Changes of Ocular Dimensions as a Marker of Disease Progression in a Murine Model of Pigmentary Glaucoma
PurposeThe elevation of intraocular pressure (IOP), a major risk factor in glaucoma, is an important parameter tracked in experimental models of this disease. However, IOP measurement in laboratory rodents is challenging and may not correlate with some key pathological events that occur in the devel...
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| Format: | Article |
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Frontiers Media S.A.
2020-09-01
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| Series: | Frontiers in Pharmacology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fphar.2020.573238/full |
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| author | Michał Fiedorowicz Michał Fiedorowicz Marlena Wełniak-Kamińska Marlena Wełniak-Kamińska Maciej Świątkiewicz Maciej Świątkiewicz Jarosław Orzeł Jarosław Orzeł Tomasz Chorągiewicz Mario Damiano Toro Mario Damiano Toro Robert Rejdak Piotr Bogorodzki Piotr Bogorodzki Paweł Grieb |
| author_facet | Michał Fiedorowicz Michał Fiedorowicz Marlena Wełniak-Kamińska Marlena Wełniak-Kamińska Maciej Świątkiewicz Maciej Świątkiewicz Jarosław Orzeł Jarosław Orzeł Tomasz Chorągiewicz Mario Damiano Toro Mario Damiano Toro Robert Rejdak Piotr Bogorodzki Piotr Bogorodzki Paweł Grieb |
| author_sort | Michał Fiedorowicz |
| collection | DOAJ |
| description | PurposeThe elevation of intraocular pressure (IOP), a major risk factor in glaucoma, is an important parameter tracked in experimental models of this disease. However, IOP measurement in laboratory rodents is challenging and may not correlate with some key pathological events that occur in the development of glaucoma. The aims of this study were to quantify changes in ocular morphology in DBA/2J mice that develop spontaneous, age-dependent, pigmentary glaucoma and to check the possible correlation of these parameters with IOP.MethodEye morphology was evaluated with MRI in DBA/2J, DBA/2J-Gpnmb+/SjJ, and C57BL/6J female mice ages 3, 6, 9, 12, and 15 months. The animals were anesthetized with isoflurane. A planar receive-only surface coil (inner diameter = 10 mm) was placed over each animal’s left eye and the image was acquired with a 7T small animal-dedicated magnetic resonance tomograph and T2-weighted TurboRARE sequence. Ocular dimensions were manually quantitated using OsiriX software. IOP was measured with rebound tonometry.ResultsIn the control animals, no age-related changes in the ocular morphology were noted. Since 6 months of age, the anterior chamber deepening and elongation of the eyeballs of DBA/2J mice was detectable. We found a significant, positive correlation between IOP and axial length, anterior chamber area, or anterior chamber width in C57BL/6J mice but not in DBA/2J mice. However, after excluding the measurements performed in the oldest DBA/2J mice (i.e. analyzing only the animals ages 3 to 12 months), we demonstrated a significant positive correlation between IOP and anterior chamber width.ConclusionHigh-resolution magnetic resonance imaging of the eye area in mice enables reproducible and consistent measures of key dimensions of the eyeball. We observed age-dependent alterations in the eye morphology of DBA/2J mice that mostly affected the anterior chamber. We also demonstrated a correlation between some of the ocular dimensions and the IOP of C57Bl/6J mice and DBA/2J mice with moderately advanced glaucomatous pathology. |
| first_indexed | 2024-12-20T19:39:41Z |
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| id | doaj.art-7ea33d103f52403a9c3591c8f835b068 |
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| issn | 1663-9812 |
| language | English |
| last_indexed | 2024-12-20T19:39:41Z |
| publishDate | 2020-09-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Pharmacology |
| spelling | doaj.art-7ea33d103f52403a9c3591c8f835b0682022-12-21T19:28:33ZengFrontiers Media S.A.Frontiers in Pharmacology1663-98122020-09-011110.3389/fphar.2020.573238573238Changes of Ocular Dimensions as a Marker of Disease Progression in a Murine Model of Pigmentary GlaucomaMichał Fiedorowicz0Michał Fiedorowicz1Marlena Wełniak-Kamińska2Marlena Wełniak-Kamińska3Maciej Świątkiewicz4Maciej Świątkiewicz5Jarosław Orzeł6Jarosław Orzeł7Tomasz Chorągiewicz8Mario Damiano Toro9Mario Damiano Toro10Robert Rejdak11Piotr Bogorodzki12Piotr Bogorodzki13Paweł Grieb14Department of Experimental Pharmacology, Mossakowski Medical Research Centre, Polish Academy of Sciences, Warsaw, PolandSmall Animal Magnetic Resonance Imaging Laboratory, Mossakowski Medical Research Centre, Polish Academy of Sciences, Warsaw, PolandDepartment of Experimental Pharmacology, Mossakowski Medical Research Centre, Polish Academy of Sciences, Warsaw, PolandSmall Animal Magnetic Resonance Imaging Laboratory, Mossakowski Medical Research