Genetic characteristic of class 1 integrons in proteus mirabilis isolates from urine samples
Background: Proteus mirabilis is an opportunistic pathogen, commonly associated with complicated urinary tract infections (UTIs). UTIs caused by multidrug-resistant Proteus mirabilis have increased worldwide. Multidrug-resistance of Gram-negative enteric bacteria is usually associated with class 1 i...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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China Medical University
2017-06-01
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| Series: | BioMedicine |
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| Online Access: | https://doi.org/10.1051/bmdcn/2017070202 |
| _version_ | 1823991230420221952 |
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| author | Chen Chih-Ming Lai Chih-Ho Wu Hwa-Jene Wu Lii-Tzu |
| author_facet | Chen Chih-Ming Lai Chih-Ho Wu Hwa-Jene Wu Lii-Tzu |
| author_sort | Chen Chih-Ming |
| collection | DOAJ |
| description | Background: Proteus mirabilis is an opportunistic pathogen, commonly associated with complicated urinary tract infections (UTIs). UTIs caused by multidrug-resistant Proteus mirabilis have increased worldwide. Multidrug-resistance of Gram-negative enteric bacteria is usually associated with class 1 integrons.
Purposes: To investigate the prevalence and characterize gene cassettes of class 1 integrons in multidrug-resistant P. mirabilis
Methods: From 2006 to 2008, 314 P. mirabilis isolates from urine were collected from a regional teaching hospital. Antimicrobial resistance of the isolates was determined by disk diffusion methods. The phenotypic confirmatory test of extended-spectrum β-lactamase (ESBL) production was performed as described in the Clinical and Laboratory Standards Institute (CLSI) guideline. The genetic organization of the class 1 integron cassettes was investigated by PCR, cloning, and sequencing of the regions surrounding these genes.
Results: Seventy-nine (25%, 79/314) P. mirabilis isolates were ESBL-producing and most ESBL-producing P. mirabilis were positive for blaCTX-M. Class 1 integrons were presented in 76 isolates (24.2%, 76/314), and were more frequently found in ESBL-positive (55/79, 70%) than ESBL-negative (21/235, 8.9%) P. mirabilis isolates. The most prevalence of the cassettes encoded resistance genes were aminoglycoside (aac(6’)-Ib, aacA7, aadAl, aadA2, and aadAla), trimethoprim (dfrAl and dfrA12) and chloramphenicol (catB3 and cmlA6). The most prevalent cassette of dfr12-orfF-aadA2 was found in 49 isolates. The cassette array aadB-catB3-oxa10-aadA1 was first found in P. mirabilis. The enterobacterial repetitive intergenic consensus (ERIC)-PCR fingerprinting patterns were detected in these 76 integron positive P. mirabilis isolates and belonged to 8 profiles.
Conclusion: This study investigated the prevalence and characterized gene cassettes of class 1 integrons in MDR P. mirabilis isolates from urine samples. The frequency of gene cassettes in P. mirabilis were partially by clonal spread of the carriers and the results could provide information for effective antimicrobial therapy and infection control. |
| first_indexed | 2024-12-18T15:51:08Z |
| format | Article |
| id | doaj.art-7ea66496ab484bc9abcb257923abca3a |
| institution | Directory Open Access Journal |
| issn | 2211-8039 |
| language | English |
| last_indexed | 2024-12-18T15:51:08Z |
| publishDate | 2017-06-01 |
| publisher | China Medical University |
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| series | BioMedicine |
| spelling | doaj.art-7ea66496ab484bc9abcb257923abca3a2022-12-21T21:02:36ZengChina Medical UniversityBioMedicine2211-80392017-06-0172910.1051/bmdcn/2017070202bmdcn2017070202Genetic characteristic of class 1 integrons in proteus mirabilis isolates from urine samplesChen Chih-MingLai Chih-HoWu Hwa-JeneWu Lii-TzuBackground: Proteus mirabilis is an opportunistic pathogen, commonly associated with complicated urinary tract infections (UTIs). UTIs caused by multidrug-resistant Proteus mirabilis have increased worldwide. Multidrug-resistance of Gram-negative enteric bacteria is usually associated with class 1 integrons. Purposes: To investigate the prevalence and characterize gene cassettes of class 1 integrons in multidrug-resistant P. mirabilis Methods: From 2006 to 2008, 314 P. mirabilis isolates from urine were collected from a regional teaching hospital. Antimicrobial resistance of the isolates was determined by disk diffusion methods. The phenotypic confirmatory test of extended-spectrum β-lactamase (ESBL) production was performed as described in the Clinical and Laboratory Standards Institute (CLSI) guideline. The genetic organization of the class 1 integron cassettes was investigated by PCR, cloning, and sequencing of the regions surrounding these genes. Results: Seventy-nine (25%, 79/314) P. mirabilis isolates were ESBL-producing and most ESBL-producing P. mirabilis were positive for blaCTX-M. Class 1 integrons were presented in 76 isolates (24.2%, 76/314), and were more frequently found in ESBL-positive (55/79, 70%) than ESBL-negative (21/235, 8.9%) P. mirabilis isolates. The most prevalence of the cassettes encoded resistance genes were aminoglycoside (aac(6’)-Ib, aacA7, aadAl, aadA2, and aadAla), trimethoprim (dfrAl and dfrA12) and chloramphenicol (catB3 and cmlA6). The most prevalent cassette of dfr12-orfF-aadA2 was found in 49 isolates. The cassette array aadB-catB3-oxa10-aadA1 was first found in P. mirabilis. The enterobacterial repetitive intergenic consensus (ERIC)-PCR fingerprinting patterns were detected in these 76 integron positive P. mirabilis isolates and belonged to 8 profiles. Conclusion: This study investigated the prevalence and characterized gene cassettes of class 1 integrons in MDR P. mirabilis isolates from urine samples. The frequency of gene cassettes in P. mirabilis were partially by clonal spread of the carriers and the results could provide information for effective antimicrobial therapy and infection control.https://doi.org/10.1051/bmdcn/2017070202ERICESBLIntegronProteus mirabilis |
| spellingShingle | Chen Chih-Ming Lai Chih-Ho Wu Hwa-Jene Wu Lii-Tzu Genetic characteristic of class 1 integrons in proteus mirabilis isolates from urine samples BioMedicine ERIC ESBL Integron Proteus mirabilis |
| title | Genetic characteristic of class 1 integrons in proteus mirabilis isolates from urine samples |
| title_full | Genetic characteristic of class 1 integrons in proteus mirabilis isolates from urine samples |
| title_fullStr | Genetic characteristic of class 1 integrons in proteus mirabilis isolates from urine samples |
| title_full_unstemmed | Genetic characteristic of class 1 integrons in proteus mirabilis isolates from urine samples |
| title_short | Genetic characteristic of class 1 integrons in proteus mirabilis isolates from urine samples |
| title_sort | genetic characteristic of class 1 integrons in proteus mirabilis isolates from urine samples |
| topic | ERIC ESBL Integron Proteus mirabilis |
| url | https://doi.org/10.1051/bmdcn/2017070202 |
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