Comparative Study of the Antioxidant and Anti-Inflammatory Effects of Leaf Extracts from Four Different <i>Morus alba</i> Genotypes in High Fat Diet-Induced Obesity in Mice
Increased levels of reactive oxygen species (ROS) and a low-grade chronic inflammation in multiple organs have been demonstrated in obesity. <i>Morus alba</i> leaves extracts (MAEs) have been used in traditional medicine as anti-inflammatory agents. In this work, the bioactive compounds...
| Main Authors: | , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2020-08-01
|
| Series: | Antioxidants |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-3921/9/8/733 |
| _version_ | 1797558881040203776 |
|---|---|
| author | Francisco Javier Leyva-Jiménez Antonio Jesús Ruiz-Malagón José Alberto Molina-Tijeras Patricia Diez-Echave Teresa Vezza Laura Hidalgo-García Jesús Lozano-Sánchez David Arráez-Román José Luis Cenis Antonio Abel Lozano-Pérez Alba Rodríguez-Nogales Antonio Segura-Carretero Julio Gálvez |
| author_facet | Francisco Javier Leyva-Jiménez Antonio Jesús Ruiz-Malagón José Alberto Molina-Tijeras Patricia Diez-Echave Teresa Vezza Laura Hidalgo-García Jesús Lozano-Sánchez David Arráez-Román José Luis Cenis Antonio Abel Lozano-Pérez Alba Rodríguez-Nogales Antonio Segura-Carretero Julio Gálvez |
| author_sort | Francisco Javier Leyva-Jiménez |
| collection | DOAJ |
| description | Increased levels of reactive oxygen species (ROS) and a low-grade chronic inflammation in multiple organs have been demonstrated in obesity. <i>Morus alba</i> leaves extracts (MAEs) have been used in traditional medicine as anti-inflammatory agents. In this work, the bioactive compounds of different genotypes of <i>M. alba L.</i> (<i>Filipina</i>, <i>Valenciana Temprana</i>, <i>Kokuso</i>, and <i>Italia</i>) were analyzed not only by reverse phase high performance liquid chromatography–electrospray ionization-time of flight-mass spectrometry (RP-HPLC-ESI-TOF-MS) and hydrophilic interaction chromatography–electrospray ionization-time of flight-mass spectrometry (HILIC-ESI-TOF-MS), but also screened for in vitro and in vivo antioxidant activity by means of DPPH• radical scavenging assay and <i>Caenorhabditis elegans</i> model. These MAEs were administered daily in a model of diet-induced obesity in mice. <i>Filipina</i> and <i>Italia</i> genotypes significantly reduced weight gain, the glycemic levels in high fat diet, as well as, levels of LDL-cholesterol and triglycerides. <i>Filipina</i> and <i>Italia</i> MAEs also reduced the expression of proinflammatory mediators such as <i>Tnf-α</i>, <i>Il-1β</i>, <i>Il-6</i> and increased the levels of adiponectin and AMPK, which exert anti-inflammatory effects. Moreover, <i>Italia</i> genotype ameliorated the intestinal barrier function. In conclusion, <i>Filipina</i> and <i>Italia</i> methanolic extracts show the highest antioxidant and anti-inflammatory effect, due to the presence of compounds such as protocatechuic acid or quercetin-3-glucoside, and they could be developed as a complementary treatment for obesity and metabolic disorders. |
| first_indexed | 2024-03-10T17:37:37Z |
| format | Article |
| id | doaj.art-7ec38715228246fab75114a04c4236ad |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-10T17:37:37Z |
| publishDate | 2020-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-7ec38715228246fab75114a04c4236ad2023-11-20T09:48:42ZengMDPI AGAntioxidants2076-39212020-08-019873310.3390/antiox9080733Comparative Study of the Antioxidant and Anti-Inflammatory Effects of Leaf Extracts from Four Different <i>Morus alba</i> Genotypes in High Fat Diet-Induced Obesity in MiceFrancisco Javier Leyva-Jiménez0Antonio Jesús Ruiz-Malagón1José Alberto Molina-Tijeras2Patricia Diez-Echave3Teresa Vezza4Laura Hidalgo-García5Jesús Lozano-Sánchez6David Arráez-Román7José Luis Cenis8Antonio Abel Lozano-Pérez9Alba