Centre, Polish Academy of Sciences, Warsaw, PolandDepartment of Experimental Pharmacology, Mossakowski Medical Research Centre, Polish Academy of Sciences, Warsaw, PolandSmall Animal Magnetic Resonance Imaging Laboratory, Mossakowski Medical Research Centre, Polish Academy of Sciences, Warsaw, PolandSmall Animal Magnetic Resonance Imaging Laboratory, Mossakowski Medical Research Centre, Polish Academy of Sciences, Warsaw, PolandFaculty of Electronics and Information Technology, Warsaw University of Technology, Warsaw, PolandDepartment of General Ophthalmology, Medical University of Lublin, Lublin, PolandDepartment of General Ophthalmology, Medical University of Lublin, Lublin, PolandFaculty of Medical Sciences, Collegium Medicum, Cardinal Stefan Wyszyński University, Warsaw, PolandDepartment of General Ophthalmology, Medical University of Lublin, Lublin, PolandSmall Animal Magnetic Resonance Imaging Laboratory, Mossakowski Medical Research Centre, Polish Academy of Sciences, Warsaw, PolandFaculty of Electronics and Information Technology, Warsaw University of Technology, Warsaw, PolandDepartment of Experimental Pharmacology, Mossakowski Medical Research Centre, Polish Academy of Sciences, Warsaw, PolandPurposeThe elevation of intraocular pressure (IOP), a major risk factor in glaucoma, is an important parameter tracked in experimental models of this disease. However, IOP measurement in laboratory rodents is challenging and may not correlate with some key pathological events that occur in the development of glaucoma. The aims of this study were to quantify changes in ocular morphology in DBA/2J mice that develop spontaneous, age-dependent, pigmentary glaucoma and to check the possible correlation of these parameters with IOP.MethodEye morphology was evaluated with MRI in DBA/2J, DBA/2J-Gpnmb+/SjJ, and C57BL/6J female mice ages 3, 6, 9, 12, and 15 months. The animals were anesthetized with isoflurane. A planar receive-only surface coil (inner diameter = 10 mm) was placed over each animal’s left eye and the image was acquired with a 7T small animal-dedicated magnetic resonance tomograph and T2-weighted TurboRARE sequence. Ocular dimensions were manually quantitated using OsiriX software. IOP was measured with rebound tonometry.ResultsIn the control animals, no age-related changes in the ocular morphology were noted. Since 6 months of age, the anterior chamber deepening and elongation of the eyeballs of DBA/2J mice was detectable. We found a significant, positive correlation between IOP and axial length, anterior chamber area, or anterior chamber width in C57BL/6J mice but not in DBA/2J mice. However, after excluding the measurements performed in the oldest DBA/2J mice (i.e. analyzing only the animals ages 3 to 12 months), we demonstrated a significant positive correlation between IOP and anterior chamber width.ConclusionHigh-resolution magnetic resonance imaging of the eye area in mice enables reproducible and consistent measures of key dimensions of the eyeball. We observed age-dependent alterations in the eye morphology of DBA/2J mice that mostly affected the anterior chamber. We also demonstrated a correlation between some of the ocular dimensions and the IOP of C57Bl/6J mice and DBA/2J mice with moderately advanced glaucomatous pathology.https://www.frontiersin.org/article/10.3389/fphar.2020.573238/fullglaucomaocular dimensionsocular biomechanicsintraocular pressureDBA/2JMRI |
| spellingShingle | Michał Fiedorowicz Michał Fiedorowicz Marlena Wełniak-Kamińska Marlena Wełniak-Kamińska Maciej Świątkiewicz Maciej Świątkiewicz Jarosław Orzeł Jarosław Orzeł Tomasz Chorągiewicz Mario Damiano Toro Mario Damiano Toro Robert Rejdak Piotr Bogorodzki Piotr Bogorodzki Paweł Grieb Changes of Ocular Dimensions as a Marker of Disease Progression in a Murine Model of Pigmentary Glaucoma Frontiers in Pharmacology glaucoma ocular dimensions ocular biomechanics intraocular pressure DBA/2J MRI |
| title | Changes of Ocular Dimensions as a Marker of Disease Progression in a Murine Model of Pigmentary Glaucoma |
| title_full | Changes of Ocular Dimensions as a Marker of Disease Progression in a Murine Model of Pigmentary Glaucoma |
| title_fullStr | Changes of Ocular Dimensions as a Marker of Disease Progression in a Murine Model of Pigmentary Glaucoma |
| title_full_unstemmed | Changes of Ocular Dimensions as a Marker of Disease Progression in a Murine Model of Pigmentary Glaucoma |
| title_short | Changes of Ocular Dimensions as a Marker of Disease Progression in a Murine Model of Pigmentary Glaucoma |
| title_sort | changes of ocular dimensions as a marker of disease progression in a murine model of pigmentary glaucoma |
| topic | glaucoma ocular dimensions ocular biomechanics intraocular pressure DBA/2J MRI |
| url | https://www.frontiersin.org/article/10.3389/fphar.2020.573238/full |
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