Gene expression profiling of circulating CD133+ cells of hepatocellular carcinoma patients associated with HCV infection
Aim: Identifying the genetic expression profile of CD133+ cells from HCC patients compared to CD133+ cells from healthy volunteers that may contribute in hepatocarcinogenesis process. Method: Circulating CD133+ cells were sorted from the peripheral blood of HCC patients as well as from healthy volun...
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| Format: | Article |
| Language: | English |
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SpringerOpen
2017-03-01
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| Series: | Journal of the Egyptian National Cancer Institute |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1110036216300772 |
| _version_ | 1819042884833247232 |
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| author | Abdel-Rahman N. Zekri Enas R. El-Sisi Zeinab F. Abdallah Alaa Ismail Ahmed Barakat Barakat |
| author_facet | Abdel-Rahman N. Zekri Enas R. El-Sisi Zeinab F. Abdallah Alaa Ismail Ahmed Barakat Barakat |
| author_sort | Abdel-Rahman N. Zekri |
| collection | DOAJ |
| description | Aim: Identifying the genetic expression profile of CD133+ cells from HCC patients compared to CD133+ cells from healthy volunteers that may contribute in hepatocarcinogenesis process.
Method: Circulating CD133+ cells were sorted from the peripheral blood of HCC patients as well as from healthy volunteers using magnetic activated cell sorting. The differential expression profile of stem cell related genes was performed using the Stem Cell PCR profiling assay.
Results: Data analysis of stem cells related genes in CD133+ cells of the HCC group compared to the control group showed that; CCND2, COL1A1, CTNNA1, DLL3, JAG1, KRT15, MYC, NOTCH2, T and TERT were up-regulated (fold change = 80, 68.6, 6.67, 7.22, 3.8, 15.2, 14.5, 105.6, 26.6 and 99 respectively while only CD3D was down-regulated (fold change = 0.055) in HCC patients. However, after application of Beferroni correction to adjust P-value; KRT15 was the only gene that was significantly over expressed in CD133+ cells of HCC compared to control group (P-value = 0.012).
Conclusion: KRT15 can be used to differentiate between circulating CD133+ cells from HCC group and control group. However, further study may be needed to confirm on the protein level. |
| first_indexed | 2024-12-21T09:47:59Z |
| format | Article |
| id | doaj.art-7e980f3ef0184e40920a9181f95fca1a |
| institution | Directory Open Access Journal |
| issn | 1110-0362 |
| language | English |
| last_indexed | 2024-12-21T09:47:59Z |
| publishDate | 2017-03-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Journal of the Egyptian National Cancer Institute |
| spelling | doaj.art-7e980f3ef0184e40920a9181f95fca1a2022-12-21T19:08:16ZengSpringerOpenJournal of the Egyptian National Cancer Institute1110-03622017-03-01291192410.1016/j.jnci.2016.12.002Gene expression profiling of circulating CD133+ cells of hepatocellular carcinoma patients associated with HCV infectionAbdel-Rahman N. Zekri0Enas R. El-Sisi1Zeinab F. Abdallah2Alaa Ismail3Ahmed Barakat Barakat4Molecular Virology and Immunology Unit, Cancer Biology Department, National Cancer Institute, Cairo University, Cairo, EgyptMolecular Virology and Immunology Unit, Cancer Biology Department, National Cancer Institute, Cairo University, Cairo, EgyptMolecular Virology and Immunology Unit, Cancer Biology Department, National Cancer Institute, Cairo University, Cairo, EgyptSurgery Department, Faculty of Medicine, Ain Shams University, Cairo, EgyptMicrobiology Department, Faculty of Science, Ain Shams University, Cairo, EgyptAim: Identifying the genetic expression profile of CD133+ cells from HCC patients compared to CD133+ cells from healthy volunteers that may contribute in hepatocarcinogenesis process. Method: Circulating CD133+ cells were sorted from the peripheral blood of HCC patients as well as from healthy volunteers using magnetic activated cell sorting. The differential expression profile of stem cell related genes was performed using the Stem Cell PCR profiling assay. Results: Data analysis of stem cells related genes in CD133+ cells of the HCC group compared to the control group showed that; CCND2, COL1A1, CTNNA1, DLL3, JAG1, KRT15, MYC, NOTCH2, T and TERT were up-regulated (fold change = 80, 68.6, 6.67, 7.22, 3.8, 15.2, 14.5, 105.6, 26.6 and 99 respectively while only CD3D was down-regulated (fold change = 0.055) in HCC patients. However, after application of Beferroni correction to adjust P-value; KRT15 was the only gene that was significantly over expressed in CD133+ cells of HCC compared to control group (P-value = 0.012). Conclusion: KRT15 can be used to differentiate between circulating CD133+ cells from HCC group and control group. However, further study may be needed to confirm on the protein level.http://www.sciencedirect.com/science/article/pii/S1110036216300772Hepatocellular carcinomaCD133+ cellsHCV |
| spellingShingle | Abdel-Rahman N. Zekri Enas R. El-Sisi Zeinab F. Abdallah Alaa Ismail Ahmed Barakat Barakat Gene expression profiling of circulating CD133+ cells of hepatocellular carcinoma patients associated with HCV infection Journal of the Egyptian National Cancer Institute Hepatocellular carcinoma CD133+ cells HCV |
| title | Gene expression profiling of circulating CD133+ cells of hepatocellular carcinoma patients associated with HCV infection |
| title_full | Gene expression profiling of circulating CD133+ cells of hepatocellular carcinoma patients associated with HCV infection |
| title_fullStr | Gene expression profiling of circulating CD133+ cells of hepatocellular carcinoma patients associated with HCV infection |
| title_full_unstemmed | Gene expression profiling of circulating CD133+ cells of hepatocellular carcinoma patients associated with HCV infection |
| title_short | Gene expression profiling of circulating CD133+ cells of hepatocellular carcinoma patients associated with HCV infection |
| title_sort | gene expression profiling of circulating cd133 cells of hepatocellular carcinoma patients associated with hcv infection |
| topic | Hepatocellular carcinoma CD133+ cells HCV |
| url | http://www.sciencedirect.com/science/article/pii/S1110036216300772 |
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