Twelve Weeks of High-Intensity Interval Training Alters Adipose Tissue Gene Expression but Not Oxylipin Levels in People with Non-Alcoholic Fatty Liver Disease
Lifestyle modifications, including increased physical activity and exercise, are recommended for non-alcoholic fatty liver disease (NAFLD). Inflamed adipose tissue (AT) contributes to the progression and development of NAFLD and oxylipins such as hydroxyeicosatetraenoic acids (HETE), hydroxydocosahe...
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MDPI AG
2023-05-01
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| Online Access: | https://www.mdpi.com/1422-0067/24/10/8509 |
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| author | Susanne Csader Marsena Jasiel Ismaiah Tiina Kuningas Merja Heinäniemi Janne Suhonen Ville Männistö Heikki Pentikäinen Kai Savonen Milla-Maria Tauriainen Jean-Marie Galano Jetty Chung-Yung Lee Reeta Rintamäki Piia Karisola Hani El-Nezami Ursula Schwab |
| author_facet | Susanne Csader Marsena Jasiel Ismaiah Tiina Kuningas Merja Heinäniemi Janne Suhonen Ville Männistö Heikki Pentikäinen Kai Savonen Milla-Maria Tauriainen Jean-Marie Galano Jetty Chung-Yung Lee Reeta Rintamäki Piia Karisola Hani El-Nezami Ursula Schwab |
| author_sort | Susanne Csader |
| collection | DOAJ |
| description | Lifestyle modifications, including increased physical activity and exercise, are recommended for non-alcoholic fatty liver disease (NAFLD). Inflamed adipose tissue (AT) contributes to the progression and development of NAFLD and oxylipins such as hydroxyeicosatetraenoic acids (HETE), hydroxydocosahexanenoic acids (HDHA), prostaglandins (PEG<sub>2</sub>), and isoprostanoids (IsoP), which all may play a role in AT homeostasis and inflammation. To investigate the role of exercise without weight loss on AT and plasma oxylipin concentrations in NAFLD subjects, we conducted a 12-week randomized controlled exercise intervention. Plasma samples from 39 subjects and abdominal subcutaneous AT biopsy samples from 19 subjects were collected both at the beginning and the end of the exercise intervention. In the AT of women, a significant reduction of gene expression of hemoglobin subunits (<i>HBB</i>, <i>HBA1</i>, <i>HBA2</i>) was observed within the intervention group during the 12-week intervention. Their expression levels were negatively associated with VO<sub>2</sub>max and maxW. In addition, pathways involved in adipocyte morphology alterations significantly increased, whereas pathways in fat metabolism, branched-chain amino acids degradation, and oxidative phosphorylation were suppressed in the intervention group (<i>p</i> < 0.05). Compared to the control group, in the intervention group, the ribosome pathway was activated, but lysosome, oxidative phosphorylation, and pathways of AT modification were suppressed (<i>p</i> < 0.05). Most of the oxylipins (HETE, HDHA, PEG<sub>2</sub>, and IsoP) in plasma did not change during the intervention compared to the control group. 15-F<sub>2t</sub>-IsoP significantly increased in the intervention group compared to the control group (<i>p</i> = 0.014). However, this oxylipin could not be detected in all samples. Exercise intervention without weight loss may influence the AT morphology and fat metabolism at the gene expression level in female NAFLD subjects. |
| first_indexed | 2024-03-11T03:40:38Z |
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| language | English |
| last_indexed | 2024-03-11T03:40:38Z |
| publishDate | 2023-05-01 |
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| spelling | doaj.art-7ea681ef5da6406e83e1228a16f0ab402023-11-18T01:37:02ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-05-012410850910.3390/ijms24108509Twelve Weeks of High-Intensity Interval Training Alters Adipose Tissue Gene Expression but Not Oxylipin Levels in People with Non-Alcoholic Fatty Liver DiseaseSusanne Csader0Marsena Jasiel Ismaiah1Tiina Kuningas2Merja Heinäniemi3Janne Suhonen4Ville Männistö5Heikki Pentikäinen6Kai Savonen7Milla-Maria Tauriainen8Jean-Marie Galano9Jetty Chung-Yung Lee10Reeta Rintamäki11Piia Karisola12Hani El-Nezami13Ursula Schwab14Department of Public Health and Clinical Nutrition, University of Eastern Finland, FI-70200 Kuopio, FinlandSchool of Biological Sciences, The University of Hong Kong, Pokfulam Road, Hong Kong 999077, ChinaInstitute of Biomedicine, School of Medicine, University of Eastern Finland, FI-70210 Kuopio, FinlandInstitute of Biomedicine, School of Medicine, University of Eastern Finland, FI-70210 Kuopio, FinlandInstitute of Biomedicine, School of Medicine, University of Eastern Finland, FI-70210 Kuopio, FinlandDepartment of Medicine, University of Eastern Finland and Kuopio University Hospital, FI-70210 Kuopio, FinlandKuopio Research Institute of Exercise Medicine, FI-70210 