Rodríguez-Nogales10Antonio Segura-Carretero11Julio Gálvez12Research and Development Functional Food Centre, Health Science Technological Park, Avenida del Conocimiento 37, E-18016 Granada, SpainCIBER-EHD, Department of Pharmacology, Center for Biomedical Research (CIBM), University of Granada, 18071 Granada, SpainCIBER-EHD, Department of Pharmacology, Center for Biomedical Research (CIBM), University of Granada, 18071 Granada, SpainCIBER-EHD, Department of Pharmacology, Center for Biomedical Research (CIBM), University of Granada, 18071 Granada, SpainCIBER-EHD, Department of Pharmacology, Center for Biomedical Research (CIBM), University of Granada, 18071 Granada, SpainCIBER-EHD, Department of Pharmacology, Center for Biomedical Research (CIBM), University of Granada, 18071 Granada, SpainResearch and Development Functional Food Centre, Health Science Technological Park, Avenida del Conocimiento 37, E-18016 Granada, SpainResearch and Development Functional Food Centre, Health Science Technological Park, Avenida del Conocimiento 37, E-18016 Granada, SpainDepartamento de Biotecnología, Genómica y Mejora Vegetal, Instituto Murciano de Investigación y Desarrollo Agrario y Alimentario, 30150 La Alberca (Murcia), SpainDepartamento de Biotecnología, Genómica y Mejora Vegetal, Instituto Murciano de Investigación y Desarrollo Agrario y Alimentario, 30150 La Alberca (Murcia), SpainCIBER-EHD, Department of Pharmacology, Center for Biomedical Research (CIBM), University of Granada, 18071 Granada, SpainResearch and Development Functional Food Centre, Health Science Technological Park, Avenida del Conocimiento 37, E-18016 Granada, SpainCIBER-EHD, Department of Pharmacology, Center for Biomedical Research (CIBM), University of Granada, 18071 Granada, SpainIncreased levels of reactive oxygen species (ROS) and a low-grade chronic inflammation in multiple organs have been demonstrated in obesity. <i>Morus alba</i> leaves extracts (MAEs) have been used in traditional medicine as anti-inflammatory agents. In this work, the bioactive compounds of different genotypes of <i>M. alba L.</i> (<i>Filipina</i>, <i>Valenciana Temprana</i>, <i>Kokuso</i>, and <i>Italia</i>) were analyzed not only by reverse phase high performance liquid chromatography–electrospray ionization-time of flight-mass spectrometry (RP-HPLC-ESI-TOF-MS) and hydrophilic interaction chromatography–electrospray ionization-time of flight-mass spectrometry (HILIC-ESI-TOF-MS), but also screened for in vitro and in vivo antioxidant activity by means of DPPH• radical scavenging assay and <i>Caenorhabditis elegans</i> model. These MAEs were administered daily in a model of diet-induced obesity in mice. <i>Filipina</i> and <i>Italia</i> genotypes significantly reduced weight gain, the glycemic levels in high fat diet, as well as, levels of LDL-cholesterol and triglycerides. <i>Filipina</i> and <i>Italia</i> MAEs also reduced the expression of proinflammatory mediators such as <i>Tnf-α</i>, <i>Il-1β</i>, <i>Il-6</i> and increased the levels of adiponectin and AMPK, which exert anti-inflammatory effects. Moreover, <i>Italia</i> genotype ameliorated the intestinal barrier function. In conclusion, <i>Filipina</i> and <i>Italia</i> methanolic extracts show the highest antioxidant and anti-inflammatory effect, due to the presence of compounds such as protocatechuic acid or quercetin-3-glucoside, and they could be developed as a complementary treatment for obesity and metabolic disorders.https://www.mdpi.com/2076-3921/9/8/733<i>Morus alba</i> leaveshigh fat dietobesitychemical characterizationanti-inflammatorymetabolic disorders |
