Genome-Wide Association Study for Grain Micronutrient Concentrations in Wheat Advanced Lines Derived From Wild Emmer
Wheat is one of the important staple crops as the resources of both food and micronutrient for most people of the world. However, the levels of micronutrients (especially Fe and Zn) in common wheat are inherently low. Biofortification is an effective way to increase the micronutrient concentration o...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2021-05-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2021.651283/full |
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| author | Jia Liu Jia Liu Lin Huang Tingxuan Li Yaxi Liu Yaxi Liu Yaxi Liu Zehong Yan Zehong Yan Zehong Yan Guan Tang Youliang Zheng Youliang Zheng Youliang Zheng Dengcai Liu Dengcai Liu Dengcai Liu Bihua Wu Bihua Wu Bihua Wu |
| author_facet | Jia Liu Jia Liu Lin Huang Tingxuan Li Yaxi Liu Yaxi Liu Yaxi Liu Zehong Yan Zehong Yan Zehong Yan Guan Tang Youliang Zheng Youliang Zheng Youliang Zheng Dengcai Liu Dengcai Liu Dengcai Liu Bihua Wu Bihua Wu Bihua Wu |
| author_sort | Jia Liu |
| collection | DOAJ |
| description | Wheat is one of the important staple crops as the resources of both food and micronutrient for most people of the world. However, the levels of micronutrients (especially Fe and Zn) in common wheat are inherently low. Biofortification is an effective way to increase the micronutrient concentration of wheat. Wild emmer wheat (Triticum turgidum ssp. dicoccoides, AABB, 2n = 4x = 28) is an important germplasm resource for wheat micronutrients improvement. In the present study, a genome-wide association study (GWAS) was performed to characterize grain iron, zinc, and manganese concentration (GFeC, GZnC, and GMnC) in 161 advanced lines derived from wild emmer. Using both the general linear model and mixed linear model, we identified 14 high-confidence significant marker-trait associations (MTAs) that were associated with GFeC, GZnC, and GMnC of which nine MTAs were novel. Six MTAs distributed on chromosomes 3B, 4A, 4B, 5A, and 7B were significantly associated with GFeC. Three MTAs on 1A and 2A were significantly associated with GZnC and five MTAs on 1B were significantly associated with GMnC. These MTAs show no negative effects on thousand kernel weight (TKW), implying the potential value for simultaneous improvement of micronutrient concentrations and TKW in breeding. Meanwhile, the GFeC, GZnC and GMnC are positively correlated, suggesting that these traits could be simultaneously improved. Genotypes containing high-confidence MTAs and 61 top genotypes with a higher concentration of grain micronutrients were recommended for wheat biofortification breeding. A total of 38 candidate genes related to micronutrient concentrations were identified. These candidates can be classified into four main groups: enzymes, transporter proteins, MYB transcription factor, and plant defense responses proteins. The MTAs and associated candidate genes provide essential information for wheat biofortification breeding through marker-assisted selection (MAS). |
| first_indexed | 2024-12-16T23:09:19Z |
| format | Article |
| id | doaj.art-7ebaee7ef9504376814b123a4393fc9f |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-16T23:09:19Z |
| publishDate | 2021-05-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Plant Science |
| spelling | doaj.art-7ebaee7ef9504376814b123a4393fc9f2022-12-21T22:12:28ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2021-05-011210.3389/fpls.2021.651283651283Genome-Wide Association Study for Grain Micronutrient Concentrations in Wheat Advanced Lines Derived From Wild EmmerJia Liu0Jia Liu1Lin Huang2Tingxuan Li3Yaxi Liu4Yaxi Liu5Yaxi Liu6Zehong Yan7Zehong Yan8Zehong Yan9Guan Tang10Youliang Zheng11Youliang Zheng12Youliang Zheng13Dengcai Liu14Dengcai Liu15Dengcai Liu16Bihua Wu17Bihua Wu18Bihua Wu19State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Wenjiang, ChinaTriticeae Research Institute, Sichuan Agricultural University, Wenjiang, ChinaTriticeae Research Institute, Sichuan Agricultural University, Wenjiang, ChinaCollege of Resources, Sichuan Agricultural University, Wenjiang, ChinaState Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Wenjiang, ChinaTriticeae Research Institute, Sichuan Agricultural University, Wenjiang, ChinaKey Laboratory of Crop Genetic Resources and Improvement, Ministry of Education, Sichuan Agricultural University, Ya'an, ChinaState Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Wenjiang, ChinaTriticeae Research Institute, Sichuan Agricultural University, Wenjiang, ChinaKey Laboratory of Crop Genetic Resources