Prevalence of colistin-resistant mcr-1-positive Escherichia coli isolated from children patients with diarrhoea in Shanghai, 2016–2021
ABSTRACT: Objectives: The emergence of the plasmid-mediated colistin resistance 1 (mcr-1) of Escherichia coli has become a global health concern. This study reports the prevalence of mcr-1 among E. coli isolates from patients with diarrheal disease in Shanghai and the genetic characterization of mc...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-09-01
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| Series: | Journal of Global Antimicrobial Resistance |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213716523000966 |
| _version_ | 1797689506161229824 |
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| author | Jun Feng Yuan Zhuang Jiayuan Luo Quan Xiao Yitong Wu Yong Chen Min Chen Xi Zhang |
| author_facet | Jun Feng Yuan Zhuang Jiayuan Luo Quan Xiao Yitong Wu Yong Chen Min Chen Xi Zhang |
| author_sort | Jun Feng |
| collection | DOAJ |
| description | ABSTRACT: Objectives: The emergence of the plasmid-mediated colistin resistance 1 (mcr-1) of Escherichia coli has become a global health concern. This study reports the prevalence of mcr-1 among E. coli isolates from patients with diarrheal disease in Shanghai and the genetic characterization of mcr-1-harbouring plasmids. Methods: A total of 1723 E. coli strains were collected from the faeces of patients with diarrheal disease in all sentinel hospitals in Shanghai from 2016 to 2021. Antimicrobial susceptibility testing was performed by broth microdilution and plasmid conjunction transfer assay was carried out using E. coli C600 as the recipient. The mcr-1-positive E. coli strains (MCRPEC) were subjected to molecular characterization and bioinformatic analysis of the mcr-1-bearing plasmids that they harboured. Results: Only 5 (0.28%) strains were found to harbour the mcr-1 gene using PCR screening. Plasmid conjugation assay and whole-genome sequencing indicated that EC16500, one MCRPEC strain that co-exhibited mcr-1, blaTEM-1, blaOXA-1, qnrS1, qnrS2, arr-3, and catB3, could be conjugated to EC C600 by horizontal transfer with an average efficiency of 3.2 × 10−5. The plasmid pEC16500 harboured similar backbones as p70_2_15, pECGD-8–33, pNCYU-29–19–1_MCR1, and pIBMC_mcr1, and was shown to be encoded within a type IV secretion system (T4SS)-containing 32.6 kbp IncX4, next to the pap2-like membrane-associated gene, to form a 2.4-kb cassette. Furthermore, sequencing and phylogenetic analyses revealed a similarity between other MCR-1-homolog proteins, indicating that the five E. coli isolates were colistin-resistant. Conclusion: Our data represents a significant snapshot of colistin resistance mcr-1 genes and highlights the need to increase active surveillance, especially among children under five years of age, in Shanghai. Great effort needs to be taken to avoid further dissemination of plasmid-mediated colistin resistance among clinically relevant Gram-negative bacterial pathogens. |
| first_indexed | 2024-03-12T01:46:32Z |
| format | Article |
| id | doaj.art-7eae4dfdfae44caea6a98d2c973a6a9c |
| institution | Directory Open Access Journal |
| issn | 2213-7165 |
| language | English |
| last_indexed | 2024-03-12T01:46:32Z |
| publishDate | 2023-09-01 |
| publisher | Elsevier |
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| series | Journal of Global Antimicrobial Resistance |
| spelling | doaj.art-7eae4dfdfae44caea6a98d2c973a6a9c2023-09-09T04:55:10ZengElsevierJournal of Global Antimicrobial Resistance2213-71652023-09-0134166175Prevalence of colistin-resistant mcr-1-positive Escherichia coli isolated from children patients with diarrhoea in Shanghai, 2016–2021Jun Feng0Yuan Zhuang1Jiayuan Luo2Quan Xiao3Yitong Wu4Yong Chen5Min Chen6Xi Zhang7Shanghai Municipal Center for Disease Control and Prevention, Shanghai, People's Republic of ChinaShanghai Municipal Center for Disease Control and Prevention, Shanghai, People's Republic of ChinaShanghai Municipal Center for Disease Control and Prevention, Shanghai, People's Republic of ChinaShanghai Municipal Center for Disease Control and Prevention, Shanghai, People's