Inhibition by rno-circRNA-013017 of the apoptosis of motor neurons in anterior horn and descending axonal degeneration in rats after traumatic spinal cord injury
IntroductionSpinal cord injury (SCI) often causes continuous neurological damage to clinical patients. Circular RNAs (circRNAs) are related to a lot of diseases, including SCI. We previously found five candidate circRNAs which were likely to regulate the secondary pathophysiological changes in rat m...
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Frontiers Media S.A.
2022-12-01
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| author | Chuan Qin Chuan Qin Chuan Qin Chuan Qin Chuan Qin Chuan Qin Yi Liu Yi Liu Yi Liu Yi Liu Yi Liu Pei-Pei Xu Pei-Pei Xu Pei-Pei Xu Pei-Pei Xu Pei-Pei Xu Xin Zhang Xin Zhang Xin Zhang Xin Zhang Xin Zhang Zuliyaer Talifu Zuliyaer Talifu Zuliyaer Talifu Zuliyaer Talifu Zuliyaer Talifu Jia-Yi Liu Jia-Yi Liu Jia-Yi Liu Jia-Yi Liu Jia-Yi Liu Ying-Li Jing Ying-Li Jing Ying-Li Jing Fan Bai Fan Bai Fan Bai Li-Xi Zhao Li-Xi Zhao Li-Xi Zhao Yan Yu Yan Yu Yan Yu Feng Gao Feng Gao Feng Gao Feng Gao Feng Gao Jian-Jun Li Jian-Jun Li Jian-Jun Li Jian-Jun Li Jian-Jun Li |
| author_facet | Chuan Qin Chuan Qin Chuan Qin Chuan Qin Chuan Qin Chuan Qin Yi Liu Yi Liu Yi Liu Yi Liu Yi Liu Pei-Pei Xu Pei-Pei Xu Pei-Pei Xu Pei-Pei Xu Pei-Pei Xu Xin Zhang Xin Zhang Xin Zhang Xin Zhang Xin Zhang Zuliyaer Talifu Zuliyaer Talifu Zuliyaer Talifu Zuliyaer Talifu Zuliyaer Talifu Jia-Yi Liu Jia-Yi Liu Jia-Yi Liu Jia-Yi Liu Jia-Yi Liu Ying-Li Jing Ying-Li Jing Ying-Li Jing Fan Bai Fan Bai Fan Bai Li-Xi Zhao Li-Xi Zhao Li-Xi Zhao Yan Yu Yan Yu Yan Yu Feng Gao Feng Gao Feng Gao Feng Gao Feng Gao Jian-Jun Li Jian-Jun Li Jian-Jun Li Jian-Jun Li Jian-Jun Li |
| author_sort | Chuan Qin |
| collection | DOAJ |
| description | IntroductionSpinal cord injury (SCI) often causes continuous neurological damage to clinical patients. Circular RNAs (circRNAs) are related to a lot of diseases, including SCI. We previously found five candidate circRNAs which were likely to regulate the secondary pathophysiological changes in rat model after traumatic SCI.MethodsIn this study, we first selected and overexpressed target circRNA in rats. We then explored its functional roles using various functional assays in a rat model after SCI.ResultsWe found that rno-circRNA-013017—the selected target circRNA—reduced neuron apoptosis, preserved the survival and activity of motor neurons, and regulated apoptosis-related proteins at 3 days post-SCI using western blot, immunofluorescence and polymerase chain reaction. Additionally, we found that rno-circRNA-013017 inhibited descending axonal degeneration and preserved motor neurons and descending axons at 6 weeks post-SCI using immunofluorescence, biotin dextran amine diffusion tensor imaging. Finally, the overexpression of rno-circRNA-013017 promoted the locomotor function of rats after SCI using open-field test and gait analysis.ConclusionFocusing on the functions of rno-circRNA-013017, this study provides new options for future studies exploring therapeutic targets and molecular mechanisms for SCI. |
| first_indexed | 2024-04-11T12:50:42Z |
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| language | English |
| last_indexed | 2024-04-11T12:50:42Z |
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| spelling | doaj.art-7eb617ee19644655a9a7df69f75a70c52022-12-22T04:23:13ZengFrontiers Media S.A.Frontiers in Neuroscience1662-453X2022-12-011610.3389/fnins.2022.10658971065897Inhibition by rno-circRNA-013017 of the apoptosis of motor neurons in anterior horn and descending axonal degeneration in rats after traumatic spinal cord injuryChuan Qin0Chuan Qin1Chuan Qin2Chuan Qin3Chuan Qin4Chuan Qin5Yi Liu6Yi Liu7Yi Liu8Yi Liu9Yi Liu10Pei-Pei Xu11Pei-Pei Xu12Pei-Pei Xu13Pei-Pei Xu14Pei-Pei Xu15Xin Zhang16Xin Zhang17Xin Zhang18Xin Zhang19Xin Zhang20Zuliyaer Talifu21Zuliyaer Talifu22Zuliyaer Talifu23Zuliyaer Talifu24Zuliyaer Talifu25Jia-Yi Liu26Jia-Yi Liu27Jia-Yi Liu28Jia-Yi