Glucose Detection of 4-Mercaptophenylboronic Acid-Immobilized Gold-Silver Core-Shell Assembled Silica Nanostructure by Surface Enhanced Raman Scattering
The importance of glucose in many biological processes continues to garner increasing research interest in the design and development of efficient biotechnology for the sensitive and selective monitoring of glucose. Here we report on a surface-enhanced Raman scattering (SERS) detection of 4-mercapto...
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MDPI AG
2021-04-01
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| Series: | Nanomaterials |
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| Online Access: | https://www.mdpi.com/2079-4991/11/4/948 |
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| author | Xuan-Hung Pham Bomi Seong Eunil Hahm Kim-Hung Huynh Yoon-Hee Kim Jaehi Kim Sang Hun Lee Bong-Hyun Jun |
| author_facet | Xuan-Hung Pham Bomi Seong Eunil Hahm Kim-Hung Huynh Yoon-Hee Kim Jaehi Kim Sang Hun Lee Bong-Hyun Jun |
| author_sort | Xuan-Hung Pham |
| collection | DOAJ |
| description | The importance of glucose in many biological processes continues to garner increasing research interest in the design and development of efficient biotechnology for the sensitive and selective monitoring of glucose. Here we report on a surface-enhanced Raman scattering (SERS) detection of 4-mercaptophenyl boronic acid (4-MPBA)-immobilized gold-silver core-shell assembled silica nanostructure (SiO<sub>2</sub>@Au@Ag@4-MPBA) for quantitative, selective detection of glucose in physiologically relevant concentration. This work confirmed that 4-MPBA converted to 4-mercaptophenol (4-MPhOH) in the presence of H<sub>2</sub>O<sub>2</sub>. In addition, a calibration curve for H<sub>2</sub>O<sub>2</sub> detection of 0.3 µg/mL was successfully detected in the range of 1.0 to 1000 µg/mL. Moreover, the SiO<sub>2</sub>@Au@Ag@4-MPBA for glucose detection was developed in the presence of glucose oxidase (GOx) at the optimized condition of 100 µg/mL GOx with 1-h incubation time using 20 µg/mL SiO<sub>2</sub>@Au@Ag@4-MPBA and measuring Raman signal at 67 µg/mL SiO<sub>2</sub>@Au@Ag. At the optimized condition, the calibration curve in the range of 0.5 to 8.0 mM was successfully developed with an LOD of 0.15 mM. Based on those strategies, the SERS detection of glucose can be achieved in the physiologically relevant concentration range and opened a great promise to develop a SERS-based biosensor for a variety of biomedicine applications. |
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| language | English |
| last_indexed | 2024-03-10T12:30:59Z |
| publishDate | 2021-04-01 |
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| series | Nanomaterials |
| spelling | doaj.art-7eaf72a9fa5442e8906f8cfe9730af0a2023-11-21T14:41:28ZengMDPI AGNanomaterials2079-49912021-04-0111494810.3390/nano11040948Glucose Detection of 4-Mercaptophenylboronic Acid-Immobilized Gold-Silver Core-Shell Assembled Silica Nanostructure by Surface Enhanced Raman ScatteringXuan-Hung Pham0Bomi Seong1Eunil Hahm2Kim-Hung Huynh3Yoon-Hee Kim4Jaehi Kim5Sang Hun Lee6Bong-Hyun Jun7Department of Bioscience and Biotechnology, Konkuk University, Seoul 143-701, KoreaDepartment of Bioscience and Biotechnology, Konkuk University, Seoul 143-701, KoreaDepartment of Bioscience and Biotechnology, Konkuk University, Seoul 143-701, KoreaDepartment of Bioscience and Biotechnology, Konkuk University, Seoul 143-701, KoreaDepartment of Bioscience and Biotechnology, Konkuk University, Seoul 143-701, KoreaDepartment of Bioscience and Biotechnology, Konkuk University, Seoul 