Molecular Mapping of Urinary Complement Peptides in Kidney Diseases
Defective complement activation has been associated with various types of kidney disease. This led to the hypothesis that specific urine complement fragments may be associated with kidney disease etiologies, and disease progression may be reflected by changes in these complement fragments. We invest...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-12-01
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| Series: | Proteomes |
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| Online Access: | https://www.mdpi.com/2227-7382/9/4/49 |
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| author | Ralph Wendt Justyna Siwy Tianlin He Agnieszka Latosinska Thorsten Wiech Peter F. Zipfel Aggeliki Tserga Antonia Vlahou Harald Rupprecht Lorenzo Catanese Harald Mischak Joachim Beige |
| author_facet | Ralph Wendt Justyna Siwy Tianlin He Agnieszka Latosinska Thorsten Wiech Peter F. Zipfel Aggeliki Tserga Antonia Vlahou Harald Rupprecht Lorenzo Catanese Harald Mischak Joachim Beige |
| author_sort | Ralph Wendt |
| collection | DOAJ |
| description | Defective complement activation has been associated with various types of kidney disease. This led to the hypothesis that specific urine complement fragments may be associated with kidney disease etiologies, and disease progression may be reflected by changes in these complement fragments. We investigated the occurrence of complement fragments in urine, their association with kidney function and disease etiology in 16,027 subjects, using mass spectrometry based peptidomics data from the Human Urinary Proteome/Peptidome Database. Twenty-three different urinary peptides originating from complement proteins C3, C4 and factor B (CFB) could be identified. Most C3-derived peptides showed inverse association with estimated glomerular filtration rate (eGFR), while the majority of peptides derived from CFB demonstrated positive association with eGFR. Several peptides derived from the complement proteins C3, C4 and CFB were found significantly associated with specific kidney disease etiologies. These peptides may depict disease-specific complement activation and could serve as non-invasive biomarkers to support development of complement interventions through assessing complement activity for patients’ stratification and monitoring of drug impact. Further investigation of these complement peptides may provide additional insight into disease pathophysiology and could possibly guide therapeutic decisions, especially when targeting complement factors. |
| first_indexed | 2024-03-10T03:13:41Z |
| format | Article |
| id | doaj.art-7e8ebd244afd4f9091edf50c0554b3e4 |
| institution | Directory Open Access Journal |
| issn | 2227-7382 |
| language | English |
| last_indexed | 2024-03-10T03:13:41Z |
| publishDate | 2021-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Proteomes |
| spelling | doaj.art-7e8ebd244afd4f9091edf50c0554b3e42023-11-23T10:20:07ZengMDPI AGProteomes2227-73822021-12-01944910.3390/proteomes9040049Molecular Mapping of Urinary Complement Peptides in Kidney DiseasesRalph Wendt0Justyna Siwy1Tianlin He2Agnieszka Latosinska3Thorsten Wiech4Peter F. Zipfel5Aggeliki Tserga6Antonia Vlahou7Harald Rupprecht8Lorenzo Catanese9Harald Mischak10Joachim Beige11Department of Nephrology and Kuratorium for Dialysis and Transplantation (KfH) Renal Unit, Hospital St. Georg, 04129 Leipzig, GermanyMosaiques Diagnostics GmbH, 30659 Hannover, GermanyMosaiques Diagnostics GmbH, 30659 Hannover, GermanyMosaiques Diagnostics GmbH, 30659 Hannover, GermanyNephropathology Section, Institute of Pathology, University Medical Center, 20246 Hamburg, GermanyInstitute of Microbiology, Friedrich-Schiller-University, 07743 Jena, GermanyBiomedical Research Foundation, Academy of Athens, Department of Biotechnology, 11527 Athens, GreeceBiomedical Research Foundation, Academy of Athens, Department of Biotechnology, 11527 Athens, GreeceDepartment of Nephrology, Klinikum Bayreuth GmbH, 95447 Bayreuth, GermanyDepartment of Nephrology, Klinikum Bayreuth GmbH, 95447 Bayreuth, GermanyMosaiques Diagnostics GmbH, 30659 Hannover, GermanyDepartment of Nephrology and Kuratorium for Dialysis and Transplantation (KfH) Renal Unit, Hospital St. Georg, 04129 Leipzig, GermanyDefective complement activation has been associated with various types of kidney disease. This led to the hypothesis that specific urine complement fragments may be associated with kidney disease etiologies, and disease progression may be reflected by changes in these complement fragments. We investigated the occurrence of complement fragments in urine, their association with kidney function and disease etiology in 16,027 subjects, using mass spectrometry based peptidomics data from the Human Urinary Proteome/Peptidome Database. Twenty-three different urinary peptides originating from complement proteins C3, C4 and factor B (CFB) could be identified. Most C3-derived peptides showed inverse association with estimated glomerular filtration rate (eGFR), while the majority of peptides derived from CFB demonstrated positive association with eGFR. Several peptides derived from the complement proteins C3, C4 and CFB were found significantly associated with specific kidney disease etiologies. These peptides may depict disease-specific complement activation and could serve as non-invasive biomarkers to support development of complement interventions through assessing complement activity for patients’ stratification and monitoring of drug impact. Further investigation of these complement peptides may provide additional insight into disease pathophysiology and could possibly guide therapeutic decisions, especially when targeting complement factors.https://www.mdpi.com/2227-7382/9/4/49complementpeptideurinebiomarkerkidney diseaseproteomics |
| spellingShingle | Ralph Wendt Justyna Siwy Tianlin He Agnieszka Latosinska Thorsten Wiech Peter F. Zipfel Aggeliki Tserga Antonia Vlahou Harald Rupprecht Lorenzo Catanese Harald Mischak Joachim Beige Molecular Mapping of Urinary Complement Peptides in Kidney Diseases Proteomes complement peptide urine biomarker kidney disease proteomics |
| title | Molecular Mapping of Urinary Complement Peptides in Kidney Diseases |
| title_full | Molecular Mapping of Urinary Complement Peptides in Kidney Diseases |
| title_fullStr | Molecular Mapping of Urinary Complement Peptides in Kidney Diseases |
| title_full_unstemmed | Molecular Mapping of Urinary Complement Peptides in Kidney Diseases |
| title_short | Molecular Mapping of Urinary Complement Peptides in Kidney Diseases |
| title_sort | molecular mapping of urinary complement peptides in kidney diseases |
| topic | complement peptide urine biomarker kidney disease proteomics |
| url | https://www.mdpi.com/2227-7382/9/4/49 |
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