Imaging and SERS Study of the Au Nanoparticles Interaction with HPV and Carcinogenic Cervical Tissues
In this work, gold NPs were prepared by the Turkevich method, and their interaction with HPV and cancerous cervical tissues were studied by scanning electron microscopy, energy-dispersive x-ray spectroscopy, confocal and multiphoton microscopy and SERS. The SEM images confirmed the presence and loca...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-06-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/26/12/3758 |
| _version_ | 1797529431083843584 |
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| author | Andrea Ceja-Fdez Ramon Carriles Ana Lilia González-Yebra Juan Vivero-Escoto Elder de la Rosa Tzarara López-Luke |
| author_facet | Andrea Ceja-Fdez Ramon Carriles Ana Lilia González-Yebra Juan Vivero-Escoto Elder de la Rosa Tzarara López-Luke |
| author_sort | Andrea Ceja-Fdez |
| collection | DOAJ |
| description | In this work, gold NPs were prepared by the Turkevich method, and their interaction with HPV and cancerous cervical tissues were studied by scanning electron microscopy, energy-dispersive x-ray spectroscopy, confocal and multiphoton microscopy and SERS. The SEM images confirmed the presence and localization of the gold NPs inside of the two kinds of tissues. The light absorption of the gold NPs was at 520 nm. However, it was possible to obtain two-photon imaging (red emission region) of the gold NPs inside of the tissue, exciting the samples at 900 nm, observing the morphology of the tissues. The infrared absorption was probably due to the aggregation of gold NPs inside the tissues. Therefore, through the interaction of gold nanoparticles with the HPV and cancerous cervical tissues, a surface enhanced Raman spectroscopy (SERS) was obtained. As preliminary studies, having an average of 1000 Raman spectra per tissue, SERS signals showed changes between the HPV-infected and the carcinogenic tissues; these spectral signatures occurred mainly in the DNA bands, potentially offering a tool for the rapid screening of cancer. |
| first_indexed | 2024-03-10T10:14:45Z |
| format | Article |
| id | doaj.art-7eb322aaa91345b4b58265dcb61427e4 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T10:14:45Z |
| publishDate | 2021-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-7eb322aaa91345b4b58265dcb61427e42023-11-22T00:57:33ZengMDPI AGMolecules1420-30492021-06-012612375810.3390/molecules26123758Imaging and SERS Study of the Au Nanoparticles Interaction with HPV and Carcinogenic Cervical TissuesAndrea Ceja-Fdez0Ramon Carriles1Ana Lilia González-Yebra2Juan Vivero-Escoto3Elder de la Rosa4Tzarara López-Luke5Departamento de Física Médica, División de Ciencias e Ingenierías Campus León, Universidad de Guanajuato, León 37150, MexicoCentro de Investigaciones en Óptica, A.P. 1-948, León 37150, MexicoDepartamento de Ciencias Aplicadas al Trabajo, División Ciencias de la Salud, Campus León, Universidad de Guanajuato, León 37670, MexicoDepartment of Chemistry, The University of North Carolina at Charlotte, 9201 University City Blvd., Charlotte, NC 28223, USAFacultad de Ingenierías, Campus Campestre, Universidad De La Salle Bajio, León 37150, MexicoInstituto de Investigación en Metalurgia y Materiales, Universidad Michoacana de San Nicolás de Hidalgo, Edificio U, Ciudad Universitaria, Morelia 58030, MexicoIn this work, gold NPs were prepared by the Turkevich method, and their interaction with HPV and cancerous cervical tissues were studied by scanning electron microscopy, energy-dispersive x-ray spectroscopy, confocal and multiphoton microscopy and SERS. The SEM images confirmed the presence and localization of the gold NPs inside of the two kinds of tissues. The light absorption of the gold NPs was at 520 nm. However, it was possible to obtain two-photon imaging (red emission region) of the gold NPs inside of the tissue, exciting the samples at 900 nm, observing the morphology of the tissues. The infrared absorption was probably due to the aggregation of gold NPs inside the tissues. Therefore, through the interaction of gold nanoparticles with the HPV and cancerous cervical tissues, a surface enhanced Raman spectroscopy (SERS) was obtained. As preliminary studies, having an average of 1000 Raman spectra per tissue, SERS signals showed changes between the HPV-infected and the carcinogenic tissues; these spectral signatures occurred mainly in the DNA bands, potentially offering a tool for the rapid screening of cancer.https://www.mdpi.com/1420-3049/26/12/3758gold nanoparticlesHPVcervical cancertwo-photon imagingconfocal microscopymultiphoton microscopy |
| spellingShingle | Andrea Ceja-Fdez Ramon Carriles Ana Lilia González-Yebra Juan Vivero-Escoto Elder de la Rosa Tzarara López-Luke Imaging and SERS Study of the Au Nanoparticles Interaction with HPV and Carcinogenic Cervical Tissues Molecules gold nanoparticles HPV cervical cancer two-photon imaging confocal microscopy multiphoton microscopy |
| title | Imaging and SERS Study of the Au Nanoparticles Interaction with HPV and Carcinogenic Cervical Tissues |
| title_full | Imaging and SERS Study of the Au Nanoparticles Interaction with HPV and Carcinogenic Cervical Tissues |
| title_fullStr | Imaging and SERS Study of the Au Nanoparticles Interaction with HPV and Carcinogenic Cervical Tissues |
| title_full_unstemmed | Imaging and SERS Study of the Au Nanoparticles Interaction with HPV and Carcinogenic Cervical Tissues |
| title_short | Imaging and SERS Study of the Au Nanoparticles Interaction with HPV and Carcinogenic Cervical Tissues |
| title_sort | imaging and sers study of the au nanoparticles interaction with hpv and carcinogenic cervical tissues |
| topic | gold nanoparticles HPV cervical cancer two-photon imaging confocal microscopy multiphoton microscopy |
| url | https://www.mdpi.com/1420-3049/26/12/3758 |
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