Evaluating Relative Retention of Polar Stationary Phases in Hydrophilic Interaction Chromatography
A large number of polar stationary phases with diverse chemistry have been developed for various applications in hydrophilic interaction chromatography (HILIC). However, column manufacturers employ different testing procedures to evaluate retention of the polar stationary phases. This renders the re...
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| Format: | Article |
| Language: | English |
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MDPI AG
2019-08-01
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| Series: | Separations |
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| Online Access: | https://www.mdpi.com/2297-8739/6/3/42 |
| _version_ | 1811303264481705984 |
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| author | Yong Guo Nidhi Bhalodia Bassel Fattal |
| author_facet | Yong Guo Nidhi Bhalodia Bassel Fattal |
| author_sort | Yong Guo |
| collection | DOAJ |
| description | A large number of polar stationary phases with diverse chemistry have been developed for various applications in hydrophilic interaction chromatography (HILIC). However, column manufacturers employ different testing procedures to evaluate retention of the polar stationary phases. This renders the retention data impossible for comparison and makes it difficult for the users to select the right stationary phase based on retention. We have evaluated 25 polar stationary phases using cytosine and uracil as the model compounds in various mobile phase conditions. These stationary phases show a wide range of retention characteristics for the model compounds. The ranking of the stationary phases does not change drastically with the acetonitrile level in the mobile phase. |
| first_indexed | 2024-04-13T07:43:47Z |
| format | Article |
| id | doaj.art-7e9340e9f7c64c7cb00183aa1c41e599 |
| institution | Directory Open Access Journal |
| issn | 2297-8739 |
| language | English |
| last_indexed | 2024-04-13T07:43:47Z |
| publishDate | 2019-08-01 |
| publisher | MDPI AG |
| record_format | Article |
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| spelling | doaj.art-7e9340e9f7c64c7cb00183aa1c41e5992022-12-22T02:55:47ZengMDPI AGSeparations2297-87392019-08-01634210.3390/separations6030042separations6030042Evaluating Relative Retention of Polar Stationary Phases in Hydrophilic Interaction ChromatographyYong Guo0Nidhi Bhalodia1Bassel Fattal2School of Pharmacy and Health Sciences, Fairleigh Dickinson University, Florham Park, NJ 07932, USASchool of Pharmacy and Health Sciences, Fairleigh Dickinson University, Florham Park, NJ 07932, USASchool of Pharmacy and Health Sciences, Fairleigh Dickinson University, Florham Park, NJ 07932, USAA large number of polar stationary phases with diverse chemistry have been developed for various applications in hydrophilic interaction chromatography (HILIC). However, column manufacturers employ different testing procedures to evaluate retention of the polar stationary phases. This renders the retention data impossible for comparison and makes it difficult for the users to select the right stationary phase based on retention. We have evaluated 25 polar stationary phases using cytosine and uracil as the model compounds in various mobile phase conditions. These stationary phases show a wide range of retention characteristics for the model compounds. The ranking of the stationary phases does not change drastically with the acetonitrile level in the mobile phase.https://www.mdpi.com/2297-8739/6/3/42stationary phaseretentioncytosine |
| spellingShingle | Yong Guo Nidhi Bhalodia Bassel Fattal Evaluating Relative Retention of Polar Stationary Phases in Hydrophilic Interaction Chromatography Separations stationary phase retention cytosine |
| title | Evaluating Relative Retention of Polar Stationary Phases in Hydrophilic Interaction Chromatography |
| title_full | Evaluating Relative Retention of Polar Stationary Phases in Hydrophilic Interaction Chromatography |
| title_fullStr | Evaluating Relative Retention of Polar Stationary Phases in Hydrophilic Interaction Chromatography |
| title_full_unstemmed | Evaluating Relative Retention of Polar Stationary Phases in Hydrophilic Interaction Chromatography |
| title_short | Evaluating Relative Retention of Polar Stationary Phases in Hydrophilic Interaction Chromatography |
| title_sort | evaluating relative retention of polar stationary phases in hydrophilic interaction chromatography |
| topic | stationary phase retention cytosine |
| url | https://www.mdpi.com/2297-8739/6/3/42 |
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