Identification of regulatory elements that control PPARγ expression in adipocyte progenitors.
Adipose tissue renewal and obesity-driven expansion of fat cell number are dependent on proliferation and differentiation of adipose progenitors that reside in the vasculature that develops in coordination with adipose depots. The transcriptional events that regulate commitment of progenitors to the...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2013-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3757023?pdf=render |
| _version_ | 1818588560096231424 |
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| author | Wen-Ling Chou Andrea Galmozzi David Partida Kevin Kwan Hui Yeung Andrew I Su Enrique Saez |
| author_facet | Wen-Ling Chou Andrea Galmozzi David Partida Kevin Kwan Hui Yeung Andrew I Su Enrique Saez |
| author_sort | Wen-Ling Chou |
| collection | DOAJ |
| description | Adipose tissue renewal and obesity-driven expansion of fat cell number are dependent on proliferation and differentiation of adipose progenitors that reside in the vasculature that develops in coordination with adipose depots. The transcriptional events that regulate commitment of progenitors to the adipose lineage are poorly understood. Because expression of the nuclear receptor PPARγ defines the adipose lineage, isolation of elements that control PPARγ expression in adipose precursors may lead to discovery of transcriptional regulators of early adipocyte determination. Here, we describe the identification and validation in transgenic mice of 5 highly conserved non-coding sequences from the PPARγ locus that can drive expression of a reporter gene in a manner that recapitulates the tissue-specific pattern of PPARγ expression. Surprisingly, these 5 elements appear to control PPARγ expression in adipocyte precursors that are associated with the vasculature of adipose depots, but not in mature adipocytes. Characterization of these five PPARγ regulatory sequences may enable isolation of the transcription factors that bind these cis elements and provide insight into the molecular regulation of adipose tissue expansion in normal and pathological states. |
| first_indexed | 2024-12-16T09:26:41Z |
| format | Article |
| id | doaj.art-7eba34dbb29a47ef9a2c46c976acbc30 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-16T09:26:41Z |
| publishDate | 2013-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-7eba34dbb29a47ef9a2c46c976acbc302022-12-21T22:36:38ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-0188e7251110.1371/journal.pone.0072511Identification of regulatory elements that control PPARγ expression in adipocyte progenitors.Wen-Ling ChouAndrea GalmozziDavid PartidaKevin KwanHui YeungAndrew I SuEnrique SaezAdipose tissue renewal and obesity-driven expansion of fat cell number are dependent on proliferation and differentiation of adipose progenitors that reside in the vasculature that develops in coordination with adipose depots. The transcriptional events that regulate commitment of progenitors to the adipose lineage are poorly understood. Because expression of the nuclear receptor PPARγ defines the adipose lineage, isolation of elements that control PPARγ expression in adipose precursors may lead to discovery of transcriptional regulators of early adipocyte determination. Here, we describe the identification and validation in transgenic mice of 5 highly conserved non-coding sequences from the PPARγ locus that can drive expression of a reporter gene in a manner that recapitulates the tissue-specific pattern of PPARγ expression. Surprisingly, these 5 elements appear to control PPARγ expression in adipocyte precursors that are associated with the vasculature of adipose depots, but not in mature adipocytes. Characterization of these five PPARγ regulatory sequences may enable isolation of the transcription factors that bind these cis elements and provide insight into the molecular regulation of adipose tissue expansion in normal and pathological states.http://europepmc.org/articles/PMC3757023?pdf=render |
| spellingShingle | Wen-Ling Chou Andrea Galmozzi David Partida Kevin Kwan Hui Yeung Andrew I Su Enrique Saez Identification of regulatory elements that control PPARγ expression in adipocyte progenitors. PLoS ONE |
| title | Identification of regulatory elements that control PPARγ expression in adipocyte progenitors. |
| title_full | Identification of regulatory elements that control PPARγ expression in adipocyte progenitors. |
| title_fullStr | Identification of regulatory elements that control PPARγ expression in adipocyte progenitors. |
| title_full_unstemmed | Identification of regulatory elements that control PPARγ expression in adipocyte progenitors. |
| title_short | Identification of regulatory elements that control PPARγ expression in adipocyte progenitors. |
| title_sort | identification of regulatory elements that control pparγ expression in adipocyte progenitors |
| url | http://europepmc.org/articles/PMC3757023?pdf=render |
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