Bivalent rotavirus VP4∗ stimulates protective antibodies against common genotypes of human rotaviruses
Summary: Non-replicating rotavirus vaccines are an alternative strategy to improve the efficacy and safety of rotavirus vaccines. The spike protein VP4, which could be enzymatically cleaved into VP8∗ and VP5∗, is an ideal target for the development of recombinant rotavirus vaccine. In our previous s...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-10-01
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| Series: | iScience |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2589004222013712 |
| _version_ | 1811204381149757440 |
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| author | Guoxing Luo Yuanjun Zeng Han Yang Yijian Li Lianwei Yang Cao Li Feibo Song Shiyin Zhang Tingdong Li Shengxiang Ge Jun Zhang Ningshao Xia |
| author_facet | Guoxing Luo Yuanjun Zeng Han Yang Yijian Li Lianwei Yang Cao Li Feibo Song Shiyin Zhang Tingdong Li Shengxiang Ge Jun Zhang Ningshao Xia |
| author_sort | Guoxing Luo |
| collection | DOAJ |
| description | Summary: Non-replicating rotavirus vaccines are an alternative strategy to improve the efficacy and safety of rotavirus vaccines. The spike protein VP4, which could be enzymatically cleaved into VP8∗ and VP5∗, is an ideal target for the development of recombinant rotavirus vaccine. In our previous studies, we demonstrated that the truncated VP4 (aa26-476, VP4∗) could be a more viable vaccine candidate compared to VP8∗ and VP5∗. Here, to develop a human rotavirus vaccine, the VP4∗ proteins of P[4], P[6], and P[8] genotype rotaviruses were expressed. All VP4∗ proteins can stimulate high levels of neutralizing antibodies in both guinea pigs and rabbits when formulated in aluminum adjuvant. Furthermore, bivalent VP4∗-based vaccine (P[8] + P[6]-VP4∗) can stimulate high levels of neutralizing antibodies against various genotypes of rotavirus with no significant difference as compared to the trivalent vaccines. Therefore, bivalent VP4∗ has the potential to be a viable rotavirus vaccine candidate for further development. |
| first_indexed | 2024-04-12T03:12:21Z |
| format | Article |
| id | doaj.art-7e88edbcc6dc4ee69cd2b0485b40d163 |
| institution | Directory Open Access Journal |
| issn | 2589-0042 |
| language | English |
| last_indexed | 2024-04-12T03:12:21Z |
| publishDate | 2022-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | iScience |
| spelling | doaj.art-7e88edbcc6dc4ee69cd2b0485b40d1632022-12-22T03:50:19ZengElsevieriScience2589-00422022-10-012510105099Bivalent rotavirus VP4∗ stimulates protective antibodies against common genotypes of human rotavirusesGuoxing Luo0Yuanjun Zeng1Han Yang2Yijian Li3Lianwei Yang4Cao Li5Feibo Song6Shiyin Zhang7Tingdong Li8Shengxiang Ge9Jun Zhang10Ningshao Xia11State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Public Health, Xiamen University, Xiamen 361102, ChinaState Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Public Health, Xiamen University, Xiamen 361102, ChinaState Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Public Health, Xiamen University, Xiamen 361102, ChinaCollege of Life Sciences, University of Chinese Academy of Sciences, Beijing 100049, ChinaR&D Department of Xiamen Innodx Biotechnology Co., Ltd, Xiamen 361000, ChinaState Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Public Health, Xiamen University, Xiamen 361102, ChinaNational Institute of Diagnostics and Vaccine Development in Infectious Diseases, School of Life Sciences, Xiamen University, Xiamen 361102, ChinaState Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Public Health, Xiamen University, Xiamen 361102, ChinaState Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Public Health, Xiamen University, Xiamen 361102, China; Corresponding authorState Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Public Health, Xiamen University, Xiamen 361102, China; Corresponding authorState Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Public Health, Xiamen University, Xiamen 361102, ChinaState Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Public Health, Xiamen University, Xiamen 361102, China; National Institute of Diagnostics and Vaccine Development in Infectious Diseases, School of Life Sciences, Xiamen University, Xiamen 361102, ChinaSummary: Non-replicating rotavirus vaccines are an alternative strategy to improve the efficacy and safety of rotavirus vaccines. The spike protein VP4, which could be enzymatically cleaved into VP8∗ and VP5∗, is an ideal target for the development of recombinant rotavirus vaccine. In our previous studies, we demonstrated that the truncated VP4 (aa26-476, VP4∗) could be a more viable vaccine candidate compared to VP8∗ and VP5∗. Here, to develop a human rotavirus vaccine, the VP4∗ proteins of P[4], P[6], and P[8] genotype rotaviruses were expressed. All VP4∗ proteins can stimulate high levels of neutralizing antibodies in both guinea pigs and rabbits when formulated in aluminum adjuvant. Furthermore, bivalent VP4∗-based vaccine (P[8] + P[6]-VP4∗) can stimulate high levels of neutralizing antibodies against various genotypes of rotavirus with no significant difference as compared to the trivalent vaccines. Therefore, bivalent VP4∗ has the potential to be a viable rotavirus vaccine candidate for further development.http://www.sciencedirect.com/science/article/pii/S2589004222013712Biological sciencesMicrobiologyVirologyViral microbiology |
| spellingShingle | Guoxing Luo Yuanjun Zeng Han Yang Yijian Li Lianwei Yang Cao Li Feibo Song Shiyin Zhang Tingdong Li Shengxiang Ge Jun Zhang Ningshao Xia Bivalent rotavirus VP4∗ stimulates protective antibodies against common genotypes of human rotaviruses iScience Biological sciences Microbiology Virology Viral microbiology |
| title | Bivalent rotavirus VP4∗ stimulates protective antibodies against common genotypes of human rotaviruses |
| title_full | Bivalent rotavirus VP4∗ stimulates protective antibodies against common genotypes of human rotaviruses |
| title_fullStr | Bivalent rotavirus VP4∗ stimulates protective antibodies against common genotypes of human rotaviruses |
| title_full_unstemmed | Bivalent rotavirus VP4∗ stimulates protective antibodies against common genotypes of human rotaviruses |
| title_short | Bivalent rotavirus VP4∗ stimulates protective antibodies against common genotypes of human rotaviruses |
| title_sort | bivalent rotavirus vp4∗ stimulates protective antibodies against common genotypes of human rotaviruses |
| topic | Biological sciences Microbiology Virology Viral microbiology |
| url | http://www.sciencedirect.com/science/article/pii/S2589004222013712 |
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