Agave Fructans in Oaxaca’s Emblematic Specimens: <i>Agave angustifolia</i> Haw. and <i>Agave potatorum</i> Zucc.
Despite the recognition of <i>Agave tequilana</i> Weber var. Azul as raw material for producing tequila and obtaining prebiotics, there are other highly relevant <i>Agave</i> species in Mexico. Oaxaca contains a startlingly diverse range of <i>Agave</i> species; &...
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MDPI AG
2022-07-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/11/14/1834 |
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| author | Ruth E. Márquez-López Patricia Araceli Santiago-García Mercedes G. López |
| author_facet | Ruth E. Márquez-López Patricia Araceli Santiago-García Mercedes G. López |
| author_sort | Ruth E. Márquez-López |
| collection | DOAJ |
| description | Despite the recognition of <i>Agave tequilana</i> Weber var. Azul as raw material for producing tequila and obtaining prebiotics, there are other highly relevant <i>Agave</i> species in Mexico. Oaxaca contains a startlingly diverse range of <i>Agave</i> species; <i>Agave angustifolia</i> Haw. and <i>Agave potatorum</i> Zucc. are two classic specimens with great commercial potential. In this study, we examined the fructan fluctuation in these two species during their lifetime in the field (from 1 to 6 years old). First, we analyzed their morphological diversity based on vegetative characteristics. Subsequently, fructan extracts were analyzed by TLC, FT-IR, and HPAEC-PAD to identify carbohydrates. Multivariate analyses of the morphological parameters indicated a morphological divergence between the two species. Furthermore, we found that the concentration of simple carbohydrates and fructans, as well as the fructan DP, changed during plant development. Glucose, fructose, and fructooligosaccharides (FOS) were more abundant in <i>A. potatorum</i>, while <i>A. angustifolia</i> showed a greater amount of sucrose and fructans with a high DP. Fructan DP heatmaps were constructed using HPAEC-PAD profiles—the heatmaps were very helpful for establishing an easy correlation between age and the carbohydrate types present in the fructan extracts. This study is an important contribution to the agave fructan knowledge of the Mexican agave diversity. |
| first_indexed | 2024-03-09T10:13:31Z |
| format | Article |
| id | doaj.art-7ebeaa83c5b042ac888728989f0e37e3 |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-09T10:13:31Z |
| publishDate | 2022-07-01 |
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| record_format | Article |
| series | Plants |
| spelling | doaj.art-7ebeaa83c5b042ac888728989f0e37e32023-12-01T22:35:45ZengMDPI AGPlants2223-77472022-07-011114183410.3390/plants11141834Agave Fructans in Oaxaca’s Emblematic Specimens: <i>Agave angustifolia</i> Haw. and <i>Agave potatorum</i> Zucc.Ruth E. Márquez-López0Patricia Araceli Santiago-García1Mercedes G. López2Instituto Politécnico Nacional, Centro Interdisciplinario de Investigación Para el Desarrollo Integral Regional—Unidad Oaxaca, Oaxaca 71230, MexicoInstituto Politécnico Nacional, Centro Interdisciplinario de Investigación Para el Desarrollo Integral Regional—Unidad Oaxaca, Oaxaca 71230, MexicoDepartamento de Biotecnología y Bioquímica, Centro de Investigación y de Estudios Avanzados del IPN—Unidad Irapuato, Guanajuato 36824, MexicoDespite the recognition of <i>Agave tequilana</i> Weber var. Azul as raw material for producing tequila and obtaining prebiotics, there are other highly relevant <i>Agave</i> species in Mexico. Oaxaca contains a startlingly diverse range of <i>Agave</i> species; <i>Agave angustifolia</i> Haw. and <i>Agave potatorum</i> Zucc. are two classic specimens with great commercial potential. In this study, we examined the fructan fluctuation in these two species during their lifetime in the field (from 1 to 6 years old). First, we analyzed their morphological diversity based on vegetative characteristics. Subsequently, fructan extracts were analyzed by TLC, FT-IR, and HPAEC-PAD to identify carbohydrates. Multivariate analyses of the morphological parameters indicated a morphological divergence between the two species. Furthermore, we found that the concentration of simple carbohydrates and fructans, as well as the fructan DP, changed during plant development. Glucose, fructose, and fructooligosaccharides (FOS) were more abundant in <i>A. potatorum</i>, while <i>A. angustifolia</i> showed a greater amount of sucrose and fructans with a high DP. Fructan DP heatmaps were constructed using HPAEC-PAD profiles—the heatmaps were very helpful for establishing an easy correlation between age and the carbohydrate types present in the fructan extracts. This study is an important contribution to the agave fructan knowledge of the Mexican agave diversity.https://www.mdpi.com/2223-7747/11/14/1834agavefructansagedegree of polymerizationFT-IRHPAEC-PAD |
| spellingShingle | Ruth E. Márquez-López Patricia Araceli Santiago-García Mercedes G. López Agave Fructans in Oaxaca’s Emblematic Specimens: <i>Agave angustifolia</i> Haw. and <i>Agave potatorum</i> Zucc. Plants agave fructans age degree of polymerization FT-IR HPAEC-PAD |
| title | Agave Fructans in Oaxaca’s Emblematic Specimens: <i>Agave angustifolia</i> Haw. and <i>Agave potatorum</i> Zucc. |
| title_full | Agave Fructans in Oaxaca’s Emblematic Specimens: <i>Agave angustifolia</i> Haw. and <i>Agave potatorum</i> Zucc. |
| title_fullStr | Agave Fructans in Oaxaca’s Emblematic Specimens: <i>Agave angustifolia</i> Haw. and <i>Agave potatorum</i> Zucc. |
| title_full_unstemmed | Agave Fructans in Oaxaca’s Emblematic Specimens: <i>Agave angustifolia</i> Haw. and <i>Agave potatorum</i> Zucc. |
| title_short | Agave Fructans in Oaxaca’s Emblematic Specimens: <i>Agave angustifolia</i> Haw. and <i>Agave potatorum</i> Zucc. |
| title_sort | agave fructans in oaxaca s emblematic specimens i agave angustifolia i haw and i agave potatorum i zucc |
| topic | agave fructans age degree of polymerization FT-IR HPAEC-PAD |
| url | https://www.mdpi.com/2223-7747/11/14/1834 |
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