Bioactivities of enzymatic protein hydrolysates derived from Chlorella sorokiniana

Abstract Chlorella sorokiniana protein isolates were enzymatically hydrolyzed using pepsin, bromelain, and thermolysin, with their molecular characteristics and bioactivities determined. Thermolysin hydrolysates exhibited the highest degree of hydrolysis (18.08% ± 1.13%). The sodium dodecyl sulfate–...

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Main Authors: Lhumen A. Tejano, Jose P. Peralta, Encarnacion Emilia S. Yap, Yu‐Wei Chang
Format: Article
Language:English
Published: Wiley 2019-07-01
Series:Food Science & Nutrition
Subjects:
Online Access:https://doi.org/10.1002/fsn3.1097
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author Lhumen A. Tejano
Jose P. Peralta
Encarnacion Emilia S. Yap
Yu‐Wei Chang
author_facet Lhumen A. Tejano
Jose P. Peralta
Encarnacion Emilia S. Yap
Yu‐Wei Chang
author_sort Lhumen A. Tejano
collection DOAJ
description Abstract Chlorella sorokiniana protein isolates were enzymatically hydrolyzed using pepsin, bromelain, and thermolysin, with their molecular characteristics and bioactivities determined. Thermolysin hydrolysates exhibited the highest degree of hydrolysis (18.08% ± 1.13%). The sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS‐PAGE) results showed that peptides with molecular weights <10 kDa were found in the hydrolysates compared to the protein isolates. Bioactivity assays revealed that pepsin peptide fraction <5 kDa showed the highest angiotensin‐converting enzyme (ACE)‐inhibitory (34.29% ± 3.45%) and DPPH radical scavenging activities (48.86% ± 1.95%), while pepsin peptide fraction <10 kDa demonstrated the highest reducing power with 0.2101% ± 0.02% absorbance. Moreover, antibacterial assessment revealed that pepsin hydrolysate and peptide fractions displayed inhibition to the test microorganisms. Overall, the present findings suggest that C. sorokiniana protein hydrolysates can be valuable bio‐ingredients with pharmaceutical and nutraceutical application potentials.
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spelling doaj.art-7e9ecd131ee343feb29f08951b2ab8d32024-04-05T09:16:22ZengWileyFood Science & Nutrition2048-71772019-07-01772381239010.1002/fsn3.1097Bioactivities of enzymatic protein hydrolysates derived from Chlorella sorokinianaLhumen A. Tejano0Jose P. Peralta1Encarnacion Emilia S. Yap2Yu‐Wei Chang3College of Fisheries and Ocean Sciences, Institute of Fish Processing Technology University of the Philippines Visayas Miagao Iloilo PhilippinesCollege of Fisheries and Ocean Sciences, Institute of Fish Processing Technology University of the Philippines Visayas Miagao Iloilo PhilippinesCollege of Fisheries and Ocean Sciences, Institute of Fish Processing Technology University of the Philippines Visayas Miagao Iloilo PhilippinesDepartment of Food Science National Taiwan Ocean University Keelung TaiwanAbstract Chlorella sorokiniana protein isolates were enzymatically hydrolyzed using pepsin, bromelain, and thermolysin, with their molecular characteristics and bioactivities determined. Thermolysin hydrolysates exhibited the highest degree of hydrolysis (18.08% ± 1.13%). The sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS‐PAGE) results showed that peptides with molecular weights <10 kDa were found in the hydrolysates compared to the protein isolates. Bioactivity assays revealed that pepsin peptide fraction <5 kDa showed the highest angiotensin‐converting enzyme (ACE)‐inhibitory (34.29% ± 3.45%) and DPPH radical scavenging activities (48.86% ± 1.95%), while pepsin peptide fraction <10 kDa demonstrated the highest reducing power with 0.2101% ± 0.02% absorbance. Moreover, antibacterial assessment revealed that pepsin hydrolysate and peptide fractions displayed inhibition to the test microorganisms. Overall, the present findings suggest that C. sorokiniana protein hydrolysates can be valuable bio‐ingredients with pharmaceutical and nutraceutical application potentials.https://doi.org/10.1002/fsn3.1097angiotensin‐converting enzyme inhibitoryantibacterial activityantioxidant activityChlorella sorokiniana
spellingShingle Lhumen A. Tejano
Jose P. Peralta
Encarnacion Emilia S. Yap
Yu‐Wei Chang
Bioactivities of enzymatic protein hydrolysates derived from Chlorella sorokiniana
Food Science & Nutrition
angiotensin‐converting enzyme inhibitory
antibacterial activity
antioxidant activity
Chlorella sorokiniana
title Bioactivities of enzymatic protein hydrolysates derived from Chlorella sorokiniana
title_full Bioactivities of enzymatic protein hydrolysates derived from Chlorella sorokiniana
title_fullStr Bioactivities of enzymatic protein hydrolysates derived from Chlorella sorokiniana
title_full_unstemmed Bioactivities of enzymatic protein hydrolysates derived from Chlorella sorokiniana
title_short Bioactivities of enzymatic protein hydrolysates derived from Chlorella sorokiniana
title_sort bioactivities of enzymatic protein hydrolysates derived from chlorella sorokiniana
topic angiotensin‐converting enzyme inhibitory
antibacterial activity
antioxidant activity
Chlorella sorokiniana
url https://doi.org/10.1002/fsn3.1097
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AT encarnacionemiliasyap bioactivitiesofenzymaticproteinhydrolysatesderivedfromchlorellasorokiniana
AT yuweichang bioactivitiesofenzymaticproteinhydrolysatesderivedfromchlorellasorokiniana