Development of Optical Biosensor Using Protein A-Conjugated Chitosan–Gold Nanoparticles for Diagnosis of Cystic Echinococcosis
Human echinococcosis is a serious parasitic diseasethat still affects millions of people in many parts of the world. Since it can offer a critical threat to people’s health, it is important to discover a rapid, convenient, and economical method for detection. Herein, we propose a novel point of care...
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MDPI AG
2021-04-01
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| Series: | Biosensors |
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| Online Access: | https://www.mdpi.com/2079-6374/11/5/134 |
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| author | Hanie Safarpour Hasan Majdi Ali Masjedi Abdol Sattar Pagheh Maria de Lourdes Pereira Sonia M. Rodrigues Oliveira Ehsan Ahmadpour |
| author_facet | Hanie Safarpour Hasan Majdi Ali Masjedi Abdol Sattar Pagheh Maria de Lourdes Pereira Sonia M. Rodrigues Oliveira Ehsan Ahmadpour |
| author_sort | Hanie Safarpour |
| collection | DOAJ |
| description | Human echinococcosis is a serious parasitic diseasethat still affects millions of people in many parts of the world. Since it can offer a critical threat to people’s health, it is important to discover a rapid, convenient, and economical method for detection. Herein, we propose a novel point of care assay, namely, an enhanced immuno-dot-blot assay for diagnosis of cystic echinococcosis (hydatidosis). This method is based on the formation of a sandwich complex between a goldnanoprobe (chitosan–gold nanoparticleprotein A) and hydatid cyst antigen (Ag B), which holds anti-Ag B antibodies. Briefly, protein A was conjugated to chitosan–gold nanoparticles via glutaraldehyde chemistry. Then, Ag B was immobilized on the surface of a nitrocellulose membrane, which was followed by the addition of the sera sample and gold nanoprobes. The positive signals were easily detectable by naked eye. The signal intensity of this biosensor was proportional to the concentration of active anti-<i>Echinococcus granulosus</i> antibodies on the surface of the nanoparticles, titer of antibodies in the sera samples, and concentration of Ag B coated on the nitrocellulose membrane. The minimum concentration to use the protein A for conjugation to detect titer of anti-<i>Echinococcus</i> IgGand the concentration of Ag B coated in nitrocellulose membrane were 0.5 and 0.3 mg/mL, respectively. This enhanced immuno-dot-blot assay offers a simple diagnostic technique withoutthe need for expensive equipment for diagnosis of echinococcosis. |
| first_indexed | 2024-03-10T11:59:01Z |
| format | Article |
| id | doaj.art-7e99b074c5bb4e32b93ce0e579a009e6 |
| institution | Directory Open Access Journal |
| issn | 2079-6374 |
| language | English |
| last_indexed | 2024-03-10T11:59:01Z |
| publishDate | 2021-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biosensors |
| spelling | doaj.art-7e99b074c5bb4e32b93ce0e579a009e62023-11-21T17:04:53ZengMDPI AGBiosensors2079-63742021-04-0111513410.3390/bios11050134Development of Optical Biosensor Using Protein A-Conjugated Chitosan–Gold Nanoparticles for Diagnosis of Cystic EchinococcosisHanie Safarpour0Hasan Majdi1Ali Masjedi2Abdol Sattar Pagheh3Maria de Lourdes Pereira4Sonia M. Rodrigues Oliveira5Ehsan Ahmadpour6Infectious and Tropical Disease Research Center, Tabriz University of Medical Sciences, Tabriz 51666-14766, IranDrug Applied Research Center, Tabriz University of Medical Sciences, Tabriz 51666-14766, IranImmunology Research Center, Tabriz University of Medical Sciences, Tabriz 51666-14766, IranInfectious Diseases Research Center, Birjand University of Medical Sciences, Birjand 97178-53577, IranCICECO-Aveiro Institute of Materials, University of Aveiro, 3810-193 Aveiro, PortugalCICECO-Aveiro Institute of Materials, University of Aveiro, 3810-193 Aveiro, PortugalInfectious and Tropical Disease Research Center, Tabriz University of Medical Sciences, Tabriz 51666-14766, IranHuman echinococcosis is a serious parasitic diseasethat still affects millions of people in many parts of the world. Since it can offer a critical threat to people’s health, it is important to discover a rapid, convenient, and economical method for detection. Herein, we propose a novel point of care assay, namely, an enhanced immuno-dot-blot assay for diagnosis of cystic echinococcosis (hydatidosis). This method is based on the formation of a sandwich complex between a goldnanoprobe (chitosan–gold nanoparticleprotein A) and hydatid cyst antigen (Ag B), which holds anti-Ag B antibodies. Briefly, protein A was conjugated to chitosan–gold nanoparticles via glutaraldehyde chemistry. Then, Ag B was immobilized on the surface of a nitrocellulose membrane, which was followed by the addition of the sera sample and gold nanoprobes. The positive signals were easily detectable by naked eye. The signal intensity of this biosensor was proportional to the concentration of active anti-<i>Echinococcus granulosus</i> antibodies on the surface of the nanoparticles, titer of antibodies in the sera samples, and concentration of Ag B coated on the nitrocellulose membrane. The minimum concentration to use the protein A for conjugation to detect titer of anti-<i>Echinococcus</i> IgGand the concentration of Ag B coated in nitrocellulose membrane were 0.5 and 0.3 mg/mL, respectively. This enhanced immuno-dot-blot assay offers a simple diagnostic technique withoutthe need for expensive equipment for diagnosis of echinococcosis.https://www.mdpi.com/2079-6374/11/5/134<i>Echinococcus granulosus</i>hydatidosisdiagnosisdot-blotchitosan–gold nanoparticleprotein A |
| spellingShingle | Hanie Safarpour Hasan Majdi Ali Masjedi Abdol Sattar Pagheh Maria de Lourdes Pereira Sonia M. Rodrigues Oliveira Ehsan Ahmadpour Development of Optical Biosensor Using Protein A-Conjugated Chitosan–Gold Nanoparticles for Diagnosis of Cystic Echinococcosis Biosensors <i>Echinococcus granulosus</i> hydatidosis diagnosis dot-blot chitosan–gold nanoparticle protein A |
| title | Development of Optical Biosensor Using Protein A-Conjugated Chitosan–Gold Nanoparticles for Diagnosis of Cystic Echinococcosis |
| title_full | Development of Optical Biosensor Using Protein A-Conjugated Chitosan–Gold Nanoparticles for Diagnosis of Cystic Echinococcosis |
| title_fullStr | Development of Optical Biosensor Using Protein A-Conjugated Chitosan–Gold Nanoparticles for Diagnosis of Cystic Echinococcosis |
| title_full_unstemmed | Development of Optical Biosensor Using Protein A-Conjugated Chitosan–Gold Nanoparticles for Diagnosis of Cystic Echinococcosis |
| title_short | Development of Optical Biosensor Using Protein A-Conjugated Chitosan–Gold Nanoparticles for Diagnosis of Cystic Echinococcosis |
| title_sort | development of optical biosensor using protein a conjugated chitosan gold nanoparticles for diagnosis of cystic echinococcosis |
| topic | <i>Echinococcus granulosus</i> hydatidosis diagnosis dot-blot chitosan–gold nanoparticle protein A |
| url | https://www.mdpi.com/2079-6374/11/5/134 |
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