Derhamnosylmaysin Inhibits Adipogenesis via Inhibiting Expression of PPARγ and C/EBPα in 3T3-L1 Cells
We investigated the effects of derhamnosylmaysin (DM) on adipogenesis and lipid accumulation in 3T3-L1 adipocytes. Our data showed that DM inhibited lipid accumulation and adipocyte differentiation in 3T3-L1 cells. Treatment of 3T3-L1 adipocytes with DM decreased the expression of major transcriptio...
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MDPI AG
2022-06-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/27/13/4232 |
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| author | Hang-Hee Cho Sun-Hee Jang Chungkil Won Chung-Hui Kim Hong-Duck Kim Tae Hoon Kim Jae-Hyeon Cho |
| author_facet | Hang-Hee Cho Sun-Hee Jang Chungkil Won Chung-Hui Kim Hong-Duck Kim Tae Hoon Kim Jae-Hyeon Cho |
| author_sort | Hang-Hee Cho |
| collection | DOAJ |
| description | We investigated the effects of derhamnosylmaysin (DM) on adipogenesis and lipid accumulation in 3T3-L1 adipocytes. Our data showed that DM inhibited lipid accumulation and adipocyte differentiation in 3T3-L1 cells. Treatment of 3T3-L1 adipocytes with DM decreased the expression of major transcription factors, such as sterol regulatory element-binding protein-1c (SREBP-1c), the CCAAT-enhancer-binding protein (CEBP) family, and peroxisome proliferator-activated receptor gamma (PPARγ), in the regulation of adipocyte differentiation. Moreover, the expression of their downstream target genes related to adipogenesis and lipogenesis, including adipocyte fatty acid-binding protein (aP2), lipoprotein lipase (LPL), stearyl-CoA-desaturase-1 (SCD-1), acetyl-CoA carboxylase (ACC), and fatty acid synthase (FAS), was also decreased by treatment with DM during adipogenesis. Additionally, DM attenuated insulin-stimulated phosphorylation of Akt. These results first demonstrated that DM inhibited adipogenesis and lipogenesis through downregulation of the key adipogenic transcription factors SREBP-1c, the CEBP family, and PPARγ and inactivation of the major adipogenesis signaling factor Akt, which is intermediated in insulin. These studies demonstrated that DM is a new bioactive compound for antiadipogenic reagents for controlling overweight and obesity. |
| first_indexed | 2024-03-09T04:00:31Z |
| format | Article |
| id | doaj.art-7eae657ef711475a9ca682e4173cebf8 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-09T04:00:31Z |
| publishDate | 2022-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-7eae657ef711475a9ca682e4173cebf82023-12-03T14:13:58ZengMDPI AGMolecules1420-30492022-06-012713423210.3390/molecules27134232Derhamnosylmaysin Inhibits Adipogenesis via Inhibiting Expression of PPARγ and C/EBPα in 3T3-L1 CellsHang-Hee Cho0Sun-Hee Jang1Chungkil Won2Chung-Hui Kim3Hong-Duck Kim4Tae Hoon Kim5Jae-Hyeon Cho6Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University, Jinju 52828, KoreaInstitute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University, Jinju 52828, KoreaInstitute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University, Jinju 52828, KoreaInstitute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University, Jinju 52828, KoreaDepartment of Public Health, Division of Environmental Health Science, New York Medical College, Valhalla, NY 10595, USADepartment of Food Science and Biotechnology, Daegu University, Gyungsan 38453, KoreaInstitute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University, Jinju 52828, KoreaWe investigated the effects of derhamnosylmaysin (DM) on adipogenesis and lipid accumulation in 3T3-L1 adipocytes. Our data showed that DM inhibited lipid accumulation and adipocyte differentiation in 3T3-L1 cells. Treatment of 3T3-L1 adipocytes with DM decreased the expression of major transcription factors, such as sterol regulatory element-binding protein-1c (SREBP-1c), the CCAAT-enhancer-binding protein (CEBP) family, and peroxisome proliferator-activated receptor gamma (PPARγ), in the regulation of adipocyte differentiation. Moreover, the expression of their downstream target genes related to adipogenesis and lipogenesis, including adipocyte fatty acid-binding protein (aP2), lipoprotein lipase (LPL), stearyl-CoA-desaturase-1 (SCD-1), acetyl-CoA carboxylase (ACC), and fatty acid synthase (FAS), was also decreased by treatment with DM during adipogenesis. Additionally, DM attenuated insulin-stimulated phosphorylation of Akt. These results first demonstrated that DM inhibited adipogenesis and lipogenesis through downregulation of the key adipogenic transcription factors SREBP-1c, the CEBP family, and PPARγ and inactivation of the major adipogenesis signaling factor Akt, which is intermediated in insulin. These studies demonstrated that DM is a new bioactive compound for antiadipogenic reagents for controlling overweight and obesity.https://www.mdpi.com/1420-3049/27/13/4232adipogenesisAktderhamnosylmaysinlipogenesis3T3-L1 cells |
| spellingShingle | Hang-Hee Cho Sun-Hee Jang Chungkil Won Chung-Hui Kim Hong-Duck Kim Tae Hoon Kim Jae-Hyeon Cho Derhamnosylmaysin Inhibits Adipogenesis via Inhibiting Expression of PPARγ and C/EBPα in 3T3-L1 Cells Molecules adipogenesis Akt derhamnosylmaysin lipogenesis 3T3-L1 cells |
| title | Derhamnosylmaysin Inhibits Adipogenesis via Inhibiting Expression of PPARγ and C/EBPα in 3T3-L1 Cells |
| title_full | Derhamnosylmaysin Inhibits Adipogenesis via Inhibiting Expression of PPARγ and C/EBPα in 3T3-L1 Cells |
| title_fullStr | Derhamnosylmaysin Inhibits Adipogenesis via Inhibiting Expression of PPARγ and C/EBPα in 3T3-L1 Cells |
| title_full_unstemmed | Derhamnosylmaysin Inhibits Adipogenesis via Inhibiting Expression of PPARγ and C/EBPα in 3T3-L1 Cells |
| title_short | Derhamnosylmaysin Inhibits Adipogenesis via Inhibiting Expression of PPARγ and C/EBPα in 3T3-L1 Cells |
| title_sort | derhamnosylmaysin inhibits adipogenesis via inhibiting expression of pparγ and c ebpα in 3t3 l1 cells |
| topic | adipogenesis Akt derhamnosylmaysin lipogenesis 3T3-L1 cells |
| url | https://www.mdpi.com/1420-3049/27/13/4232 |
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