Republic of ChinaShanghai Municipal Center for Disease Control and Prevention, Shanghai, People's Republic of ChinaShanghai Municipal Center for Disease Control and Prevention, Shanghai, People's Republic of ChinaCorresponding authors.; Shanghai Municipal Center for Disease Control and Prevention, Shanghai, People's Republic of ChinaCorresponding authors.; Shanghai Municipal Center for Disease Control and Prevention, Shanghai, People's Republic of ChinaABSTRACT: Objectives: The emergence of the plasmid-mediated colistin resistance 1 (mcr-1) of Escherichia coli has become a global health concern. This study reports the prevalence of mcr-1 among E. coli isolates from patients with diarrheal disease in Shanghai and the genetic characterization of mcr-1-harbouring plasmids. Methods: A total of 1723 E. coli strains were collected from the faeces of patients with diarrheal disease in all sentinel hospitals in Shanghai from 2016 to 2021. Antimicrobial susceptibility testing was performed by broth microdilution and plasmid conjunction transfer assay was carried out using E. coli C600 as the recipient. The mcr-1-positive E. coli strains (MCRPEC) were subjected to molecular characterization and bioinformatic analysis of the mcr-1-bearing plasmids that they harboured. Results: Only 5 (0.28%) strains were found to harbour the mcr-1 gene using PCR screening. Plasmid conjugation assay and whole-genome sequencing indicated that EC16500, one MCRPEC strain that co-exhibited mcr-1, blaTEM-1, blaOXA-1, qnrS1, qnrS2, arr-3, and catB3, could be conjugated to EC C600 by horizontal transfer with an average efficiency of 3.2 × 10−5. The plasmid pEC16500 harboured similar backbones as p70_2_15, pECGD-8–33, pNCYU-29–19–1_MCR1, and pIBMC_mcr1, and was shown to be encoded within a type IV secretion system (T4SS)-containing 32.6 kbp IncX4, next to the pap2-like membrane-associated gene, to form a 2.4-kb cassette. Furthermore, sequencing and phylogenetic analyses revealed a similarity between other MCR-1-homolog proteins, indicating that the five E. coli isolates were colistin-resistant. Conclusion: Our data represents a significant snapshot of colistin resistance mcr-1 genes and highlights the need to increase active surveillance, especially among children under five years of age, in Shanghai. Great effort needs to be taken to avoid further dissemination of plasmid-mediated colistin resistance among clinically relevant Gram-negative bacterial pathogens.http://www.sciencedirect.com/science/article/pii/S2213716523000966MCR-1Escherichia coliAntimicrobial susceptibilityIncX4Conjugation experimentsType IV secretion system |
| spellingShingle | Jun Feng Yuan Zhuang Jiayuan Luo Quan Xiao Yitong Wu Yong Chen Min Chen Xi Zhang Prevalence of colistin-resistant mcr-1-positive Escherichia coli isolated from children patients with diarrhoea in Shanghai, 2016–2021 Journal of Global Antimicrobial Resistance MCR-1 Escherichia coli Antimicrobial susceptibility IncX4 Conjugation experiments Type IV secretion system |
| title | Prevalence of colistin-resistant mcr-1-positive Escherichia coli isolated from children patients with diarrhoea in Shanghai, 2016–2021 |
| title_full | Prevalence of colistin-resistant mcr-1-positive Escherichia coli isolated from children patients with diarrhoea in Shanghai, 2016–2021 |
| title_fullStr | Prevalence of colistin-resistant mcr-1-positive Escherichia coli isolated from children patients with diarrhoea in Shanghai, 2016–2021 |
| title_full_unstemmed | Prevalence of colistin-resistant mcr-1-positive Escherichia coli isolated from children patients with diarrhoea in Shanghai, 2016–2021 |
| title_short | Prevalence of colistin-resistant mcr-1-positive Escherichia coli isolated from children patients with diarrhoea in Shanghai, 2016–2021 |
| title_sort | prevalence of colistin resistant mcr 1 positive escherichia coli isolated from children patients with diarrhoea in shanghai 2016 2021 |
| topic | MCR-1 Escherichia coli Antimicrobial susceptibility IncX4 Conjugation experiments Type IV secretion system |
| url | http://www.sciencedirect.com/science/article/pii/S2213716523000966 |
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