Mechano-Transduction Boosts the Aging Effects in Human Erythrocytes Submitted to Mechanical Stimulation
Erythrocytes’ aging and mechano-transduction are fundamental cellular pathways that determine the red blood cells’ (RBCs) behavior and function. The aging pattern can be influenced, in morphological, biochemical, and metabolic terms by the environmental conditions. In this paper, we studied the effe...
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MDPI AG
2022-09-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/17/10180 |
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| author | Simone Dinarelli Giovanni Longo Antonio Francioso Luciana Mosca Marco Girasole |
| author_facet | Simone Dinarelli Giovanni Longo Antonio Francioso Luciana Mosca Marco Girasole |
| author_sort | Simone Dinarelli |
| collection | DOAJ |
| description | Erythrocytes’ aging and mechano-transduction are fundamental cellular pathways that determine the red blood cells’ (RBCs) behavior and function. The aging pattern can be influenced, in morphological, biochemical, and metabolic terms by the environmental conditions. In this paper, we studied the effect of a moderate mechanical stimulation applied through external shaking during the RBCs aging and revealed a strong acceleration of the aging pattern induced by such stimulation. Moreover, we evaluated the behavior of the main cellular effectors and resources in the presence of drugs (diamide) or of specific inhibitors of the mechano-transduction (probenecid, carbenoxolone, and glibenclamide). This approach provided the first evidence of a direct cross-correlation between aging and mechano-transduction and permitted an evaluation of the overall metabolic regulation and of the insurgence of specific morphological features, such as micro-vesicles and roughness alterations. Overall, for the first time the present data provided a schematic to understand the integration of distinct complex patterns in a comprehensive view of the cell and of its interactions with the environment. Mechano-transduction produces structural effects that are correlated with the stimulation and the strength of the environmental stimulation is paramount to effectively activate and trigger the biological cascades initiated by the mechano-sensing. |
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| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T01:41:26Z |
| publishDate | 2022-09-01 |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-7e9733a117e841958a72dc850adbd2922023-11-23T13:24:16ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-09-0123171018010.3390/ijms231710180Mechano-Transduction Boosts the Aging Effects in Human Erythrocytes Submitted to Mechanical StimulationSimone Dinarelli0Giovanni Longo1Antonio Francioso2Luciana Mosca3Marco Girasole4Institute for the Structure of the Matter (ISM), Italian National Research Council (CNR), Via Fosso del Cavaliere 100, 00133 Roma, ItalyInstitute for the Structure of the Matter (ISM), Italian National Research Council (CNR), Via Fosso del Cavaliere 100, 00133 Roma, ItalyDepartment Biochemical Sciences “A. Rossi-Fanelli”, Sapienza University of Rome, Piazzale A. Moro 5, 00185 Roma, ItalyDepartment Biochemical Sciences “A. Rossi-Fanelli”, Sapienza University of Rome, Piazzale A. Moro 5, 00185 Roma, ItalyInstitute for the Structure of the Matter (ISM), Italian National Research Council (CNR), Via Fosso del Cavaliere 100, 00133 Roma, ItalyErythrocytes’ aging and mechano-transduction are fundamental cellular pathways that determine the red blood cells’ (RBCs) behavior and function. The aging pattern can be influenced, in morphological, biochemical, and metabolic terms by the environmental conditions. In this paper, we studied the effect of a moderate mechanical stimulation applied through external shaking during the RBCs aging and revealed a strong acceleration of the aging pattern induced by such stimulation. Moreover, we evaluated the behavior of the main cellular effectors and resources in the presence of drugs (diamide) or of specific inhibitors of the mechano-transduction (probenecid, carbenoxolone, and glibenclamide). This approach provided the first evidence of a direct cross-correlation between aging and mechano-transduction and permitted an evaluation of the overall metabolic regulation and of the insurgence of specific morphological features, such as micro-vesicles and roughness alterations. Overall, for the first time the present data provided a schematic to understand the integration of distinct complex patterns in a comprehensive view of the cell and of its interactions with the environment. Mechano-transduction produces structural effects that are correlated with the stimulation and the strength of the environmental stimulation is paramount to effectively activate and trigger the biological cascades initiated by the mechano-sensing.https://www.mdpi.com/1422-0067/23/17/10180erythrocytescell agingmechanical sensingmechano-transductionAFMmembrane roughness |
| spellingShingle | Simone Dinarelli Giovanni Longo Antonio Francioso Luciana Mosca Marco Girasole Mechano-Transduction Boosts the Aging Effects in Human Erythrocytes Submitted to Mechanical Stimulation International Journal of Molecular Sciences erythrocytes cell aging mechanical sensing mechano-transduction AFM membrane roughness |
| title | Mechano-Transduction Boosts the Aging Effects in Human Erythrocytes Submitted to Mechanical Stimulation |
| title_full | Mechano-Transduction Boosts the Aging Effects in Human Erythrocytes Submitted to Mechanical Stimulation |
| title_fullStr | Mechano-Transduction Boosts the Aging Effects in Human Erythrocytes Submitted to Mechanical Stimulation |
| title_full_unstemmed | Mechano-Transduction Boosts the Aging Effects in Human Erythrocytes Submitted to Mechanical Stimulation |
| title_short | Mechano-Transduction Boosts the Aging Effects in Human Erythrocytes Submitted to Mechanical Stimulation |
| title_sort | mechano transduction boosts the aging effects in human erythrocytes submitted to mechanical stimulation |
| topic | erythrocytes cell aging mechanical sensing mechano-transduction AFM membrane roughness |
| url | https://www.mdpi.com/1422-0067/23/17/10180 |
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