Uncovering Differences in Virulence Markers Associated with Achromobacter Species of CF and Non-CF Origin
Achromobacter spp. are recognized as emerging pathogens in hospitalized as well as in cystic fibrosis (CF) patients. From 2012 to 2015, we collected 69 clinical isolates (41 patient) of Achromobacter spp. from 13 patients with CF (CF isolates, n = 32) and 28 patients receiving care for other health...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2017-05-01
|
| Series: | Frontiers in Cellular and Infection Microbiology |
| Subjects: | |
| Online Access: | http://journal.frontiersin.org/article/10.3389/fcimb.2017.00224/full |
| _version_ | 1818602483422855168 |
|---|---|
| author | Brankica Filipic Brankica Filipic Milka Malesevic Zorica Vasiljevic Jovanka Lukic Katarina Novovic Milan Kojic Branko Jovcic Branko Jovcic |
| author_facet | Brankica Filipic Brankica Filipic Milka Malesevic Zorica Vasiljevic Jovanka Lukic Katarina Novovic Milan Kojic Branko Jovcic Branko Jovcic |
| author_sort | Brankica Filipic |
| collection | DOAJ |
| description | Achromobacter spp. are recognized as emerging pathogens in hospitalized as well as in cystic fibrosis (CF) patients. From 2012 to 2015, we collected 69 clinical isolates (41 patient) of Achromobacter spp. from 13 patients with CF (CF isolates, n = 32) and 28 patients receiving care for other health conditions (non-CF isolates, n = 37). Molecular epidemiology and virulence potential of isolates were examined. Antimicrobial susceptibility, motility, ability to form biofilms and binding affinity to mucin, collagen, and fibronectin were tested to assess their virulence traits. The nrdA gene sequencing showed that A. xylosoxidans was the most prevalent species in both CF and non-CF patients. CF patients were also colonized with A. dolens/A. ruhlandii, A. insuavis, and A. spiritinus strains while non-CF group was somewhat less heterogenous, although A. insuavis, A. insolitus, and A. piechaudii strains were detected beside A. xylosoxidans. Three strains displayed clonal distribution, one among patients from the CF group and two among non-CF patients. No significant differences in susceptibility to antimicrobials were observed between CF and non-CF patients. About one third of the isolates were classified as strong biofilm producers, and the proportion of CF and non-CF isolates with the ability to form biofilm was almost identical. CF isolates were less motile compared to the non-CF group and no correlation was found between swimming phenotype and biofilm formation. On the other hand, CF isolates exhibited higher affinity to bind mucin, collagen, and fibronectin. In generall, CF isolates from our study exhibited in vitro properties that could be of importance for the colonization of CF patients. |
| first_indexed | 2024-12-16T13:08:00Z |
| format | Article |
| id | doaj.art-7e9b0378c7ad4edb9d149f4a578cfe39 |
| institution | Directory Open Access Journal |
| issn | 2235-2988 |
| language | English |
| last_indexed | 2024-12-16T13:08:00Z |
| publishDate | 2017-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cellular and Infection Microbiology |
| spelling | doaj.art-7e9b0378c7ad4edb9d149f4a578cfe392022-12-21T22:30:41ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882017-05-01710.3389/fcimb.2017.00224251454Uncovering Differences in Virulence Markers Associated with Achromobacter Species of CF and Non-CF OriginBrankica Filipic0Brankica Filipic1Milka Malesevic2Zorica Vasiljevic3Jovanka Lukic4Katarina Novovic5Milan Kojic6Branko Jovcic7Branko Jovcic8Faculty of Pharmacy, University of BelgradeBelgrade, SerbiaInstitute of Molecular Genetics and Genetic Engineering, University of BelgradeBelgrade, SerbiaInstitute of Molecular Genetics and Genetic Engineering, University of BelgradeBelgrade, SerbiaThe Institute for Health Protection of Mother and Child SerbiaBelgrade, SerbiaInstitute of Molecular Genetics and Genetic Engineering, University of BelgradeBelgrade, SerbiaInstitute of Molecular Genetics and Genetic Engineering, University of BelgradeBelgrade, SerbiaInstitute of Molecular Genetics and Genetic Engineering, University of BelgradeBelgrade, SerbiaInstitute of Molecular Genetics and Genetic Engineering, University of