Thiolated Mesoporous Silica Nanoparticles as an Immunoadjuvant to Enhance Efficacy of Intravesical Chemotherapy for Bladder Cancer
Abstract The characteristics of global prevalence and high recurrence of bladder cancer has led numerous efforts to develop new treatments. The spontaneous voiding and degradation of the chemodrug hamper the efficacy and effectiveness of intravesical chemotherapy following tumor resection. Herein, t...
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| Format: | Article |
| Language: | English |
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Wiley
2023-03-01
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| Series: | Advanced Science |
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| Online Access: | https://doi.org/10.1002/advs.202204643 |
| _version_ | 1827999340162973696 |
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| author | Cheng‐Che Chen Yu‐Chen Fa Yen‐Yu Kuo Yi‐Chun Liu Chih‐Yu Lin Xin‐Hui Wang Yu‐Huan Lu Yu‐Han Chiang Chia‐Min Yang Li‐Chen Wu Ja‐an Annie Ho |
| author_facet | Cheng‐Che Chen Yu‐Chen Fa Yen‐Yu Kuo Yi‐Chun Liu Chih‐Yu Lin Xin‐Hui Wang Yu‐Huan Lu Yu‐Han Chiang Chia‐Min Yang Li‐Chen Wu Ja‐an Annie Ho |
| author_sort | Cheng‐Che Chen |
| collection | DOAJ |
| description | Abstract The characteristics of global prevalence and high recurrence of bladder cancer has led numerous efforts to develop new treatments. The spontaneous voiding and degradation of the chemodrug hamper the efficacy and effectiveness of intravesical chemotherapy following tumor resection. Herein, the externally thiolated hollow mesoporous silica nanoparticles (MSN‐SH(E)) is fabricated to serve as a platform for improved bladder intravesical therapy. Enhanced mucoadhesive effect of the thiolated nanovector is confirmed with porcine bladder. The permeation‐enhancing effect is also verified, and a fragmented distribution pattern of a tight junction protein, claudin‐4, indicates the opening of tight junction. Moreover, MSN‐SH(E)‐associated reprogramming of M2 macrophages to M1‐like phenotype is observed in vitro. The antitumor activity of the mitomycin C (MMC)‐loaded nanovector (MMC@MSN‐SH(E)) is more effective than that of MMC alone in both in vitro and in vivo. In addition, IHC staining is used to analyze IFN‐γ, TGF‐β1, and TNF‐α. These observations substantiated the significance of MMC@MSN‐SH(E) in promoting anticancer activity, holding the great potential for being used in intravesical therapy for non‐muscle invasive bladder cancer (NMIBC) due to its mucoadhesivity, enhanced permeation, immunomodulation, and prolonged and very efficient drug exposure. |
| first_indexed | 2024-04-10T05:57:23Z |
| format | Article |
| id | doaj.art-7ebdc28946794ac78b9603c8ddf122ec |
| institution | Directory Open Access Journal |
| issn | 2198-3844 |
| language | English |
| last_indexed | 2024-04-10T05:57:23Z |
| publishDate | 2023-03-01 |
| publisher | Wiley |
| record_format | Article |
| series | Advanced Science |
| spelling | doaj.art-7ebdc28946794ac78b9603c8ddf122ec2023-03-03T08:59:14ZengWileyAdvanced Science2198-38442023-03-01107n/an/a10.1002/advs.202204643Thiolated Mesoporous Silica Nanoparticles as an Immunoadjuvant to Enhance Efficacy of Intravesical Chemotherapy for Bladder CancerCheng‐Che Chen0Yu‐Chen Fa1Yen‐Yu Kuo2Yi‐Chun Liu3Chih‐Yu Lin4Xin‐Hui Wang5Yu‐Huan Lu6Yu‐Han Chiang7Chia‐Min Yang8Li‐Chen Wu9Ja‐an Annie Ho10BioAnalytical Chemistry and Nanobiomedicine Laboratory Department of Biochemical Science and Technology National Taiwan University 10617 Taipei TaiwanBioAnalytical Chemistry and Nanobiomedicine Laboratory Department of Biochemical Science and Technology National Taiwan University 10617 Taipei TaiwanDepartment of Chemistry National Tsing Hua University 300044 