Cytogenetic identification and molecular marker development of a novel wheat-Leymus mollis 4Ns(4D) alien disomic substitution line with resistance to stripe rust and Fusarium head blight
Leymus mollis (Trin.) Pilg. (2n = 4x = 28, NsNsXmXm) potentially harbours useful genes that might contribute to the improvement of wheat. We describe M862 as a novel wheat-L. mollis alien disomic substitution line from a cross between wheat cv. 7182 and octoploid Tritileymus M47. Cytological observa...
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Frontiers Media S.A.
2022-11-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2022.1012939/full |
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| author | Xin Du Xin Du Xin Du Xianbo Feng Xianbo Feng Xianbo Feng Ruoxuan Li Ruoxuan Li Ruoxuan Li Yanlong Jin Yanlong Jin Lihui Shang Lihui Shang Lihui Shang Jixin Zhao Jixin Zhao Jixin Zhao Changyou Wang Changyou Wang Changyou Wang Tingdong Li Tingdong Li Tingdong Li Chunhuan Chen Chunhuan Chen Zengrong Tian Zengrong Tian Zengrong Tian Pingchuan Deng Pingchuan Deng Pingchuan Deng Wanquan Ji Wanquan Ji Wanquan Ji |
| author_facet | Xin Du Xin Du Xin Du Xianbo Feng Xianbo Feng Xianbo Feng Ruoxuan Li Ruoxuan Li Ruoxuan Li Yanlong Jin Yanlong Jin Lihui Shang Lihui Shang Lihui Shang Jixin Zhao Jixin Zhao Jixin Zhao Changyou Wang Changyou Wang Changyou Wang Tingdong Li Tingdong Li Tingdong Li Chunhuan Chen Chunhuan Chen Zengrong Tian Zengrong Tian Zengrong Tian Pingchuan Deng Pingchuan Deng Pingchuan Deng Wanquan Ji Wanquan Ji Wanquan Ji |
| author_sort | Xin Du |
| collection | DOAJ |
| description | Leymus mollis (Trin.) Pilg. (2n = 4x = 28, NsNsXmXm) potentially harbours useful genes that might contribute to the improvement of wheat. We describe M862 as a novel wheat-L. mollis alien disomic substitution line from a cross between wheat cv. 7182 and octoploid Tritileymus M47. Cytological observations indicate that M862 has a chromosome constitution of 2n = 42 = 21II. Two 4D chromosomes of wheat substituted by two L. mollis Ns chromosomes were observed, using the GISH and ND-FISH analyses. Molecular marker, 55K SNP array and wheat-P. huashanica liquid array (GenoBaits®WheatplusPh) analyses further indicate that the alien chromosomes are L. mollis 4Ns. Therefore, it was deduced that M862 was a wheat-L. mollis 4Ns(4D) alien disomic substitution line. There were also changes in chromosomes 1A, 1D, 2B and 5A detected by ND-FISH analysis. Transcriptome sequencing showed that the structural variation of 1D, 1A and 5A may have smaller impact on gene expression than that for 2B. In addition, a total of 16 markers derived from Lm#4Ns were developed from transcriptome sequences, and these proved to be highly effective for tracking the introduced chromosome. M862 showed reduced height, larger grains (weight and width), and was highly resistance to CYR32 and CYR34 stripe rust races at the seedling stage and mixed stripe rust races (CYR32, CYR33 and CYR34) at the adult stage. It was also resistance to Fusarium head blight (FHB). This alien disomic substitution line M862 may be exploited as an important genetic material in the domestication of stipe rust and FHB resistance wheat varieties. |
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| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-13T16:51:17Z |
