Bovine Herpesvirus-1 antibodies levels and associated Risk Factors in unvaccinated Dairy Herds from tropical wet weather, Ecuador
In order to determine the antibodies levels against Bovine Herpesvirus type 1 (BHV-1) and associated risk factors in unvaccinated dairy herds of tropical wet weather of Ecuador, an investigation was carried out in thirteen production units (PU), in the Chone Canton, Province of Manabí. The sample s...
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| Format: | Article |
| Language: | English |
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Universidad del Zulia
2022-05-01
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| Series: | Revista Científica |
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| Online Access: | https://produccioncientificaluz.org/index.php/cientifica/article/view/38094 |
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| author | Hitler Cedeño-Sánchez Brinner Burgos-García Jhoana Zambrano-Aveiga Marcia Jurado-Hidalgo Patricia Zambrano-Moreira María Lugo-Almarza María Gabriela Farías Francisco Angulo-Cubillán |
| author_facet | Hitler Cedeño-Sánchez Brinner Burgos-García Jhoana Zambrano-Aveiga Marcia Jurado-Hidalgo Patricia Zambrano-Moreira María Lugo-Almarza María Gabriela Farías Francisco Angulo-Cubillán |
| author_sort | Hitler Cedeño-Sánchez |
| collection | DOAJ |
| description |
In order to determine the antibodies levels against Bovine Herpesvirus type 1 (BHV-1) and associated risk factors in unvaccinated dairy herds of tropical wet weather of Ecuador, an investigation was carried out in thirteen production units (PU), in the Chone Canton, Province of Manabí. The sample size was calculate by cluster sampling and the variables to be evaluated were antibodies levels frequency, age, sex, ocular or vulvovaginal lesions, breed and PU. One hundred eighty three blood samples were taken from the jugular or caudal vein and labeled for transfer to the laboratory. The tubes were centrifuged at 3,220 g for 15 minutes and the sera were transferred to Eppendorf tubes that once labeled were stored at –20°C until processing. Antibodies levels against HBV-1 were measured through of competitive ELISA technique, and calculated with simples statistical. The risk factors were estimated through the Odds ratio and relative risk, and the significance level through the Chi-square. The general antibodies levels frequency was 58.47 %. According age, 28.57 % in animals under two years old (yr.old) and 67.38 % for cattle over two yr.old. According to the sex, males showed 18.18 % and females 82.61 %. The determined risk factors (P<0.05) were age and sex, with a significant relative risk (P<0.05) of 3.31 and 2.32, respectively. The unvaccinated dairy herds of Western lowlands of Ecuador, have high antibodies levels frequency against BHV-1, with females over two yr.old at the highest risk of presenting infectious bovine rhinotracheitis (IBR).
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| first_indexed | 2024-04-10T06:47:22Z |
| format | Article |
| id | doaj.art-7ea9373615d4471ba3b8f640ad280399 |
| institution | Directory Open Access Journal |
| issn | 0798-2259 2521-9715 |
| language | English |
| last_indexed | 2024-04-10T06:47:22Z |
| publishDate | 2022-05-01 |
| publisher | Universidad del Zulia |
| record_format | Article |
| series | Revista Científica |
| spelling | doaj.art-7ea9373615d4471ba3b8f640ad2803992023-02-28T12:27:31ZengUniversidad del ZuliaRevista Científica0798-22592521-97152022-05-013210.52973/rcfcv-e32088Bovine Herpesvirus-1 antibodies levels and associated Risk Factors in unvaccinated Dairy Herds from tropical wet weather, EcuadorHitler Cedeño-Sánchez0Brinner Burgos-García1Jhoana Zambrano-Aveiga2Marcia Jurado-Hidalgo3Patricia Zambrano-Moreira4María Lugo-Almarza5María Gabriela Farías6Francisco Angulo-Cubillán7Universidad Laica Eloy Alfaro de Manabí, Extensión Chone, carrera de Ingeniería Agropecuaria. Chone, Manabí, EcuadorUniversidad Laica Eloy Alfaro de Manabí, Extensión Chone, carrera de Ingeniería Agropecuaria. Chone, Manabí, EcuadorUniversidad