| spellingShingle | Francisco Javier Leyva-Jiménez Antonio Jesús Ruiz-Malagón José Alberto Molina-Tijeras Patricia Diez-Echave Teresa Vezza Laura Hidalgo-García Jesús Lozano-Sánchez David Arráez-Román José Luis Cenis Antonio Abel Lozano-Pérez Alba Rodríguez-Nogales Antonio Segura-Carretero Julio Gálvez Comparative Study of the Antioxidant and Anti-Inflammatory Effects of Leaf Extracts from Four Different <i>Morus alba</i> Genotypes in High Fat Diet-Induced Obesity in Mice Antioxidants <i>Morus alba</i> leaves high fat diet obesity chemical characterization anti-inflammatory metabolic disorders |
| title | Comparative Study of the Antioxidant and Anti-Inflammatory Effects of Leaf Extracts from Four Different <i>Morus alba</i> Genotypes in High Fat Diet-Induced Obesity in Mice |
| title_full | Comparative Study of the Antioxidant and Anti-Inflammatory Effects of Leaf Extracts from Four Different <i>Morus alba</i> Genotypes in High Fat Diet-Induced Obesity in Mice |
| title_fullStr | Comparative Study of the Antioxidant and Anti-Inflammatory Effects of Leaf Extracts from Four Different <i>Morus alba</i> Genotypes in High Fat Diet-Induced Obesity in Mice |
| title_full_unstemmed | Comparative Study of the Antioxidant and Anti-Inflammatory Effects of Leaf Extracts from Four Different <i>Morus alba</i> Genotypes in High Fat Diet-Induced Obesity in Mice |
| title_short | Comparative Study of the Antioxidant and Anti-Inflammatory Effects of Leaf Extracts from Four Different <i>Morus alba</i> Genotypes in High Fat Diet-Induced Obesity in Mice |
| title_sort | comparative study of the antioxidant and anti inflammatory effects of leaf extracts from four different i morus alba i genotypes in high fat diet induced obesity in mice |
| topic | <i>Morus alba</i> leaves high fat diet obesity chemical characterization anti-inflammatory metabolic disorders |
| url | https://www.mdpi.com/2076-3921/9/8/733 |
| work_keys_str_mv | AT franciscojavierleyvajimenez comparativestudyoftheantioxidantandantiinflammatoryeffectsofleafextractsfromfourdifferentimorusalbaigenotypesinhighfatdietinducedobesityinmice AT antoniojesusruizmalagon comparativestudyoftheantioxidantandantiinflammatoryeffectsofleafextractsfromfourdifferentimorusalbaigenotypesinhighfatdietinducedobesityinmice AT josealbertomolinatijeras comparativestudyoftheantioxidantandantiinflammatoryeffectsofleafextractsfromfourdifferentimorusalbaigenotypesinhighfatdietinducedobesityinmice AT patriciadiezechave comparativestudyoftheantioxidantandantiinflammatoryeffectsofleafextractsfromfourdifferentimorusalbaigenotypesinhighfatdietinducedobesityinmice AT teresavezza comparativestudyoftheantioxidantandantiinflammatoryeffectsofleafextractsfromfourdifferentimorusalbaigenotypesinhighfatdietinducedobesityinmice AT laurahidalgogarcia comparativestudyoftheantioxidantandantiinflammatoryeffectsofleafextractsfromfourdifferentimorusalbaigenotypesinhighfatdietinducedobesityinmice AT jesuslozanosanchez comparativestudyoftheantioxidantandantiinflammatoryeffectsofleafextractsfromfourdifferentimorusalbaigenotypesinhighfatdietinducedobesityinmice AT davidarraezroman comparativestudyoftheantioxidantandantiinflammatoryeffectsofleafextractsfromfourdifferentimorusalbaigenotypesinhighfatdietinducedobesityinmice AT joseluiscenis comparativestudyoftheantioxidantandantiinflammatoryeffectsofleafextractsfromfourdifferentimorusalbaigenotypesinhighfatdietinducedobesityinmice AT antonioabellozanoperez comparativestudyoftheantioxidantandantiinflammatoryeffectsofleafextractsfromfourdifferentimorusalbaigenotypesinhighfatdietinducedobesityinmice AT albarodrigueznogales comparativestudyoftheantioxidantandantiinflammatoryeffectsofleafextractsfromfourdifferentimorusalbaigenotypesinhighfatdietinducedobesityinmice AT antonioseguracarretero comparativestudyoftheantioxidantandantiinflammatoryeffectsofleafextractsfromfourdifferentimorusalbaigenotypesinhighfatdietinducedobesityinmice AT juliogalvez comparativestudyoftheantioxidantandantiinflammatoryeffectsofleafextractsfromfourdifferentimorusalbaigenotypesinhighfatdietinducedobesityinmice |