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| spelling | doaj.art-7ea87a912551459bb569b5b3dfb3c38c2022-12-22T02:38:56ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2022-11-011310.3389/fpls.2022.10129391012939Cytogenetic identification and molecular marker development of a novel wheat-Leymus mollis 4Ns(4D) alien disomic substitution line with resistance to stripe rust and Fusarium head blightXin Du0Xin Du1Xin Du2Xianbo Feng3Xianbo Feng4Xianbo Feng5Ruoxuan Li6Ruoxuan Li7Ruoxuan Li8Yanlong Jin9Yanlong Jin10Lihui Shang11Lihui Shang12Lihui Shang13Jixin Zhao14Jixin Zhao15Jixin Zhao16Changyou Wang17Changyou Wang18Changyou Wang19Tingdong Li20Tingdong Li21Tingdong Li22Chunhuan Chen23Chunhuan Chen24Zengrong Tian25Zengrong Tian26Zengrong Tian27Pingchuan Deng28Pingchuan Deng29Pingchuan Deng30Wanquan Ji31Wanquan Ji32Wanquan Ji33College of Agronomy, Northwest A & F University, Yangling, Shaanxi, ChinaState Key Laboratory of Crop Stress Biology for Arid Areas, Northwest A & F University, Yangling, Shaanxi, ChinaShaanxi Research Station of Crop Gene Resources and Germplasm Enhancement, Ministry of Agriculture, Yangling, Shaanxi, ChinaCollege of Agronomy, Northwest A & F University, Yangling, Shaanxi, ChinaState Key Laboratory of Crop Stress Biology for Arid Areas, Northwest A & F University, Yangling, Shaanxi, ChinaShaanxi Research Station of Crop Gene Resources and Germplasm Enhancement, Ministry of Agriculture, Yangling, Shaanxi, ChinaCollege of Agronomy, Northwest A & F University, Yangling, Shaanxi, ChinaState Key Laboratory of Crop Stress Biology for Arid Areas, Northwest A & F University, Yangling, Shaanxi, ChinaShaanxi Research Station of Crop Gene Resources and Germplasm Enhancement, Ministry of Agriculture, Yangling, Shaanxi, ChinaCollege of Agronomy, Northwest A & F University, Yangling, Shaanxi, ChinaInstitute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, ChinaCollege of Agronomy, Northwest A & F University, Yangling, Shaanxi, ChinaState Key Laboratory of Crop Stress Biology for Arid Areas, Northwest A & F University, Yangling, Shaanxi, ChinaShaanxi Research Station of Crop Gene Resources and Germplasm Enhancement, Ministry of Agriculture, Yangling, Shaanxi, ChinaCollege of Agronomy, Northwest A & F University, Yangling, Shaanxi, ChinaState Key Laboratory of Crop Stress Biology for Arid Areas, Northwest A & F University, Yangling, Shaanxi, ChinaShaanxi Research Station of Crop Gene Resources and Germplasm Enhancement, Ministry of Agriculture, Yangling, Shaanxi, ChinaCollege of Agronomy, Northwest A & F University, Yangling, Shaanxi, ChinaState Key Laboratory of Crop Stress Biology for Arid Areas, Northwest A & F University, Yangling, Shaanxi, ChinaShaanxi Research Station of Crop Gene Resources and Germplasm Enhancement, Ministry of Agriculture, Yangling, Shaanxi, ChinaCollege of Agronomy, Northwest A & F University, Yangling, Shaanxi, ChinaState Key Laboratory of Crop Stress Biology for Arid Areas, Northwest A & F University, Yangling, Shaanxi, ChinaShaanxi Research Station of Crop Gene Resources and Germplasm Enhancement, Ministry of Agriculture, Yangling, Shaanxi, ChinaCollege of Agronomy, Northwest A & F University, Yangling, Shaanxi, ChinaShaanxi Research Station of Crop Gene Resources and Germplasm Enhancement, Ministry of Agriculture, Yangling, Shaanxi, ChinaCollege of Agronomy, Northwest A & F University, Yangling, Shaanxi, ChinaState Key Laboratory of Crop Stress Biology for Arid Areas, Northwest A & F University, Yangling, Shaanxi, ChinaShaanxi Research Station of Crop Gene Resources and Germplasm Enhancement, Ministry of Agriculture, Yangling, Shaanxi, ChinaCollege of Agronomy, Northwest A & F University, Yangling, Shaanxi, ChinaState Key Laboratory of Crop Stress Biology for Arid Areas, Northwest A & F University, Yangling, Shaanxi, ChinaShaanxi Research Station of Crop Gene Resources and Germplasm Enhancement, Ministry of Agriculture, Yangling, Shaanxi, ChinaCollege of Agronomy, Northwest A & F University, Yangling, Shaanxi, ChinaState Key Laboratory of Crop Stress Biology for Arid Areas, Northwest A & F University, Yangling, Shaanxi, ChinaShaanxi Research Station of Crop Gene Resources and Germplasm Enhancement, Ministry of Agriculture, Yangling, Shaanxi, ChinaLeymus mollis (Trin.) Pilg. (2n = 4x = 28, NsNsXmXm) potentially harbours useful genes that might contribute to the