracy and precision were achieved with this method. Besides filtering the urine sample through a 0.45 μm MILLIPORE membrane, no other sample pretreatment was needed, and no toxic organic solvent was used. It is a rapid, environmentally friendly safe method, and could be applied for routine enantiomeric analysis of PMMA and MDMA in the pharmaceutical industry, forensic science, and environmental analysis.https://www.mdpi.com/2227-9040/10/2/50capillary immunoaffinity columnenantiomeric determinationLC-MS/MS3,4-methylenedioxymethamphetamineparamethoxymethamphetamine |
| spellingShingle | Wei-Jay Chen Ren-Jang Wu Hsueh-Hui Yang Ahai-Chang Lua Yin-Jeh Tzeng Shun-Hsing Tuan Liang Chen Tsong-Yung Chou A New Method for Enantiomeric Determination of 3,4-Methylenedioxymethamphetamine and p-Methoxymethamphetamine in Human Urine Chemosensors capillary immunoaffinity column enantiomeric determination LC-MS/MS 3,4-methylenedioxymethamphetamine paramethoxymethamphetamine |
| title | A New Method for Enantiomeric Determination of 3,4-Methylenedioxymethamphetamine and p-Methoxymethamphetamine in Human Urine |
| title_full | A New Method for Enantiomeric Determination of 3,4-Methylenedioxymethamphetamine and p-Methoxymethamphetamine in Human Urine |
| title_fullStr | A New Method for Enantiomeric Determination of 3,4-Methylenedioxymethamphetamine and p-Methoxymethamphetamine in Human Urine |
| title_full_unstemmed | A New Method for Enantiomeric Determination of 3,4-Methylenedioxymethamphetamine and p-Methoxymethamphetamine in Human Urine |
| title_short | A New Method for Enantiomeric Determination of 3,4-Methylenedioxymethamphetamine and p-Methoxymethamphetamine in Human Urine |
| title_sort | new method for enantiomeric determination of 3 4 methylenedioxymethamphetamine and p methoxymethamphetamine in human urine |
| topic | capillary immunoaffinity column enantiomeric determination LC-MS/MS 3,4-methylenedioxymethamphetamine paramethoxymethamphetamine |
| url | https://www.mdpi.com/2227-9040/10/2/50 |
| work_keys_str_mv | AT weijaychen anewmethodforenantiomericdeterminationof34methylenedioxymethamphetamineandpmethoxymethamphetamineinhumanurine AT renjangwu anewmethodforenantiomericdeterminationof34methylenedioxymethamphetamineandpmethoxymethamphetamineinhumanurine AT hsuehhuiyang anewmethodforenantiomericdeterminationof34methylenedioxymethamphetamineandpmethoxymethamphetamineinhumanurine AT ahaichanglua anewmethodforenantiomericdeterminationof34methylenedioxymethamphetamineandpmethoxymethamphetamineinhumanurine AT yinjehtzeng anewmethodforenantiomericdeterminationof34methylenedioxymethamphetamineandpmethoxymethamphetamineinhumanurine AT shunhsingtuan anewmethodforenantiomericdeterminationof34methylenedioxymethamphetamineandpmethoxymethamphetamineinhumanurine AT liangchen anewmethodforenantiomericdeterminationof34methylenedioxymethamphetamineandpmethoxymethamphetamineinhumanurine AT tsongyungchou anewmethodforenantiomericdeterminationof34methylenedioxymethamphetamineandpmethoxymethamphetamineinhumanurine AT weijaychen newmethodforenantiomericdeterminationof34methylenedioxymethamphetamineandpmethoxymethamphetamineinhumanurine AT renjangwu newmethodforenantiomericdeterminationof34methylenedioxymethamphetamineandpmethoxymethamphetamineinhumanurine AT hsuehhuiyang newmethodforenantiomericdeterminationof34methylenedioxymethamphetamineandpmethoxymethamphetamineinhumanurine AT ahaichanglua newmethodforenantiomericdeterminationof34methylenedioxymethamphetamineandpmethoxymethamphetamineinhumanurine AT yinjehtzeng newmethodforenantiomericdeterminationof34methylenedioxymethamphetamineandpmethoxymethamphetamineinhumanurine AT shunhsingtuan newmethodforenantiomericdeterminationof34methylenedioxymethamphetamineandpmethoxymethamphetamineinhumanurine AT liangchen newmethodforenantiomericdeterminationof34methylenedioxymethamphetamineandpmethoxymethamphetamineinhumanurine AT tsongyungchou newmethodforenantiomericdeterminationof34methylenedioxymethamphetamineandpmethoxymethamphetamineinhumanurine |