Miltefosine induces programmed cell death in Leishmania amazonensis promastigotes
In the current study, we evaluated the mechanism of action of miltefosine, which is the first effective and safe oral treatment for visceral leishmaniasis, in Leishmania amazonensis promastigotes. Miltefosine induced a process of programmed cell death, which was determined by the externalization of...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Fundação Oswaldo Cruz (FIOCRUZ)
2011-06-01
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| Series: | Memorias do Instituto Oswaldo Cruz |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762011000400021&lng=en&tlng=en |
| _version_ | 1797721280844136448 |
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| author | Fernanda de Aquino Marinho Keyla Cristiny da Silva Gonçalves Selma Soares de Oliveira Ana-Carolina de Siqueira Couto de Oliveira Maria Bellio Claudia Masini d'Avila-Levy André Luis Souza dos Santos Marta Helena Branquinha |
| author_facet | Fernanda de Aquino Marinho Keyla Cristiny da Silva Gonçalves Selma Soares de Oliveira Ana-Carolina de Siqueira Couto de Oliveira Maria Bellio Claudia Masini d'Avila-Levy André Luis Souza dos Santos Marta Helena Branquinha |
| author_sort | Fernanda de Aquino Marinho |
| collection | DOAJ |
| description | In the current study, we evaluated the mechanism of action of miltefosine, which is the first effective and safe oral treatment for visceral leishmaniasis, in Leishmania amazonensis promastigotes. Miltefosine induced a process of programmed cell death, which was determined by the externalization of phosphatidylserine, the incorporation of propidium iodide, cell-cycle arrest at the sub-G0/G1 phase and DNA fragmentation into oligonucleosome-sized fragments. Despite the intrinsic variation that is detected in Leishmania spp, our results indicate that miltefosine causes apoptosis-like death in L. amazonensis promastigote cells using a similar process that is observed in Leishmania donovani. |
| first_indexed | 2024-03-12T09:31:16Z |
| format | Article |
| id | doaj.art-7ea7c6c2b000485ea86bf96039562185 |
| institution | Directory Open Access Journal |
| issn | 1678-8060 |
| language | English |
| last_indexed | 2024-03-12T09:31:16Z |
| publishDate | 2011-06-01 |
| publisher | Fundação Oswaldo Cruz (FIOCRUZ) |
| record_format | Article |
| series | Memorias do Instituto Oswaldo Cruz |
| spelling | doaj.art-7ea7c6c2b000485ea86bf960395621852023-09-02T13:54:10ZengFundação Oswaldo Cruz (FIOCRUZ)Memorias do Instituto Oswaldo Cruz1678-80602011-06-01106450750910.1590/S0074-02762011000400021S0074-02762011000400021Miltefosine induces programmed cell death in Leishmania amazonensis promastigotesFernanda de Aquino Marinho0Keyla Cristiny da Silva Gonçalves1Selma Soares de Oliveira2Ana-Carolina de Siqueira Couto de Oliveira3Maria Bellio4Claudia Masini d'Avila-Levy5André Luis Souza dos Santos6Marta Helena Branquinha7Universidade Federal do Rio de JaneiroUniversidade Federal do Rio de JaneiroUniversidade Federal do Rio de JaneiroUniversidade Federal do Rio de JaneiroUniversidade Federal do Rio de JaneiroFundação Oswaldo CruzUniversidade Federal do Rio de JaneiroUniversidade Federal do Rio de JaneiroIn the current study, we evaluated the mechanism of action of miltefosine, which is the first effective and safe oral treatment for visceral leishmaniasis, in Leishmania amazonensis promastigotes. Miltefosine induced a process of programmed cell death, which was determined by the externalization of phosphatidylserine, the incorporation of propidium iodide, cell-cycle arrest at the sub-G0/G1 phase and DNA fragmentation into oligonucleosome-sized fragments. Despite the intrinsic variation that is detected in Leishmania spp, our results indicate that miltefosine causes apoptosis-like death in L. amazonensis promastigote cells using a similar process that is observed in Leishmania donovani.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762011000400021&lng=en&tlng=enleishmaniasischemotherapyapoptosis |
| spellingShingle | Fernanda de Aquino Marinho Keyla Cristiny da Silva Gonçalves Selma Soares de Oliveira Ana-Carolina de Siqueira Couto de Oliveira Maria Bellio Claudia Masini d'Avila-Levy André Luis Souza dos Santos Marta Helena Branquinha Miltefosine induces programmed cell death in Leishmania amazonensis promastigotes Memorias do Instituto Oswaldo Cruz leishmaniasis chemotherapy apoptosis |
| title | Miltefosine induces programmed cell death in Leishmania amazonensis promastigotes |
| title_full | Miltefosine induces programmed cell death in Leishmania amazonensis promastigotes |
| title_fullStr | Miltefosine induces programmed cell death in Leishmania amazonensis promastigotes |
| title_full_unstemmed | Miltefosine induces programmed cell death in Leishmania amazonensis promastigotes |
| title_short | Miltefosine induces programmed cell death in Leishmania amazonensis promastigotes |
| title_sort | miltefosine induces programmed cell death in leishmania amazonensis promastigotes |
| topic | leishmaniasis chemotherapy apoptosis |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762011000400021&lng=en&tlng=en |
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