Kuopio, FinlandKuopio Research Institute of Exercise Medicine, FI-70210 Kuopio, FinlandDepartment of Public Health and Clinical Nutrition, University of Eastern Finland, FI-70200 Kuopio, FinlandInstitut des Biomolécules Max Mousseron (IBMM), UMR 5247, Université de Montpellier, CNRS, ENSCM, F-34093 Montpellier, FranceSchool of Biological Sciences, The University of Hong Kong, Pokfulam Road, Hong Kong 999077, ChinaDepartment of Medicine, Endocrinology and Clinical Nutrition, Kuopio University Hospital, FI-70210 Kuopio, FinlandFaculty of Medicine, Human Microbiome Research Program, University of Helsinki, FI-00100 Helsinki, FinlandDepartment of Public Health and Clinical Nutrition, University of Eastern Finland, FI-70200 Kuopio, FinlandDepartment of Public Health and Clinical Nutrition, University of Eastern Finland, FI-70200 Kuopio, FinlandLifestyle modifications, including increased physical activity and exercise, are recommended for non-alcoholic fatty liver disease (NAFLD). Inflamed adipose tissue (AT) contributes to the progression and development of NAFLD and oxylipins such as hydroxyeicosatetraenoic acids (HETE), hydroxydocosahexanenoic acids (HDHA), prostaglandins (PEG<sub>2</sub>), and isoprostanoids (IsoP), which all may play a role in AT homeostasis and inflammation. To investigate the role of exercise without weight loss on AT and plasma oxylipin concentrations in NAFLD subjects, we conducted a 12-week randomized controlled exercise intervention. Plasma samples from 39 subjects and abdominal subcutaneous AT biopsy samples from 19 subjects were collected both at the beginning and the end of the exercise intervention. In the AT of women, a significant reduction of gene expression of hemoglobin subunits (<i>HBB</i>, <i>HBA1</i>, <i>HBA2</i>) was observed within the intervention group during the 12-week intervention. Their expression levels were negatively associated with VO<sub>2</sub>max and maxW. In addition, pathways involved in adipocyte morphology alterations significantly increased, whereas pathways in fat metabolism, branched-chain amino acids degradation, and oxidative phosphorylation were suppressed in the intervention group (<i>p</i> < 0.05). Compared to the control group, in the intervention group, the ribosome pathway was activated, but lysosome, oxidative phosphorylation, and pathways of AT modification were suppressed (<i>p</i> < 0.05). Most of the oxylipins (HETE, HDHA, PEG<sub>2</sub>, and IsoP) in plasma did not change during the intervention compared to the control group. 15-F<sub>2t</sub>-IsoP significantly increased in the intervention group compared to the control group (<i>p</i> = 0.014). However, this oxylipin could not be detected in all samples. Exercise intervention without weight loss may influence the AT morphology and fat metabolism at the gene expression level in female NAFLD subjects.https://www.mdpi.com/1422-0067/24/10/8509non-alcoholic fatty liver diseaseadipose tissueexercisegene expressionRNAoxylipin |
| spellingShingle | Susanne Csader Marsena Jasiel Ismaiah Tiina Kuningas Merja Heinäniemi Janne Suhonen Ville Männistö Heikki Pentikäinen Kai Savonen Milla-Maria Tauriainen Jean-Marie Galano Jetty Chung-Yung Lee Reeta Rintamäki Piia Karisola Hani El-Nezami Ursula Schwab Twelve Weeks of High-Intensity Interval Training Alters Adipose Tissue Gene Expression but Not Oxylipin Levels in People with Non-Alcoholic Fatty Liver Disease International Journal of Molecular Sciences non-alcoholic fatty liver disease adipose tissue exercise gene expression RNA oxylipin |
| title | Twelve Weeks of High-Intensity Interval Training Alters Adipose Tissue Gene Expression but Not Oxylipin Levels in People with Non-Alcoholic Fatty Liver Disease |
| title_full | Twelve Weeks of High-Intensity Interval Training Alters Adipose Tissue Gene Expression but Not Oxylipin Levels in People with Non-Alcoholic Fatty Liver Disease |
| title_fullStr | Twelve Weeks of High-Intensity Interval Training Alters Adipose Tissue Gene Expression but Not Oxylipin Levels in People with Non-Alcoholic Fatty Liver Disease |
| title_full_unstemmed | Twelve Weeks of High-Intensity Interval Training Alters Adipose Tissue Gene Expression but Not Oxylipin Levels in People with Non-Alcoholic Fatty Liver Disease |
| title_short | Twelve Weeks of High-Intensity Interval Training Alters Adipose Tissue Gene Expression but Not Oxylipin Levels in People with Non-Alcoholic Fatty Liver Disease |
| title_sort | twelve weeks of high intensity interval training alters adipose tissue gene expression but not oxylipin levels in people with non alcoholic fatty liver disease |
| topic | non-alcoholic fatty liver disease adipose tissue exercise gene expression RNA oxylipin |
| url | https://www.mdpi.com/1422-0067/24/10/8509 |
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