Liu29Jia-Yi Liu30Ying-Li Jing31Ying-Li Jing32Ying-Li Jing33Fan Bai34Fan Bai35Fan Bai36Li-Xi Zhao37Li-Xi Zhao38Li-Xi Zhao39Yan Yu40Yan Yu41Yan Yu42Feng Gao43Feng Gao44Feng Gao45Feng Gao46Feng Gao47Jian-Jun Li48Jian-Jun Li49Jian-Jun Li50Jian-Jun Li51Jian-Jun Li52Department of Urology, Beijing Friendship Hospital, Beijing, ChinaSchool of Rehabilitation Medicine, Capital Medical University, Beijing, ChinaChina Rehabilitation Science Institute, Beijing, ChinaCenter of Neural Injury and Repair, Beijing Institute for Brain Disorders, Beijing, ChinaDepartment of Spinal and Neural Functional Reconstruction, China Rehabilitation Research Center, Beijing, ChinaBeijing Key Laboratory of Neural Injury and Rehabilitation, Beijing, ChinaSchool of Rehabilitation Medicine, Capital Medical University, Beijing, ChinaCenter of Neural Injury and Repair, Beijing Institute for Brain Disorders, Beijing, ChinaDepartment of Spinal and Neural Functional Reconstruction, China Rehabilitation Research Center, Beijing, ChinaBeijing Key Laboratory of Neural Injury and Rehabilitation, Beijing, ChinaDepartment of Rehabilitation Medicine, The Second Hospital of Anhui Medical University, Hefei, ChinaSchool of Rehabilitation Medicine, Capital Medical University, Beijing, ChinaChina Rehabilitation Science Institute, Beijing, ChinaCenter of Neural Injury and Repair, Beijing Institute for Brain Disorders, Beijing, ChinaDepartment of Spinal and Neural Functional Reconstruction, China Rehabilitation Research Center, Beijing, ChinaBeijing Key Laboratory of Neural Injury and Rehabilitation, Beijing, ChinaSchool of Rehabilitation Medicine, Capital Medical University, Beijing, ChinaChina Rehabilitation Science Institute, Beijing, ChinaCenter of Neural Injury and Repair, Beijing Institute for Brain Disorders, Beijing, ChinaDepartment of Spinal and Neural Functional Reconstruction, China Rehabilitation Research Center, Beijing, ChinaBeijing Key Laboratory of Neural Injury and Rehabilitation, Beijing, ChinaSchool of Rehabilitation Medicine, Capital Medical University, Beijing, ChinaChina Rehabilitation Science Institute, Beijing, ChinaCenter of Neural Injury and Repair, Beijing Institute for Brain Disorders, Beijing, ChinaDepartment of Spinal and Neural Functional Reconstruction, China Rehabilitation Research Center, Beijing, ChinaBeijing Key Laboratory of Neural Injury and Rehabilitation, Beijing, ChinaSchool of Rehabilitation Medicine, Capital Medical University, Beijing, ChinaChina Rehabilitation Science Institute, Beijing, ChinaCenter of Neural Injury and Repair, Beijing Institute for Brain Disorders, Beijing, ChinaDepartment of Spinal and Neural Functional Reconstruction, China Rehabilitation Research Center, Beijing, ChinaBeijing Key Laboratory of Neural Injury and Rehabilitation, Beijing, ChinaChina Rehabilitation Science Institute, Beijing, ChinaCenter of Neural Injury and Repair, Beijing Institute for Brain Disorders, Beijing, ChinaBeijing Key Laboratory of Neural Injury and Rehabilitation, Beijing, ChinaChina Rehabilitation Science Institute, Beijing, ChinaCenter of Neural Injury and Repair, Beijing Institute for Brain Disorders, Beijing, ChinaBeijing Key Laboratory of Neural Injury and Rehabilitation, Beijing, ChinaChina Rehabilitation Science Institute, Beijing, ChinaCenter of Neural Injury and Repair, Beijing Institute for Brain Disorders, Beijing, ChinaBeijing Key Laboratory of Neural Injury and Rehabilitation, Beijing, ChinaChina Rehabilitation Science Institute, Beijing, ChinaCenter of Neural Injury and Repair, Beijing Institute for Brain Disorders, Beijing, ChinaBeijing Key Laboratory of Neural Injury and Rehabilitation, Beijing, ChinaSchool of Rehabilitation Medicine, Capital Medical University, Beijing, ChinaChina Rehabilitation Science Institute, Beijing, ChinaCenter of Neural Injury and Repair, Beijing Institute for Brain Disorders, Beijing, ChinaDepartment of Spinal and Neural Functional Reconstruction, China Rehabilitation Research Center, Beijing, ChinaBeijing Key Laboratory