Sleep restriction prior to antigen exposure does not alter the T cell receptor repertoire but impairs germinal center formation during a T cell-dependent B cell response in murine spleen
It is well known that sleep promotes immune functions. In line with this, a variety of studies in animal models and humans have shown that sleep restriction following an antigen challenge dampens the immune response on several levels which leads to e.g. worsening of disease outcome and reduction of...
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| Format: | Article |
| Language: | English |
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Elsevier
2021-10-01
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| Series: | Brain, Behavior, & Immunity - Health |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666354621001150 |
| _version_ | 1818574676801093632 |
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| author | Cornelia Tune Julia Hahn Stella E. Autenrieth Martin Meinhardt Rene Pagel Andrea Schampel Lisa-Kristin Schierloh Kathrin Kalies Juergen Westermann |
| author_facet | Cornelia Tune Julia Hahn Stella E. Autenrieth Martin Meinhardt Rene Pagel Andrea Schampel Lisa-Kristin Schierloh Kathrin Kalies Juergen Westermann |
| author_sort | Cornelia Tune |
| collection | DOAJ |
| description | It is well known that sleep promotes immune functions. In line with this, a variety of studies in animal models and humans have shown that sleep restriction following an antigen challenge dampens the immune response on several levels which leads to e.g. worsening of disease outcome and reduction of vaccination efficiency, respectively. However, the inverse scenario with sleep restriction preceding an antigen challenge is only investigated in a few animal models where it has been shown to reduce antigen uptake and presentation as well as pathogen clearance and survival rates. Here, we use injection of sheep red blood cells to investigate the yet unknown effect on a T cell-dependent B cell response in a well-established mouse model. We found that 6 h of sleep restriction prior to the antigen challenge does not impact the T cell reaction including the T cell receptor repertoire but dampens the development of germinal centers which correlates with reduced antigen-specific antibody titer indicating an impaired B cell response. These changes concerned a functionally more relevant level than those found in the same experimental model with the inverse scenario when sleep restriction followed the antigen challenge. Taken together, our findings showed that the outcome of the T cell-dependent B cell response is indeed impacted by sleep restriction prior to the antigen challenge which highlights the clinical significance of this scenario and the need for further investigations in humans, for example concerning the effect of sleep restriction preceding a vaccination. |
| first_indexed | 2024-12-15T00:29:43Z |
| format | Article |
| id | doaj.art-7eb12f02553b48fe83269b5d29f85e4a |
| institution | Directory Open Access Journal |
| issn | 2666-3546 |
| language | English |
| last_indexed | 2024-12-15T00:29:43Z |
| publishDate | 2021-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Brain, Behavior, & Immunity - Health |
| spelling | doaj.art-7eb12f02553b48fe83269b5d29f85e4a2022-12-21T22:42:04ZengElsevierBrain, Behavior, & Immunity - Health2666-35462021-10-0116100312Sleep restriction prior to antigen exposure does not alter the T cell receptor repertoire but impairs germinal center formation during a T cell-dependent B cell response in murine spleenCornelia Tune0Julia Hahn1Stella E. Autenrieth2Martin Meinhardt3Rene Pagel4Andrea Schampel5Lisa-Kristin Schierloh6Kathrin Kalies7Juergen Westermann8Institute of Anatomy, University of Luebeck, GermanyDepartment of Internal Medicine II, University of Tuebingen, GermanyDepartment of Internal Medicine II, University of Tuebingen, GermanyInstitute of Anatomy, University of Luebeck, GermanyInstitute of Anatomy, University of Luebeck, GermanyInstitute of Anatomy, University of Luebeck, GermanyInstitute of Anatomy, University of Luebeck, GermanyInstitute of Anatomy, University of Luebeck, GermanyInstitute of Anatomy, University of Luebeck, Germany; Corresponding author. Institute for Anatomy, University of Luebeck, Ratzeburger Allee 160, 23562, Luebeck, Germany.It is well known that sleep promotes immune functions. In line with this, a variety of studies in animal models and humans have shown that sleep restriction following an antigen challenge dampens the immune response on several levels which leads to e.g. worsening of disease outcome and reduction of vaccination efficiency, respectively. However, the inverse scenario with sleep restriction preceding an antigen challenge is only investigated in a few animal models where it has been shown to reduce antigen uptake and presentation as well as pathogen clearance and survival rates. Here, we use injection of sheep red blood cells to investigate the yet unknown effect on a T cell-dependent B cell response in a well-established mouse model. We found that 6 h of sleep restriction prior to the antigen challenge does not impact the T cell reaction including the T cell receptor repertoire but dampens the development of germinal centers which correlates with reduced antigen-specific antibody titer indicating an impaired B cell response. These changes concerned a functionally more relevant level than those found in the same experimental model with the inverse scenario when sleep restriction followed the antigen challenge. Taken together, our findings showed that the outcome of the T cell-dependent B cell response is indeed impacted by sleep restriction prior to the antigen challenge which highlights the clinical significance of this scenario and the need for further investigations in humans, for example concerning the effect of sleep restriction preceding a vaccination.http://www.sciencedirect.com/science/article/pii/S2666354621001150Sleep deprivationAntigen presentationT cell-dependent B cell responseSheep red blood cellsGerminal centerMouse |
| spellingShingle | Cornelia Tune Julia Hahn Stella E. Autenrieth Martin Meinhardt Rene Pagel Andrea Schampel Lisa-Kristin Schierloh Kathrin Kalies Juergen Westermann Sleep restriction prior to antigen exposure does not alter the T cell receptor repertoire but impairs germinal center formation during a T cell-dependent B cell response in murine spleen Brain, Behavior, & Immunity - Health Sleep deprivation Antigen presentation T cell-dependent B cell response Sheep red blood cells Germinal center Mouse |
| title | Sleep restriction prior to antigen exposure does not alter the T cell receptor repertoire but impairs germinal center formation during a T cell-dependent B cell response in murine spleen |
| title_full | Sleep restriction prior to antigen exposure does not alter the T cell receptor repertoire but impairs germinal center formation during a T cell-dependent B cell response in murine spleen |
| title_fullStr | Sleep restriction prior to antigen exposure does not alter the T cell receptor repertoire but impairs germinal center formation during a T cell-dependent B cell response in murine spleen |
| title_full_unstemmed | Sleep restriction prior to antigen exposure does not alter the T cell receptor repertoire but impairs germinal center formation during a T cell-dependent B cell response in murine spleen |
| title_short | Sleep restriction prior to antigen exposure does not alter the T cell receptor repertoire but impairs germinal center formation during a T cell-dependent B cell response in murine spleen |
| title_sort | sleep restriction prior to antigen exposure does not alter the t cell receptor repertoire but impairs germinal center formation during a t cell dependent b cell response in murine spleen |
| topic | Sleep deprivation Antigen presentation T cell-dependent B cell response Sheep red blood cells Germinal center Mouse |
| url | http://www.sciencedirect.com/science/article/pii/S2666354621001150 |
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