and Improvement, Ministry of Education, Sichuan Agricultural University, Ya'an, ChinaTriticeae Research Institute, Sichuan Agricultural University, Wenjiang, ChinaState Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Wenjiang, ChinaTriticeae Research Institute, Sichuan Agricultural University, Wenjiang, ChinaKey Laboratory of Crop Genetic Resources and Improvement, Ministry of Education, Sichuan Agricultural University, Ya'an, ChinaState Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Wenjiang, ChinaTriticeae Research Institute, Sichuan Agricultural University, Wenjiang, ChinaKey Laboratory of Crop Genetic Resources and Improvement, Ministry of Education, Sichuan Agricultural University, Ya'an, ChinaState Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Wenjiang, ChinaTriticeae Research Institute, Sichuan Agricultural University, Wenjiang, ChinaKey Laboratory of Crop Genetic Resources and Improvement, Ministry of Education, Sichuan Agricultural University, Ya'an, ChinaWheat is one of the important staple crops as the resources of both food and micronutrient for most people of the world. However, the levels of micronutrients (especially Fe and Zn) in common wheat are inherently low. Biofortification is an effective way to increase the micronutrient concentration of wheat. Wild emmer wheat (Triticum turgidum ssp. dicoccoides, AABB, 2n = 4x = 28) is an important germplasm resource for wheat micronutrients improvement. In the present study, a genome-wide association study (GWAS) was performed to characterize grain iron, zinc, and manganese concentration (GFeC, GZnC, and GMnC) in 161 advanced lines derived from wild emmer. Using both the general linear model and mixed linear model, we identified 14 high-confidence significant marker-trait associations (MTAs) that were associated with GFeC, GZnC, and GMnC of which nine MTAs were novel. Six MTAs distributed on chromosomes 3B, 4A, 4B, 5A, and 7B were significantly associated with GFeC. Three MTAs on 1A and 2A were significantly associated with GZnC and five MTAs on 1B were significantly associated with GMnC. These MTAs show no negative effects on thousand kernel weight (TKW), implying the potential value for simultaneous improvement of micronutrient concentrations and TKW in breeding. Meanwhile, the GFeC, GZnC and GMnC are positively correlated, suggesting that these traits could be simultaneously improved. Genotypes containing high-confidence MTAs and 61 top genotypes with a higher concentration of grain micronutrients were recommended for wheat biofortification breeding. A total of 38 candidate genes related to micronutrient concentrations were identified. These candidates can be classified into four main groups: enzymes, transporter proteins, MYB transcription factor, and plant defense responses proteins. The MTAs and associated candidate genes provide essential information for wheat biofortification breeding through marker-assisted selection (MAS).https://www.frontiersin.org/articles/10.3389/fpls.2021.651283/fullwild emmercommon wheatgrain micronutrient concentrationswide hybridizationGWASbiofortification |
| spellingShingle | Jia Liu Jia Liu Lin Huang Tingxuan Li Yaxi Liu Yaxi Liu Yaxi Liu Zehong Yan Zehong Yan Zehong Yan Guan Tang Youliang Zheng Youliang Zheng Youliang Zheng Dengcai Liu Dengcai Liu Dengcai Liu Bihua Wu Bihua Wu Bihua Wu Genome-Wide Association Study for Grain Micronutrient Concentrations in Wheat Advanced Lines Derived From Wild Emmer Frontiers in Plant Science wild emmer common wheat grain micronutrient concentrations wide hybridization GWAS biofortification |
| title | Genome-Wide Association Study for Grain Micronutrient Concentrations in Wheat Advanced Lines Derived From Wild Emmer |
| title_full | Genome-Wide Association Study for Grain Micronutrient Concentrations in Wheat Advanced Lines Derived From Wild Emmer |
| title_fullStr | Genome-Wide Association Study for Grain Micronutrient Concentrations in Wheat Advanced Lines Derived From Wild Emmer |
| title_full_unstemmed | Genome-Wide Association Study for Grain Micronutrient Concentrations in Wheat Advanced Lines Derived From Wild Emmer |
| title_short | Genome-Wide Association Study for Grain Micronutrient Concentrations in Wheat Advanced Lines Derived From Wild Emmer |
| title_sort | genome wide association study for grain micronutrient concentrations in wheat advanced lines derived from wild emmer |
| topic | wild emmer common wheat grain micronutrient concentrations wide hybridization GWAS biofortification |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2021.651283/full |
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