BelgradeBelgrade, SerbiaFaculty of Biology, University of BelgradeBelgrade, SerbiaAchromobacter spp. are recognized as emerging pathogens in hospitalized as well as in cystic fibrosis (CF) patients. From 2012 to 2015, we collected 69 clinical isolates (41 patient) of Achromobacter spp. from 13 patients with CF (CF isolates, n = 32) and 28 patients receiving care for other health conditions (non-CF isolates, n = 37). Molecular epidemiology and virulence potential of isolates were examined. Antimicrobial susceptibility, motility, ability to form biofilms and binding affinity to mucin, collagen, and fibronectin were tested to assess their virulence traits. The nrdA gene sequencing showed that A. xylosoxidans was the most prevalent species in both CF and non-CF patients. CF patients were also colonized with A. dolens/A. ruhlandii, A. insuavis, and A. spiritinus strains while non-CF group was somewhat less heterogenous, although A. insuavis, A. insolitus, and A. piechaudii strains were detected beside A. xylosoxidans. Three strains displayed clonal distribution, one among patients from the CF group and two among non-CF patients. No significant differences in susceptibility to antimicrobials were observed between CF and non-CF patients. About one third of the isolates were classified as strong biofilm producers, and the proportion of CF and non-CF isolates with the ability to form biofilm was almost identical. CF isolates were less motile compared to the non-CF group and no correlation was found between swimming phenotype and biofilm formation. On the other hand, CF isolates exhibited higher affinity to bind mucin, collagen, and fibronectin. In generall, CF isolates from our study exhibited in vitro properties that could be of importance for the colonization of CF patients.http://journal.frontiersin.org/article/10.3389/fcimb.2017.00224/fullcystic fibrosisAchromobacter spp.nrdAantimicrobial resistancevirulence traits |
| spellingShingle | Brankica Filipic Brankica Filipic Milka Malesevic Zorica Vasiljevic Jovanka Lukic Katarina Novovic Milan Kojic Branko Jovcic Branko Jovcic Uncovering Differences in Virulence Markers Associated with Achromobacter Species of CF and Non-CF Origin Frontiers in Cellular and Infection Microbiology cystic fibrosis Achromobacter spp. nrdA antimicrobial resistance virulence traits |
| title | Uncovering Differences in Virulence Markers Associated with Achromobacter Species of CF and Non-CF Origin |
| title_full | Uncovering Differences in Virulence Markers Associated with Achromobacter Species of CF and Non-CF Origin |
| title_fullStr | Uncovering Differences in Virulence Markers Associated with Achromobacter Species of CF and Non-CF Origin |
| title_full_unstemmed | Uncovering Differences in Virulence Markers Associated with Achromobacter Species of CF and Non-CF Origin |
| title_short | Uncovering Differences in Virulence Markers Associated with Achromobacter Species of CF and Non-CF Origin |
| title_sort | uncovering differences in virulence markers associated with achromobacter species of cf and non cf origin |
| topic | cystic fibrosis Achromobacter spp. nrdA antimicrobial resistance virulence traits |
| url | http://journal.frontiersin.org/article/10.3389/fcimb.2017.00224/full |
| work_keys_str_mv | AT brankicafilipic uncoveringdifferencesinvirulencemarkersassociatedwithachromobacterspeciesofcfandnoncforigin AT brankicafilipic uncoveringdifferencesinvirulencemarkersassociatedwithachromobacterspeciesofcfandnoncforigin AT milkamalesevic uncoveringdifferencesinvirulencemarkersassociatedwithachromobacterspeciesofcfandnoncforigin AT zoricavasiljevic uncoveringdifferencesinvirulencemarkersassociatedwithachromobacterspeciesofcfandnoncforigin AT jovankalukic uncoveringdifferencesinvirulencemarkersassociatedwithachromobacterspeciesofcfandnoncforigin AT katarinanovovic uncoveringdifferencesinvirulencemarkersassociatedwithachromobacterspeciesofcfandnoncforigin AT milankojic uncoveringdifferencesinvirulencemarkersassociatedwithachromobacterspeciesofcfandnoncforigin AT brankojovcic uncoveringdifferencesinvirulencemarkersassociatedwithachromobacterspeciesofcfandnoncforigin AT brankojovcic uncoveringdifferencesinvirulencemarkersassociatedwithachromobacterspeciesofcfandnoncforigin |