Investigation of Dientamoeba fragilis Frequency in Faecal Samples of Patients with Enterobius vermicularis Infection by Polymerase Chain Reaction
Objective:Dientamoeba fragilis (D. fragilis) is a flagellated protozoan with an amoeba-like morphology, located in the gastrointestinal tract. The hypothesis was that the parasite was transported by Enterobius vermicularis (E. vermicularis) eggs. This study aimed to determine the association of D. f...
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| Format: | Article |
| Language: | English |
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Galenos Yayinevi
2021-09-01
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| Series: | Türkiye Parazitoloji Dergisi |
| Subjects: | |
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http://www.turkiyeparazitolderg.org/archives/archive-detail/article-preview/nvestigation-of-idientamoeba-fragilis-i-frequency-/48457
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| _version_ | 1828028286961188864 |
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| author | İbrahim Yıldız Sema Ertuğ Evren Tileklioğlu Erdoğan Malatyalı Özgür Güçlü Hatice Ertabaklar |
| author_facet | İbrahim Yıldız Sema Ertuğ Evren Tileklioğlu Erdoğan Malatyalı Özgür Güçlü Hatice Ertabaklar |
| author_sort | İbrahim Yıldız |
| collection | DOAJ |
| description | Objective:Dientamoeba fragilis (D. fragilis) is a flagellated protozoan with an amoeba-like morphology, located in the gastrointestinal tract. The hypothesis was that the parasite was transported by Enterobius vermicularis (E. vermicularis) eggs. This study aimed to determine the association of D. fragilis and E. vermicularis with the genotypes of the identified strain of D. fragilis. Results of trichrome staining were compared with those of polymerase chain reaction (PCR), which is widely used in the diagnosis of D. fragilis.Methods:A total of 391 samples were obtained. The stool and cellophane slide samples were sent together to the Parasitology Department Laboratory, Faculty of Medicine, Aydın Adnan Menderes University, between 1 October 2017 and 1 October 2018. Stool samples of all patients with E. vermicularis (n=74) and without E. vermicularis (n=74) infection were used. All samples were examined for the presence of D. fragilis by trichrome staining and PCR. The 18S ribosomal RNA region of D. fragilis isolates was sequenced. Demographic characteristics and clinical findings of the patients were evaluated.Results:D. fragilis was detected in 42 (28.37%) of 148 samples; 28 (66.6%) of them were detected in patients with E. vermicularis infection. The coexistence of two parasites was significant (p<0.05). All isolates sequenced were genotype 1. No significant relationship was found between the presence of parasites and clinical findings, living area and gender (p>0.05).Conclusion:D. fragilis is frequently associated with E. vermicularis, so the presence of D. fragilis should be also considered in affected patients. The use of high-sensitivity molecular methods such as PCR is important in preventing false results. |
| first_indexed | 2024-04-10T13:51:07Z |
| format | Article |
| id | doaj.art-7e85659d43bd4602b1c268e1095622d9 |
| institution | Directory Open Access Journal |
| issn | 2146-3077 2146-3077 |
| language | English |
| last_indexed | 2024-04-10T13:51:07Z |
| publishDate | 2021-09-01 |
| publisher | Galenos Yayinevi |
| record_format | Article |
| series | Türkiye Parazitoloji Dergisi |
