Anti-Melanogenesis Effect of Polysaccharide from <i>Saussurea involucrata</i> on Forskolin-Induced Melanogenesis in B16F10 Melanoma Cells
As one of the prominent medicinal plants listed in the Chinese pharmacopoeia (2020), <i>Saussurea involucrata</i> (Kar. et Kir.) Sch.-Bip was demonstrated to possess various therapeutic effects. In our recent research, we extracted the polysaccharides from <i>S. involucrata</i&g...
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MDPI AG
2022-11-01
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| author | Mayila Kamilijiang Deng Zang Nuermaimaiti Abudukelimu Nurbolat Aidarhan Geyu Liu Haji Akber Aisa |
| author_facet | Mayila Kamilijiang Deng Zang Nuermaimaiti Abudukelimu Nurbolat Aidarhan Geyu Liu Haji Akber Aisa |
| author_sort | Mayila Kamilijiang |
| collection | DOAJ |
| description | As one of the prominent medicinal plants listed in the Chinese pharmacopoeia (2020), <i>Saussurea involucrata</i> (Kar. et Kir.) Sch.-Bip was demonstrated to possess various therapeutic effects. In our recent research, we extracted the polysaccharides from <i>S. involucrata</i> (SIP) at optimal conditions and conducted further structure elucidation on the main fraction as well as the confirmation of its possible anti-inflammatory activity. Hence, in this work, we assessed the in vitro antioxidant activity and anti-melanogenesis effects of the crude SIP in forskolin-induced B16F10 melanoma cells. The results show that SIP possessed strong antioxidant activity and was effective in concentration-dependently decreasing melanin formation and inhibiting tyrosinase activity in forskolin-induced B16F10 cells. Based on these results, the inhibitory mechanism of melanogenesis was investigated by measuring Tyrosinase (TYR), Tyrosinase related protein-1 (TRP-1), Tyrosinase related protein-2 (TRP-2), Microphthalmia-associated transcription factor (MITF), cAMP-response element binding protein (CREB), mitogen-activated protein kinases (MAPK) signaling protein members, and β-catenin degradation in forskolin-induced B16F10 cells. The anti-melanogenesis response of SIP might be attributed to the regulation of c-Jun <i>N</i>-terminal kinase (JNK) phosphorylation and β-catenin degradation pathways. These results suggest that polysaccharides from <i>S. involucrata</i> possess a strong anti-melanogenic effect, and thus could be used as a high-value natural material for skin whitening in cosmeceutical industries. |
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| spelling | doaj.art-7eb27f237e3b4d1db25b28a7a6891e362023-11-24T11:51:13ZengMDPI AGNutrients2072-66432022-11-011423504410.3390/nu14235044Anti-Melanogenesis Effect of Polysaccharide from <i>Saussurea involucrata</i> on Forskolin-Induced Melanogenesis in B16F10 Melanoma CellsMayila Kamilijiang0Deng Zang1Nuermaimaiti Abudukelimu2Nurbolat Aidarhan3Geyu Liu4Haji Akber Aisa5State Key Laboratory Basis of Xinjiang Indigenous Medicinal Plants Resource Utilization, Xinjiang Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Urumqi 830011, ChinaState Key Laboratory Basis of Xinjiang Indigenous Medicinal Plants Resource Utilization, Xinjiang Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Urumqi 830011, ChinaState Key Laboratory Basis of Xinjiang Indigenous Medicinal Plants Resource Utilization, Xinjiang Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Urumqi 830011, ChinaState Key Laboratory Basis of Xinjiang Indigenous Medicinal Plants Resource Utilization, Xinjiang Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Urumqi 830011, ChinaState Key Laboratory Basis of Xinjiang Indigenous Medicinal Plants Resource Utilization, Xinjiang Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Urumqi 830011, ChinaState Key Laboratory Basis of Xinjiang Indigenous Medicinal Plants Resource Utilization, Xinjiang Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Urumqi 830011, ChinaAs one of the prominent medicinal plants listed in the Chinese pharmacopoeia (2020), <i>Saussurea involucrata</i> (Kar. et Kir.) Sch.-Bip was demonstrated to possess various therapeutic effects. In our recent research, we extracted the polysaccharides from <i>S. involucrata</i> (SIP) at optimal conditions and conducted further structure elucidation on the main fraction as well as the confirmation of its possible anti-inflammatory activity. Hence, in this work, we assessed the in vitro antioxidant activity and anti-melanogenesis effects of the crude SIP in forskolin-induced B16F10 melanoma cells. The results show that SIP possessed strong antioxidant activity and was effective in concentration-dependently decreasing melanin formation and inhibiting tyrosinase activity in forskolin-induced B16F10 cells. Based on these results, the inhibitory mechanism of melanogenesis was investigated by measuring Tyrosinase (TYR), Tyrosinase related protein-1 (TRP-1), Tyrosinase related protein-2 (TRP-2), Microphthalmia-associated transcription factor (MITF), cAMP-response element binding protein (CREB), mitogen-activated protein kinases (MAPK) signaling protein members, and β-catenin degradation in forskolin-induced B16F10 cells. The anti-melanogenesis response of SIP might be attributed to the regulation of c-Jun <i>N</i>-terminal kinase (JNK) phosphorylation and β-catenin degradation pathways. These results suggest that polysaccharides from <i>S. involucrata</i> possess a strong anti-melanogenic effect, and thus could be used as a high-value natural material for skin whitening in cosmeceutical industries.https://www.mdpi.com/2072-6643/14/23/5044anti-melanogenesisB16F10 melanoma cell<i>Saussurea involucrata</i>β-catenin degradation |
| spellingShingle | Mayila Kamilijiang Deng Zang Nuermaimaiti Abudukelimu Nurbolat Aidarhan Geyu Liu Haji Akber Aisa Anti-Melanogenesis Effect of Polysaccharide from <i>Saussurea involucrata</i> on Forskolin-Induced Melanogenesis in B16F10 Melanoma Cells Nutrients anti-melanogenesis B16F10 melanoma cell <i>Saussurea involucrata</i> β-catenin degradation |
| title | Anti-Melanogenesis Effect of Polysaccharide from <i>Saussurea involucrata</i> on Forskolin-Induced Melanogenesis in B16F10 Melanoma Cells |
| title_full | Anti-Melanogenesis Effect of Polysaccharide from <i>Saussurea involucrata</i> on Forskolin-Induced Melanogenesis in B16F10 Melanoma Cells |
| title_fullStr | Anti-Melanogenesis Effect of Polysaccharide from <i>Saussurea involucrata</i> on Forskolin-Induced Melanogenesis in B16F10 Melanoma Cells |
| title_full_unstemmed | Anti-Melanogenesis Effect of Polysaccharide from <i>Saussurea involucrata</i> on Forskolin-Induced Melanogenesis in B16F10 Melanoma Cells |
| title_short | Anti-Melanogenesis Effect of Polysaccharide from <i>Saussurea involucrata</i> on Forskolin-Induced Melanogenesis in B16F10 Melanoma Cells |
| title_sort | anti melanogenesis effect of polysaccharide from i saussurea involucrata i on forskolin induced melanogenesis in b16f10 melanoma cells |
| topic | anti-melanogenesis B16F10 melanoma cell <i>Saussurea involucrata</i> β-catenin degradation |
| url | https://www.mdpi.com/2072-6643/14/23/5044 |
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