of Neural Injury and Rehabilitation, Beijing, ChinaSchool of Rehabilitation Medicine, Capital Medical University, Beijing, ChinaChina Rehabilitation Science Institute, Beijing, ChinaCenter of Neural Injury and Repair, Beijing Institute for Brain Disorders, Beijing, ChinaDepartment of Spinal and Neural Functional Reconstruction, China Rehabilitation Research Center, Beijing, ChinaBeijing Key Laboratory of Neural Injury and Rehabilitation, Beijing, ChinaIntroductionSpinal cord injury (SCI) often causes continuous neurological damage to clinical patients. Circular RNAs (circRNAs) are related to a lot of diseases, including SCI. We previously found five candidate circRNAs which were likely to regulate the secondary pathophysiological changes in rat model after traumatic SCI.MethodsIn this study, we first selected and overexpressed target circRNA in rats. We then explored its functional roles using various functional assays in a rat model after SCI.ResultsWe found that rno-circRNA-013017—the selected target circRNA—reduced neuron apoptosis, preserved the survival and activity of motor neurons, and regulated apoptosis-related proteins at 3 days post-SCI using western blot, immunofluorescence and polymerase chain reaction. Additionally, we found that rno-circRNA-013017 inhibited descending axonal degeneration and preserved motor neurons and descending axons at 6 weeks post-SCI using immunofluorescence, biotin dextran amine diffusion tensor imaging. Finally, the overexpression of rno-circRNA-013017 promoted the locomotor function of rats after SCI using open-field test and gait analysis.ConclusionFocusing on the functions of rno-circRNA-013017, this study provides new options for future studies exploring therapeutic targets and molecular mechanisms for SCI.https://www.frontiersin.org/articles/10.3389/fnins.2022.1065897/fullcircRNAsapoptosisaxonal degenerationspinal cord injuryrats |
| spellingShingle | Chuan Qin Chuan Qin Chuan Qin Chuan Qin Chuan Qin Chuan Qin Yi Liu Yi Liu Yi Liu Yi Liu Yi Liu Pei-Pei Xu Pei-Pei Xu Pei-Pei Xu Pei-Pei Xu Pei-Pei Xu Xin Zhang Xin Zhang Xin Zhang Xin Zhang Xin Zhang Zuliyaer Talifu Zuliyaer Talifu Zuliyaer Talifu Zuliyaer Talifu Zuliyaer Talifu Jia-Yi Liu Jia-Yi Liu Jia-Yi Liu Jia-Yi Liu Jia-Yi Liu Ying-Li Jing Ying-Li Jing Ying-Li Jing Fan Bai Fan Bai Fan Bai Li-Xi Zhao Li-Xi Zhao Li-Xi Zhao Yan Yu Yan Yu Yan Yu Feng Gao Feng Gao Feng Gao Feng Gao Feng Gao Jian-Jun Li Jian-Jun Li Jian-Jun Li Jian-Jun Li Jian-Jun Li Inhibition by rno-circRNA-013017 of the apoptosis of motor neurons in anterior horn and descending axonal degeneration in rats after traumatic spinal cord injury Frontiers in Neuroscience circRNAs apoptosis axonal degeneration spinal cord injury rats |
| title | Inhibition by rno-circRNA-013017 of the apoptosis of motor neurons in anterior horn and descending axonal degeneration in rats after traumatic spinal cord injury |
| title_full | Inhibition by rno-circRNA-013017 of the apoptosis of motor neurons in anterior horn and descending axonal degeneration in rats after traumatic spinal cord injury |
| title_fullStr | Inhibition by rno-circRNA-013017 of the apoptosis of motor neurons in anterior horn and descending axonal degeneration in rats after traumatic spinal cord injury |
| title_full_unstemmed | Inhibition by rno-circRNA-013017 of the apoptosis of motor neurons in anterior horn and descending axonal degeneration in rats after traumatic spinal cord injury |
| title_short | Inhibition by rno-circRNA-013017 of the apoptosis of motor neurons in anterior horn and descending axonal degeneration in rats after traumatic spinal cord injury |
| title_sort | inhibition by rno circrna 013017 of the apoptosis of motor neurons in anterior horn and descending axonal degeneration in rats after traumatic spinal cord injury |
| topic | circRNAs apoptosis axonal degeneration spinal cord injury rats |
| url | https://www.frontiersin.org/articles/10.3389/fnins.2022.1065897/full |
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