| spelling | doaj.art-7e85659d43bd4602b1c268e1095622d92023-02-15T16:10:43ZengGalenos YayineviTürkiye Parazitoloji Dergisi2146-30772146-30772021-09-0145319520010.4274/tpd.galenos.2021.725713049054Investigation of Dientamoeba fragilis Frequency in Faecal Samples of Patients with Enterobius vermicularis Infection by Polymerase Chain Reactionİbrahim Yıldız0Sema Ertuğ1Evren Tileklioğlu2Erdoğan Malatyalı3Özgür Güçlü4Hatice Ertabaklar5 Aydın Adnan Menderes Üniversitesi Tıp Fakültesi, Parazitoloji Anabilim Dalı, Aydın, Türkiye Aydın Adnan Menderes Üniversitesi Tıp Fakültesi, Parazitoloji Anabilim Dalı, Aydın, Türkiye Aydın Adnan Menderes Üniversitesi Tıp Fakültesi, Parazitoloji Anabilim Dalı, Aydın, Türkiye Aydın Adnan Menderes Üniversitesi Tıp Fakültesi, Parazitoloji Anabilim Dalı, Aydın, Türkiye Aydın Adnan Menderes Üniversitesi Üniversitesi, Sultanhisar Meslek Yüksek Okulu, Aydın, Türkiye Aydın Adnan Menderes Üniversitesi Tıp Fakültesi, Parazitoloji Anabilim Dalı, Aydın, Türkiye Objective:Dientamoeba fragilis (D. fragilis) is a flagellated protozoan with an amoeba-like morphology, located in the gastrointestinal tract. The hypothesis was that the parasite was transported by Enterobius vermicularis (E. vermicularis) eggs. This study aimed to determine the association of D. fragilis and E. vermicularis with the genotypes of the identified strain of D. fragilis. Results of trichrome staining were compared with those of polymerase chain reaction (PCR), which is widely used in the diagnosis of D. fragilis.Methods:A total of 391 samples were obtained. The stool and cellophane slide samples were sent together to the Parasitology Department Laboratory, Faculty of Medicine, Aydın Adnan Menderes University, between 1 October 2017 and 1 October 2018. Stool samples of all patients with E. vermicularis (n=74) and without E. vermicularis (n=74) infection were used. All samples were examined for the presence of D. fragilis by trichrome staining and PCR. The 18S ribosomal RNA region of D. fragilis isolates was sequenced. Demographic characteristics and clinical findings of the patients were evaluated.Results:D. fragilis was detected in 42 (28.37%) of 148 samples; 28 (66.6%) of them were detected in patients with E. vermicularis infection. The coexistence of two parasites was significant (p<0.05). All isolates sequenced were genotype 1. No significant relationship was found between the presence of parasites and clinical findings, living area and gender (p>0.05).Conclusion:D. fragilis is frequently associated with E. vermicularis, so the presence of D. fragilis should be also considered in affected patients. The use of high-sensitivity molecular methods such as PCR is important in preventing false results. http://www.turkiyeparazitolderg.org/archives/archive-detail/article-preview/nvestigation-of-idientamoeba-fragilis-i-frequency-/48457 dientamoeba fragilispcrenterobius vermicularistrichrome |
| spellingShingle | İbrahim Yıldız Sema Ertuğ Evren Tileklioğlu Erdoğan Malatyalı Özgür Güçlü Hatice Ertabaklar Investigation of Dientamoeba fragilis Frequency in Faecal Samples of Patients with Enterobius vermicularis Infection by Polymerase Chain Reaction Türkiye Parazitoloji Dergisi dientamoeba fragilis pcr enterobius vermicularis trichrome |
| title | Investigation of Dientamoeba fragilis Frequency in Faecal Samples of Patients with Enterobius vermicularis Infection by Polymerase Chain Reaction |
| title_full | Investigation of Dientamoeba fragilis Frequency in Faecal Samples of Patients with Enterobius vermicularis Infection by Polymerase Chain Reaction |
| title_fullStr | Investigation of Dientamoeba fragilis Frequency in Faecal Samples of Patients with Enterobius vermicularis Infection by Polymerase Chain Reaction |
| title_full_unstemmed | Investigation of Dientamoeba fragilis Frequency in Faecal Samples of Patients with Enterobius vermicularis Infection by Polymerase Chain Reaction |
| title_short | Investigation of Dientamoeba fragilis Frequency in Faecal Samples of Patients with Enterobius vermicularis Infection by Polymerase Chain Reaction |
| title_sort | investigation of dientamoeba fragilis frequency in faecal samples of patients with enterobius vermicularis infection by polymerase chain reaction |
| topic | dientamoeba fragilis pcr enterobius vermicularis trichrome |
| url |
http://www.turkiyeparazitolderg.org/archives/archive-detail/article-preview/nvestigation-of-idientamoeba-fragilis-i-frequency-/48457
|
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