Therapeutic drug monitoring of six contraindicated/co-administered drugs by simple and green RP-HPLC-PDA; application to spiked human plasma
Abstract Therapeutic drug monitoring is an important clinical testing of the drugs to monitor their concentrations in plasma in order to guarantee their optimal impact, and to avoid any side effects resulting from drug-drug interactions. A green reversed-phase high-performance liquid chromatographic...
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| Format: | Article |
| Language: | English |
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BMC
2024-04-01
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| Series: | BMC Chemistry |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s13065-024-01161-y |
| _version_ | 1797220072888991744 |
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| author | Nada Hesham Maha A. Hegazy Hebatallah A. Wagdy |
| author_facet | Nada Hesham Maha A. Hegazy Hebatallah A. Wagdy |
| author_sort | Nada Hesham |
| collection | DOAJ |
| description | Abstract Therapeutic drug monitoring is an important clinical testing of the drugs to monitor their concentrations in plasma in order to guarantee their optimal impact, and to avoid any side effects resulting from drug-drug interactions. A green reversed-phase high-performance liquid chromatographic method using a photodiode array detector (RP-HPLC-PDA) was developed for the simultaneous determination of three carbapenem antibiotics (Imipenem, ertapenem, and meropenem) with the co-formulated drug (cilastatin) and contraindicated drugs (probenecid and warfarin) in spiked human plasma. The separation was achieved at 25 °C using a gradient elution of a mixture of mobile phase A: methanol and mobile phase B: phosphate buffer (pH 3.0). The photodiode array detector was adjusted at 220 nm. Bioanalytical method validation was carried out as per the FDA guidelines, and the method showed good linearity ranges for the six drugs that included their Cmax levels along with low limits of quantification. Based on the results, the method was found to be accurate and precise; with high % recovery and good % RSD, respectively. The method was successfully applied to spiked human plasma, signifying a good potential to be implemented in future TDM studies of these drugs when co-administered together. |
| first_indexed | 2024-04-24T12:43:44Z |
| format | Article |
| id | doaj.art-7eaaca743db443a9bfbe63a43d249d15 |
| institution | Directory Open Access Journal |
| issn | 2661-801X |
| language | English |
| last_indexed | 2024-04-24T12:43:44Z |
| publishDate | 2024-04-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Chemistry |
| spelling | doaj.art-7eaaca743db443a9bfbe63a43d249d152024-04-07T11:07:17ZengBMCBMC Chemistry2661-801X2024-04-0118111410.1186/s13065-024-01161-yTherapeutic drug monitoring of six contraindicated/co-administered drugs by simple and green RP-HPLC-PDA; application to spiked human plasmaNada Hesham0Maha A. Hegazy1Hebatallah A. Wagdy2Pharmaceutical Chemistry Department, Faculty of Pharmacy, The British University in Egypt (BUE)Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Cairo UniversityPharmaceutical Chemistry Department, Faculty of Pharmacy, The British University in Egypt (BUE)Abstract Therapeutic drug monitoring is an important clinical testing of the drugs to monitor their concentrations in plasma in order to guarantee their optimal impact, and to avoid any side effects resulting from drug-drug interactions. A green reversed-phase high-performance liquid chromatographic method using a photodiode array detector (RP-HPLC-PDA) was developed for the simultaneous determination of three carbapenem antibiotics (Imipenem, ertapenem, and meropenem) with the co-formulated drug (cilastatin) and contraindicated drugs (probenecid and warfarin) in spiked human plasma. The separation was achieved at 25 °C using a gradient elution of a mixture of mobile phase A: methanol and mobile phase B: phosphate buffer (pH 3.0). The photodiode array detector was adjusted at 220 nm. Bioanalytical method validation was carried out as per the FDA guidelines, and the method showed good linearity ranges for the six drugs that included their Cmax levels along with low limits of quantification. Based on the results, the method was found to be accurate and precise; with high % recovery and good % RSD, respectively. The method was successfully applied to spiked human plasma, signifying a good potential to be implemented in future TDM studies of these drugs when co-administered together.https://doi.org/10.1186/s13065-024-01161-yChromatographic analysisBioanalyticalTherapeutic drug monitoringPharmacovigilanceImipenemMeropenem |
| spellingShingle | Nada Hesham Maha A. Hegazy Hebatallah A. Wagdy Therapeutic drug monitoring of six contraindicated/co-administered drugs by simple and green RP-HPLC-PDA; application to spiked human plasma BMC Chemistry Chromatographic analysis Bioanalytical Therapeutic drug monitoring Pharmacovigilance Imipenem Meropenem |
| title | Therapeutic drug monitoring of six contraindicated/co-administered drugs by simple and green RP-HPLC-PDA; application to spiked human plasma |
| title_full | Therapeutic drug monitoring of six contraindicated/co-administered drugs by simple and green RP-HPLC-PDA; application to spiked human plasma |
| title_fullStr | Therapeutic drug monitoring of six contraindicated/co-administered drugs by simple and green RP-HPLC-PDA; application to spiked human plasma |
| title_full_unstemmed | Therapeutic drug monitoring of six contraindicated/co-administered drugs by simple and green RP-HPLC-PDA; application to spiked human plasma |
| title_short | Therapeutic drug monitoring of six contraindicated/co-administered drugs by simple and green RP-HPLC-PDA; application to spiked human plasma |
| title_sort | therapeutic drug monitoring of six contraindicated co administered drugs by simple and green rp hplc pda application to spiked human plasma |
| topic | Chromatographic analysis Bioanalytical Therapeutic drug monitoring Pharmacovigilance Imipenem Meropenem |
| url | https://doi.org/10.1186/s13065-024-01161-y |
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