Production and Quality Control of Technetium-99m Radiolabeled Monoclonal Antibody
AbstractBackground and purpose: Binding a monoclonal antibody to tumor associated antigens is an effective method for cancer therapy because these agents can specifically target malignant cells. In fact, monoclonal antibodies are effective agents for diagnosis, grading and treatment of different kin...
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| Format: | Article |
| Language: | English |
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Mazandaran University of Medical Sciences
2007-01-01
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| Series: | Journal of Mazandaran University of Medical Sciences |
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| Online Access: | http://www.mazums.ac.ir/index.php?digital_library&do=downloadPdf&sid=526 |
| _version_ | 1818833224346894336 |
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| author | M.H. Babaei, Ph.D. Sh. Montaser kuhsari, Ph.D R. Najafi, Ph.D F. Joharidaha, Ph.D. M. Shafiei, M.Sc. P. Behradkia, M.Sc N. Zarrabi ahrabi, M.Sc. |
| author_facet | M.H. Babaei, Ph.D. Sh. Montaser kuhsari, Ph.D R. Najafi, Ph.D F. Joharidaha, Ph.D. M. Shafiei, M.Sc. P. Behradkia, M.Sc N. Zarrabi ahrabi, M.Sc. |
| author_sort | M.H. Babaei, Ph.D. |
| collection | DOAJ |
| description | AbstractBackground and purpose: Binding a monoclonal antibody to tumor associated antigens is an effective method for cancer therapy because these agents can specifically target malignant cells. In fact, monoclonal antibodies are effective agents for diagnosis, grading and treatment of different kinds of cancers. In this research, a new monoclonal antibody against colon cancer cells was prepared and radiolabeling with technetium-99m evaluated.Materials and Methods: This research was done in three parts: preparation of hybridoma cell against colon cancer cell line (HT29), production of monoclonal antibody, determination of its characterizations and radiolabeling with technetium-99m.Results: mAb-D2 is an IgG1 with affinity constant of 7.2 × 109M-1 which can recognize CEA in tumor cells. Radiolabeling showed that 99mTc-HYNIC-mAb-D2 complex is stable, immunoradioactive, and has a desirable biodistribution.Conclusion: In this study, we gained a new radiopharmaceutical that may be a good candidate for radioimmunoscintigraphy. |
| first_indexed | 2024-12-19T02:15:31Z |
| format | Article |
| id | doaj.art-7e81efc36233417c9a16f480de3954f9 |
| institution | Directory Open Access Journal |
| issn | 1735-9260 1735-9279 |
| language | English |
| last_indexed | 2024-12-19T02:15:31Z |
| publishDate | 2007-01-01 |
| publisher | Mazandaran University of Medical Sciences |
| record_format | Article |
| series | Journal of Mazandaran University of Medical Sciences |
| spelling | doaj.art-7e81efc36233417c9a16f480de3954f92022-12-21T20:40:27ZengMazandaran University of Medical SciencesJournal of Mazandaran University of Medical Sciences1735-92601735-92792007-01-011760613Production and Quality Control of Technetium-99m Radiolabeled Monoclonal AntibodyM.H. Babaei, Ph.D.Sh. Montaser kuhsari, Ph.DR. Najafi, Ph.DF. Joharidaha, Ph.D.M. Shafiei, M.Sc.P. Behradkia, M.ScN. Zarrabi ahrabi, M.Sc.AbstractBackground and purpose: Binding a monoclonal antibody to tumor associated antigens is an effective method for cancer therapy because these agents can specifically target malignant cells. In fact, monoclonal antibodies are effective agents for diagnosis, grading and treatment of different kinds of cancers. In this research, a new monoclonal antibody against colon cancer cells was prepared and radiolabeling with technetium-99m evaluated.Materials and Methods: This research was done in three parts: preparation of hybridoma cell against colon cancer cell line (HT29), production of monoclonal antibody, determination of its characterizations and radiolabeling with technetium-99m.Results: mAb-D2 is an IgG1 with affinity constant of 7.2 × 109M-1 which can recognize CEA in tumor cells. Radiolabeling showed that 99mTc-HYNIC-mAb-D2 complex is stable, immunoradioactive, and has a desirable biodistribution.Conclusion: In this study, we gained a new radiopharmaceutical that may be a good candidate for radioimmunoscintigraphy.http://www.mazums.ac.ir/index.php?digital_library&do=downloadPdf&sid=526Monoclonal antibodyRadiopharmaceuticalTechnetium-99mRadioimmunoscintigraphyColon |
| spellingShingle | M.H. Babaei, Ph.D. Sh. Montaser kuhsari, Ph.D R. Najafi, Ph.D F. Joharidaha, Ph.D. M. Shafiei, M.Sc. P. Behradkia, M.Sc N. Zarrabi ahrabi, M.Sc. Production and Quality Control of Technetium-99m Radiolabeled Monoclonal Antibody Journal of Mazandaran University of Medical Sciences Monoclonal antibody Radiopharmaceutical Technetium-99m Radioimmunoscintigraphy Colon |
| title | Production and Quality Control of Technetium-99m Radiolabeled Monoclonal Antibody |
| title_full | Production and Quality Control of Technetium-99m Radiolabeled Monoclonal Antibody |
| title_fullStr | Production and Quality Control of Technetium-99m Radiolabeled Monoclonal Antibody |
| title_full_unstemmed | Production and Quality Control of Technetium-99m Radiolabeled Monoclonal Antibody |
| title_short | Production and Quality Control of Technetium-99m Radiolabeled Monoclonal Antibody |
| title_sort | production and quality control of technetium 99m radiolabeled monoclonal antibody |
| topic | Monoclonal antibody Radiopharmaceutical Technetium-99m Radioimmunoscintigraphy Colon |
| url | http://www.mazums.ac.ir/index.php?digital_library&do=downloadPdf&sid=526 |
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