143-701, KoreaDepartment of Chemical and Biological Engineering, Hanbat National University, Daejeon 34158, KoreaDepartment of Bioscience and Biotechnology, Konkuk University, Seoul 143-701, KoreaThe importance of glucose in many biological processes continues to garner increasing research interest in the design and development of efficient biotechnology for the sensitive and selective monitoring of glucose. Here we report on a surface-enhanced Raman scattering (SERS) detection of 4-mercaptophenyl boronic acid (4-MPBA)-immobilized gold-silver core-shell assembled silica nanostructure (SiO<sub>2</sub>@Au@Ag@4-MPBA) for quantitative, selective detection of glucose in physiologically relevant concentration. This work confirmed that 4-MPBA converted to 4-mercaptophenol (4-MPhOH) in the presence of H<sub>2</sub>O<sub>2</sub>. In addition, a calibration curve for H<sub>2</sub>O<sub>2</sub> detection of 0.3 µg/mL was successfully detected in the range of 1.0 to 1000 µg/mL. Moreover, the SiO<sub>2</sub>@Au@Ag@4-MPBA for glucose detection was developed in the presence of glucose oxidase (GOx) at the optimized condition of 100 µg/mL GOx with 1-h incubation time using 20 µg/mL SiO<sub>2</sub>@Au@Ag@4-MPBA and measuring Raman signal at 67 µg/mL SiO<sub>2</sub>@Au@Ag. At the optimized condition, the calibration curve in the range of 0.5 to 8.0 mM was successfully developed with an LOD of 0.15 mM. Based on those strategies, the SERS detection of glucose can be achieved in the physiologically relevant concentration range and opened a great promise to develop a SERS-based biosensor for a variety of biomedicine applications.https://www.mdpi.com/2079-4991/11/4/948surface enhanced Raman scatteringgold-silver core-shellgold-silver core-shell assembled silica nanostructurehydrogen peroxideglucose detection4-mercaptophenylboronic acid |
| spellingShingle | Xuan-Hung Pham Bomi Seong Eunil Hahm Kim-Hung Huynh Yoon-Hee Kim Jaehi Kim Sang Hun Lee Bong-Hyun Jun Glucose Detection of 4-Mercaptophenylboronic Acid-Immobilized Gold-Silver Core-Shell Assembled Silica Nanostructure by Surface Enhanced Raman Scattering Nanomaterials surface enhanced Raman scattering gold-silver core-shell gold-silver core-shell assembled silica nanostructure hydrogen peroxide glucose detection 4-mercaptophenylboronic acid |
| title | Glucose Detection of 4-Mercaptophenylboronic Acid-Immobilized Gold-Silver Core-Shell Assembled Silica Nanostructure by Surface Enhanced Raman Scattering |
| title_full | Glucose Detection of 4-Mercaptophenylboronic Acid-Immobilized Gold-Silver Core-Shell Assembled Silica Nanostructure by Surface Enhanced Raman Scattering |
| title_fullStr | Glucose Detection of 4-Mercaptophenylboronic Acid-Immobilized Gold-Silver Core-Shell Assembled Silica Nanostructure by Surface Enhanced Raman Scattering |
| title_full_unstemmed | Glucose Detection of 4-Mercaptophenylboronic Acid-Immobilized Gold-Silver Core-Shell Assembled Silica Nanostructure by Surface Enhanced Raman Scattering |
| title_short | Glucose Detection of 4-Mercaptophenylboronic Acid-Immobilized Gold-Silver Core-Shell Assembled Silica Nanostructure by Surface Enhanced Raman Scattering |
| title_sort | glucose detection of 4 mercaptophenylboronic acid immobilized gold silver core shell assembled silica nanostructure by surface enhanced raman scattering |
| topic | surface enhanced Raman scattering gold-silver core-shell gold-silver core-shell assembled silica nanostructure hydrogen peroxide glucose detection 4-mercaptophenylboronic acid |
| url | https://www.mdpi.com/2079-4991/11/4/948 |
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