Impact of Protoporphyrin Lysine Derivatives on the Ability of <i>Nosema ceranae</i> Spores to Infect Honeybees
The effect of two protoporphyrin IX derivatives conjugated with single (PP[Lys(TFA)-OH)]<sub>2</sub>) or double (PP[Lys(TFA)-Lys(TFA)-OH]<sub>2</sub>) lysine moieties on the infectious capacity of <i>Nosema ceranae</i> spores was examined, and their efficacies wer...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-08-01
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| Series: | Insects |
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| Online Access: | https://www.mdpi.com/2075-4450/11/8/504 |
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| author | Katarzyna Buczek Kamil Deryło Mateusz Kutyła Katarzyna Rybicka-Jasińska Dorota Gryko Grzegorz Borsuk Beata Rodzik Mariusz Trytek |
| author_facet | Katarzyna Buczek Kamil Deryło Mateusz Kutyła Katarzyna Rybicka-Jasińska Dorota Gryko Grzegorz Borsuk Beata Rodzik Mariusz Trytek |
| author_sort | Katarzyna Buczek |
| collection | DOAJ |
| description | The effect of two protoporphyrin IX derivatives conjugated with single (PP[Lys(TFA)-OH)]<sub>2</sub>) or double (PP[Lys(TFA)-Lys(TFA)-OH]<sub>2</sub>) lysine moieties on the infectious capacity of <i>Nosema ceranae</i> spores was examined, and their efficacies were compared with those of a cationic porphyrin (H<sub>2</sub>TTMePP). Honeybees were inoculated with spores preincubated with porphyrins or with untreated spores (control). A significantly lower level of infection was observed in the bees infected with the porphyrin-treated spores than in the infected control. Porphyrins 1 and 2 reduced the infectious capability of microsporidia more efficiently than porphyrin 3, with bee mortality declining to almost 50%. Confocal analysis of the midguts of infected bees revealed distinct differences in the number of spores between the control group and the group infected with PP[Lys(TFA)-Lys(TFA)-OH]<sub>2</sub>-treated spores. Notably, bees with a reduced level of infection consumed less sucrose syrup than the control bees, indicating a reduction in digestive disorders and an improvement in food absorption. |
| first_indexed | 2024-03-10T17:58:03Z |
| format | Article |
| id | doaj.art-7eb9d2fd67b74dd8afaf7d71e34b2b6a |
| institution | Directory Open Access Journal |
| issn | 2075-4450 |
| language | English |
| last_indexed | 2024-03-10T17:58:03Z |
| publishDate | 2020-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Insects |
| spelling | doaj.art-7eb9d2fd67b74dd8afaf7d71e34b2b6a2023-11-20T09:05:47ZengMDPI AGInsects2075-44502020-08-0111850410.3390/insects11080504Impact of Protoporphyrin Lysine Derivatives on the Ability of <i>Nosema ceranae</i> Spores to Infect HoneybeesKatarzyna Buczek0Kamil Deryło1Mateusz Kutyła2Katarzyna Rybicka-Jasińska3Dorota Gryko4Grzegorz Borsuk5Beata Rodzik6Mariusz Trytek7Department of Industrial and Environmental Microbiology, Faculty of Biology and Biotechnology, Maria Curie-Skłodowska University, Akademicka 19, 20-033 Lublin, PolandDepartment of Molecular Biology, Faculty of Biology and Biotechnology, Maria Curie-Skłodowska University, Akademicka 19, 20-033 Lublin, PolandDepartment of Industrial and Environmental Microbiology, Faculty of Biology and Biotechnology, Maria Curie-Skłodowska University, Akademicka 19, 20-033 Lublin, PolandInstitute of Organic Chemistry, Polish Academy of Sciences, Kasprzaka 44/52, 01-224 Warsaw, PolandInstitute of Organic Chemistry, Polish Academy of Sciences, Kasprzaka 44/52, 01-224 Warsaw, PolandInstitute of Biological Basis of Animal Production, Faculty of Biology, Animal Sciences and Bioeconomy, University of Life Sciences in Lublin, Akademicka 13, 20-950 Lublin, PolandDepartment of Applied Mathematics, Faculty of Mathematics, Maria Curie-Skłodowska University, Plac Marii Curie-Skłodowskiej 1, 20-031 Lublin, PolandDepartment of Industrial and Environmental Microbiology, Faculty of Biology and Biotechnology, Maria Curie-Skłodowska University, Akademicka 19, 20-033 Lublin, PolandThe effect of two protoporphyrin IX derivatives conjugated with single (PP[Lys(TFA)-OH)]<sub>2</sub>) or double (PP[Lys(TFA)-Lys(TFA)-OH]<sub>2</sub>) lysine moieties on the infectious capacity of <i>Nosema ceranae</i> spores was examined, and their efficacies were compared with those of a cationic porphyrin (H<sub>2</sub>TTMePP). Honeybees were inoculated with spores preincubated with porphyrins or with untreated spores (control). A significantly lower level of infection was observed in the bees infected with the porphyrin-treated spores than in the infected control. Porphyrins 1 and 2 reduced the infectious capability of microsporidia more efficiently than porphyrin 3, with bee mortality declining to almost 50%. Confocal analysis of the midguts of infected bees revealed distinct differences in the number of spores between the control group and the group infected with PP[Lys(TFA)-Lys(TFA)-OH]<sub>2</sub>-treated spores. Notably, bees with a reduced level of infection consumed less sucrose syrup than the control bees, indicating a reduction in digestive disorders and an improvement in food absorption.https://www.mdpi.com/2075-4450/11/8/504infected honeybeesmicrosporidia<i>Nosema ceranae</i>feed intakeprotoporphyrin IX amides |
| spellingShingle | Katarzyna Buczek Kamil Deryło Mateusz Kutyła Katarzyna Rybicka-Jasińska Dorota Gryko Grzegorz Borsuk Beata Rodzik Mariusz Trytek Impact of Protoporphyrin Lysine Derivatives on the Ability of <i>Nosema ceranae</i> Spores to Infect Honeybees Insects infected honeybees microsporidia <i>Nosema ceranae</i> feed intake protoporphyrin IX amides |
| title | Impact of Protoporphyrin Lysine Derivatives on the Ability of <i>Nosema ceranae</i> Spores to Infect Honeybees |
| title_full | Impact of Protoporphyrin Lysine Derivatives on the Ability of <i>Nosema ceranae</i> Spores to Infect Honeybees |
| title_fullStr | Impact of Protoporphyrin Lysine Derivatives on the Ability of <i>Nosema ceranae</i> Spores to Infect Honeybees |
| title_full_unstemmed | Impact of Protoporphyrin Lysine Derivatives on the Ability of <i>Nosema ceranae</i> Spores to Infect Honeybees |
| title_short | Impact of Protoporphyrin Lysine Derivatives on the Ability of <i>Nosema ceranae</i> Spores to Infect Honeybees |
| title_sort | impact of protoporphyrin lysine derivatives on the ability of i nosema ceranae i spores to infect honeybees |
| topic | infected honeybees microsporidia <i>Nosema ceranae</i> feed intake protoporphyrin IX amides |
| url | https://www.mdpi.com/2075-4450/11/8/504 |
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