improvement of wheat. We describe M862 as a novel wheat-L. mollis alien disomic substitution line from a cross between wheat cv. 7182 and octoploid Tritileymus M47. Cytological observations indicate that M862 has a chromosome constitution of 2n = 42 = 21II. Two 4D chromosomes of wheat substituted by two L. mollis Ns chromosomes were observed, using the GISH and ND-FISH analyses. Molecular marker, 55K SNP array and wheat-P. huashanica liquid array (GenoBaits®WheatplusPh) analyses further indicate that the alien chromosomes are L. mollis 4Ns. Therefore, it was deduced that M862 was a wheat-L. mollis 4Ns(4D) alien disomic substitution line. There were also changes in chromosomes 1A, 1D, 2B and 5A detected by ND-FISH analysis. Transcriptome sequencing showed that the structural variation of 1D, 1A and 5A may have smaller impact on gene expression than that for 2B. In addition, a total of 16 markers derived from Lm#4Ns were developed from transcriptome sequences, and these proved to be highly effective for tracking the introduced chromosome. M862 showed reduced height, larger grains (weight and width), and was highly resistance to CYR32 and CYR34 stripe rust races at the seedling stage and mixed stripe rust races (CYR32, CYR33 and CYR34) at the adult stage. It was also resistance to Fusarium head blight (FHB). This alien disomic substitution line M862 may be exploited as an important genetic material in the domestication of stipe rust and FHB resistance wheat varieties.https://www.frontiersin.org/articles/10.3389/fpls.2022.1012939/fullLeymus mollisalien disomic substitution linestripe rustFHBtranscriptome sequencingliquid array |
| spellingShingle | Xin Du Xin Du Xin Du Xianbo Feng Xianbo Feng Xianbo Feng Ruoxuan Li Ruoxuan Li Ruoxuan Li Yanlong Jin Yanlong Jin Lihui Shang Lihui Shang Lihui Shang Jixin Zhao Jixin Zhao Jixin Zhao Changyou Wang Changyou Wang Changyou Wang Tingdong Li Tingdong Li Tingdong Li Chunhuan Chen Chunhuan Chen Zengrong Tian Zengrong Tian Zengrong Tian Pingchuan Deng Pingchuan Deng Pingchuan Deng Wanquan Ji Wanquan Ji Wanquan Ji Cytogenetic identification and molecular marker development of a novel wheat-Leymus mollis 4Ns(4D) alien disomic substitution line with resistance to stripe rust and Fusarium head blight Frontiers in Plant Science Leymus mollis alien disomic substitution line stripe rust FHB transcriptome sequencing liquid array |
| title | Cytogenetic identification and molecular marker development of a novel wheat-Leymus mollis 4Ns(4D) alien disomic substitution line with resistance to stripe rust and Fusarium head blight |
| title_full | Cytogenetic identification and molecular marker development of a novel wheat-Leymus mollis 4Ns(4D) alien disomic substitution line with resistance to stripe rust and Fusarium head blight |
| title_fullStr | Cytogenetic identification and molecular marker development of a novel wheat-Leymus mollis 4Ns(4D) alien disomic substitution line with resistance to stripe rust and Fusarium head blight |
| title_full_unstemmed | Cytogenetic identification and molecular marker development of a novel wheat-Leymus mollis 4Ns(4D) alien disomic substitution line with resistance to stripe rust and Fusarium head blight |
| title_short | Cytogenetic identification and molecular marker development of a novel wheat-Leymus mollis 4Ns(4D) alien disomic substitution line with resistance to stripe rust and Fusarium head blight |
| title_sort | cytogenetic identification and molecular marker development of a novel wheat leymus mollis 4ns 4d alien disomic substitution line with resistance to stripe rust and fusarium head blight |
| topic | Leymus mollis alien disomic substitution line stripe rust FHB transcriptome sequencing liquid array |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2022.1012939/full |
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