Laica Eloy Alfaro de Manabí, Extensión Chone, carrera de Ingeniería Agropecuaria. Chone, Manabí, EcuadorUniversidad Laica Eloy Alfaro de Manabí, Extensión Chone, carrera de Ingeniería Agropecuaria. Chone, Manabí, EcuadorUniversidad Laica Eloy Alfaro de Manabí, Extensión Chone, carrera de Ingeniería Agropecuaria. Chone, Manabí, EcuadorClínica Veterinaria Bulldog. Santo Domingo, Santo Domingo de los Tsáchilas, EcuadorUniversidad Laica Eloy Alfaro de Manabí, Extensión Chone, carrera de Ingeniería Agropecuaria. Chone, Manabí, EcuadorUniversidad UTE, Sede Santo Domingo, Facultad de Ciencias de la Salud Eugenio Espejo, carrera de Medicina Veterinaria, Veterinary Science Research Group. Santo Domingo, Santo Domingo de los Tsáchilas, Ecuador - Universidad del Zulia, Facultad de Ciencias Veterinarias, Departamento de Sanidad Animal. Maracaibo, Zulia, Venezuela In order to determine the antibodies levels against Bovine Herpesvirus type 1 (BHV-1) and associated risk factors in unvaccinated dairy herds of tropical wet weather of Ecuador, an investigation was carried out in thirteen production units (PU), in the Chone Canton, Province of Manabí. The sample size was calculate by cluster sampling and the variables to be evaluated were antibodies levels frequency, age, sex, ocular or vulvovaginal lesions, breed and PU. One hundred eighty three blood samples were taken from the jugular or caudal vein and labeled for transfer to the laboratory. The tubes were centrifuged at 3,220 g for 15 minutes and the sera were transferred to Eppendorf tubes that once labeled were stored at –20°C until processing. Antibodies levels against HBV-1 were measured through of competitive ELISA technique, and calculated with simples statistical. The risk factors were estimated through the Odds ratio and relative risk, and the significance level through the Chi-square. The general antibodies levels frequency was 58.47 %. According age, 28.57 % in animals under two years old (yr.old) and 67.38 % for cattle over two yr.old. According to the sex, males showed 18.18 % and females 82.61 %. The determined risk factors (P<0.05) were age and sex, with a significant relative risk (P<0.05) of 3.31 and 2.32, respectively. The unvaccinated dairy herds of Western lowlands of Ecuador, have high antibodies levels frequency against BHV-1, with females over two yr.old at the highest risk of presenting infectious bovine rhinotracheitis (IBR). https://produccioncientificaluz.org/index.php/cientifica/article/view/38094Dairy herdsBHV-1seroprevalencerisk factorsunvaccinated |
| spellingShingle | Hitler Cedeño-Sánchez Brinner Burgos-García Jhoana Zambrano-Aveiga Marcia Jurado-Hidalgo Patricia Zambrano-Moreira María Lugo-Almarza María Gabriela Farías Francisco Angulo-Cubillán Bovine Herpesvirus-1 antibodies levels and associated Risk Factors in unvaccinated Dairy Herds from tropical wet weather, Ecuador Revista Científica Dairy herds BHV-1 seroprevalence risk factors unvaccinated |
| title | Bovine Herpesvirus-1 antibodies levels and associated Risk Factors in unvaccinated Dairy Herds from tropical wet weather, Ecuador |
| title_full | Bovine Herpesvirus-1 antibodies levels and associated Risk Factors in unvaccinated Dairy Herds from tropical wet weather, Ecuador |
| title_fullStr | Bovine Herpesvirus-1 antibodies levels and associated Risk Factors in unvaccinated Dairy Herds from tropical wet weather, Ecuador |
| title_full_unstemmed | Bovine Herpesvirus-1 antibodies levels and associated Risk Factors in unvaccinated Dairy Herds from tropical wet weather, Ecuador |
| title_short | Bovine Herpesvirus-1 antibodies levels and associated Risk Factors in unvaccinated Dairy Herds from tropical wet weather, Ecuador |
| title_sort | bovine herpesvirus 1 antibodies levels and associated risk factors in unvaccinated dairy herds from tropical wet weather ecuador |
| topic | Dairy herds BHV-1 seroprevalence risk factors unvaccinated |
| url | https://produccioncientificaluz.org/index.php/cientifica/article/view/38094 |
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