Mammary core clock gene expression is impacted by photoperiod exposure during the dry period in goats
Short-day photoperiod (SDPP) during the dry period increases milk production compared to long-day photoperiod (LDPP) in goats. Photoperiod information is sent to the master clock in the suprachiasmatic nuclei (SCN), which send temporal information to peripheral clocks located in every tissue of the...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2018-01-01
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| Series: | Journal of Applied Animal Research |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/09712119.2018.1486317 |
| _version_ | 1830491923814547456 |
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| author | Theresa M. Casey Karen Plaut Mugagga Kalyesubula Avi Shamay Chris Sabastian Yosi Wein Enav Bar-Shira Naama Reicher Sameer. J. Mabjeesh |
| author_facet | Theresa M. Casey Karen Plaut Mugagga Kalyesubula Avi Shamay Chris Sabastian Yosi Wein Enav Bar-Shira Naama Reicher Sameer. J. Mabjeesh |
| author_sort | Theresa M. Casey |
| collection | DOAJ |
| description | Short-day photoperiod (SDPP) during the dry period increases milk production compared to long-day photoperiod (LDPP) in goats. Photoperiod information is sent to the master clock in the suprachiasmatic nuclei (SCN), which send temporal information to peripheral clocks located in every tissue of the body. We hypothesized photoperiod effects on milk production are mediated in part by changes in mammary clocks. Our objective was to determine the effect of photoperiod manipulation during the dry period in goats on core clock genes expression in mammary gland. Multiparous goats (n = 6) were blocked at dry off into two treatments: LDPP and SDPP. Serial mammary biopsies were taken over a 24 h period during three weeks prepartum. Total RNA was isolated, and q-PCR analysis of the core clock genes CLOCK, ARNTL, PER1, CRY1, and CRY2 found exposure to LDPP significantly increased ARNTL (P < .05) expression relative to SDPP exposure. Plasma prolactin levels were significantly increased (P < .05) after seven weeks of photoperiod treatment, but not at three weeks prepartum. The mechanism and significance of photoperiod-induced changes in mammary clock also need to be elucidated but may be related to seasonal changes in lactation. |
| first_indexed | 2024-12-21T20:20:49Z |
| format | Article |
| id | doaj.art-7ead3f50789e434c9e90b0b0ca5f157b |
| institution | Directory Open Access Journal |
| issn | 0971-2119 0974-1844 |
| language | English |
| last_indexed | 2024-12-21T20:20:49Z |
| publishDate | 2018-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Journal of Applied Animal Research |
| spelling | doaj.art-7ead3f50789e434c9e90b0b0ca5f157b2022-12-21T18:51:30ZengTaylor & Francis GroupJournal of Applied Animal Research0971-21190974-18442018-01-014611214121910.1080/09712119.2018.14863171486317Mammary core clock gene expression is impacted by photoperiod exposure during the dry period in goatsTheresa M. Casey0Karen Plaut1Mugagga Kalyesubula2Avi Shamay3Chris Sabastian4Yosi Wein5Enav Bar-Shira6Naama Reicher7Sameer. J. Mabjeesh8Purdue UniversityPurdue UniversityFood and Environment The Hebrew UniversityThe Volcani CenterFood and Environment The Hebrew UniversityFood and Environment The Hebrew UniversityFood and Environment The Hebrew UniversityFood and Environment The Hebrew UniversityFood and Environment The Hebrew UniversityShort-day photoperiod (SDPP) during the dry period increases milk production compared to long-day photoperiod (LDPP) in goats. Photoperiod information is sent to the master clock in the suprachiasmatic nuclei (SCN), which send temporal information to peripheral clocks located in every tissue of the body. We hypothesized photoperiod effects on milk production are mediated in part by changes in mammary clocks. Our objective was to determine the effect of photoperiod manipulation during the dry period in goats on core clock genes expression in mammary gland. Multiparous goats (n = 6) were blocked at dry off into two treatments: LDPP and SDPP. Serial mammary biopsies were taken over a 24 h period during three weeks prepartum. Total RNA was isolated, and q-PCR analysis of the core clock genes CLOCK, ARNTL, PER1, CRY1, and CRY2 found exposure to LDPP significantly increased ARNTL (P < .05) expression relative to SDPP exposure. Plasma prolactin levels were significantly increased (P < .05) after seven weeks of photoperiod treatment, but not at three weeks prepartum. The mechanism and significance of photoperiod-induced changes in mammary clock also need to be elucidated but may be related to seasonal changes in lactation.http://dx.doi.org/10.1080/09712119.2018.1486317Photoperioddry periodgoatmammarycircadian clock |
| spellingShingle | Theresa M. Casey Karen Plaut Mugagga Kalyesubula Avi Shamay Chris Sabastian Yosi Wein Enav Bar-Shira Naama Reicher Sameer. J. Mabjeesh Mammary core clock gene expression is impacted by photoperiod exposure during the dry period in goats Journal of Applied Animal Research Photoperiod dry period goat mammary circadian clock |
| title | Mammary core clock gene expression is impacted by photoperiod exposure during the dry period in goats |
| title_full | Mammary core clock gene expression is impacted by photoperiod exposure during the dry period in goats |
| title_fullStr | Mammary core clock gene expression is impacted by photoperiod exposure during the dry period in goats |
| title_full_unstemmed | Mammary core clock gene expression is impacted by photoperiod exposure during the dry period in goats |
| title_short | Mammary core clock gene expression is impacted by photoperiod exposure during the dry period in goats |
| title_sort | mammary core clock gene expression is impacted by photoperiod exposure during the dry period in goats |
| topic | Photoperiod dry period goat mammary circadian clock |
| url | http://dx.doi.org/10.1080/09712119.2018.1486317 |
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