Hsinchu TaiwanBioAnalytical Chemistry and Nanobiomedicine Laboratory Department of Biochemical Science and Technology National Taiwan University 10617 Taipei TaiwanDepartment of Chemistry National Tsing Hua University 300044 Hsinchu TaiwanInstrumentation Center National Taiwan University 10617 Taipei TaiwanDepartment of Chemistry National Tsing Hua University 300044 Hsinchu TaiwanDepartment of Chemistry National Taiwan University 10617 Taipei TaiwanDepartment of Chemistry National Tsing Hua University 300044 Hsinchu TaiwanDepartment of Applied Chemistry National Chi Nan University Puli Nantou 54561 TaiwanBioAnalytical Chemistry and Nanobiomedicine Laboratory Department of Biochemical Science and Technology National Taiwan University 10617 Taipei TaiwanAbstract The characteristics of global prevalence and high recurrence of bladder cancer has led numerous efforts to develop new treatments. The spontaneous voiding and degradation of the chemodrug hamper the efficacy and effectiveness of intravesical chemotherapy following tumor resection. Herein, the externally thiolated hollow mesoporous silica nanoparticles (MSN‐SH(E)) is fabricated to serve as a platform for improved bladder intravesical therapy. Enhanced mucoadhesive effect of the thiolated nanovector is confirmed with porcine bladder. The permeation‐enhancing effect is also verified, and a fragmented distribution pattern of a tight junction protein, claudin‐4, indicates the opening of tight junction. Moreover, MSN‐SH(E)‐associated reprogramming of M2 macrophages to M1‐like phenotype is observed in vitro. The antitumor activity of the mitomycin C (MMC)‐loaded nanovector (MMC@MSN‐SH(E)) is more effective than that of MMC alone in both in vitro and in vivo. In addition, IHC staining is used to analyze IFN‐γ, TGF‐β1, and TNF‐α. These observations substantiated the significance of MMC@MSN‐SH(E) in promoting anticancer activity, holding the great potential for being used in intravesical therapy for non‐muscle invasive bladder cancer (NMIBC) due to its mucoadhesivity, enhanced permeation, immunomodulation, and prolonged and very efficient drug exposure.https://doi.org/10.1002/advs.202204643bladder cancerintravesical therapymesoporous silica nanoparticlemitomycin Cthiol modification |
| spellingShingle | Cheng‐Che Chen Yu‐Chen Fa Yen‐Yu Kuo Yi‐Chun Liu Chih‐Yu Lin Xin‐Hui Wang Yu‐Huan Lu Yu‐Han Chiang Chia‐Min Yang Li‐Chen Wu Ja‐an Annie Ho Thiolated Mesoporous Silica Nanoparticles as an Immunoadjuvant to Enhance Efficacy of Intravesical Chemotherapy for Bladder Cancer Advanced Science bladder cancer intravesical therapy mesoporous silica nanoparticle mitomycin C thiol modification |
| title | Thiolated Mesoporous Silica Nanoparticles as an Immunoadjuvant to Enhance Efficacy of Intravesical Chemotherapy for Bladder Cancer |
| title_full | Thiolated Mesoporous Silica Nanoparticles as an Immunoadjuvant to Enhance Efficacy of Intravesical Chemotherapy for Bladder Cancer |
| title_fullStr | Thiolated Mesoporous Silica Nanoparticles as an Immunoadjuvant to Enhance Efficacy of Intravesical Chemotherapy for Bladder Cancer |
| title_full_unstemmed | Thiolated Mesoporous Silica Nanoparticles as an Immunoadjuvant to Enhance Efficacy of Intravesical Chemotherapy for Bladder Cancer |
| title_short | Thiolated Mesoporous Silica Nanoparticles as an Immunoadjuvant to Enhance Efficacy of Intravesical Chemotherapy for Bladder Cancer |
| title_sort | thiolated mesoporous silica nanoparticles as an immunoadjuvant to enhance efficacy of intravesical chemotherapy for bladder cancer |
| topic | bladder cancer intravesical therapy mesoporous silica nanoparticle mitomycin C thiol modification |
| url | https://doi.org/10.1002/advs.202204643 |
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