Analysis of Environmental DNA and Edaphic Factors for the Detection of the Snail Intermediate Host Oncomelania hupensis quadrasi
Background: The perpetuation of schistosomiasis japonica in the Philippines depends to a major extent on the persistence of its intermediate host Oncomelania hupensis quadrasi, an amphibious snail. While the malacological survey remains the method of choice in determining the contamination of the en...
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| Format: | Article |
| Language: | English |
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MDPI AG
2019-09-01
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| Series: | Pathogens |
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| Online Access: | https://www.mdpi.com/2076-0817/8/4/160 |
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| author | Fritz Ivy C. Calata Camille Z. Caranguian Jillian Ela M. Mendoza Raffy Jay C. Fornillos Ian Kim B. Tabios Ian Kendrich C. Fontanilla Lydia R. Leonardo Louie S. Sunico Satoru Kawai Yuichi Chigusa Mihoko Kikuchi Megumi Sato Toshifumi Minamoto Zenaida G. Baoanan Marcello Otake Sato |
| author_facet | Fritz Ivy C. Calata Camille Z. Caranguian Jillian Ela M. Mendoza Raffy Jay C. Fornillos Ian Kim B. Tabios Ian Kendrich C. Fontanilla Lydia R. Leonardo Louie S. Sunico Satoru Kawai Yuichi Chigusa Mihoko Kikuchi Megumi Sato Toshifumi Minamoto Zenaida G. Baoanan Marcello Otake Sato |
| author_sort | Fritz Ivy C. Calata |
| collection | DOAJ |
| description | Background: The perpetuation of schistosomiasis japonica in the Philippines depends to a major extent on the persistence of its intermediate host Oncomelania hupensis quadrasi, an amphibious snail. While the malacological survey remains the method of choice in determining the contamination of the environment as evidenced by snails infected with schistosome larval stages, an emerging technology known as environmental DNA (eDNA) detection provides an alternative method. Previous reports showed that O. hupensis quadrasi eDNA could be detected in water, but no reports have been made on its detection in soil. Methods: This study, thus focused on the detection of O. hupensis quadrasi eDNA from soil samples collected from two selected schistosomiasis-endemic barangays in Gonzaga, Cagayan Valley using conventional and TaqMan-quantitative (qPCR) PCRs. Results: The results show that qPCR could better detect O. hupensis quadrasi eDNA in soil than the conventional method. In determining the possible distribution range of the snail, basic edaphic factors were measured and correlated with the presence of eDNA. The eDNA detection probability increases as the pH, phosphorous, zinc, copper, and potassium content increases, possibly indicating the conditions in the environment that favor the presence of the snails. A map was generated to show the probable extent of the distribution of the snails away from the body of the freshwater. Conclusion: The information generated from this study could be used to determine snail habitats that could be possible hotspots of transmission and should, therefore, be targeted for snail control or be fenced off from human and animal contact or from the contamination of feces by being a dumping site for domestic wastes. |
| first_indexed | 2024-04-11T20:40:44Z |
| format | Article |
| id | doaj.art-7ec4c09c2a8c4e4492235c4de8429621 |
| institution | Directory Open Access Journal |
| issn | 2076-0817 |
| language | English |
| last_indexed | 2024-04-11T20:40:44Z |
| publishDate | 2019-09-01 |
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| series | Pathogens |
| spelling | doaj.art-7ec4c09c2a8c4e4492235c4de84296212022-12-22T04:04:13ZengMDPI AGPathogens2076-08172019-09-018416010.3390/pathogens8040160pathogens8040160Analysis of Environmental DNA and Edaphic Factors for the Detection of the Snail Intermediate Host Oncomelania hupensis quadrasiFritz Ivy C. Calata0Camille Z. Caranguian1Jillian Ela M. Mendoza2Raffy Jay C. Fornillos3Ian Kim B. Tabios4Ian Kendrich C. Fontanilla5Lydia R. Leonardo6Louie S. Sunico7Satoru Kawai8Yuichi Chigusa9Mihoko Kikuchi10Megumi Sato11Toshifumi Minamoto12Zenaida G. Baoanan13Marcello Otake Sato14Department of Biology, College of Science, University of the Philippines Baguio, Governor Pack Road, Baguio City 2600, PhilippinesDepartment of Biology, College of Science, University of the Philippines Baguio, Governor Pack Road, Baguio City 2600, PhilippinesDepartment of Biology, College of Science, University of the Philippines Baguio, Governor Pack Road, Baguio City 2600, PhilippinesDNA Barcoding Laboratory, College of Science, National Science Complex, University of the Philippines Diliman, Quezon City 1101, PhilippinesCollege of Medicine, University of the Philippines Manila, Pedro Gil St. Ermita, Manila 1000, PhilippinesDNA Barcoding Laboratory, College of Science, National Science Complex, University of the Philippines Diliman, Quezon City 1101, PhilippinesDNA Barcoding Laboratory, College of Science, National Science Complex, University of the Philippines Diliman, Quezon City 1101, PhilippinesRural Health Unit, Municipal Health Office, Gonzaga, Cagayan Valley 3515, PhilippinesDepartment of Tropical Medicine and Parasitology, Dokkyo Medical University, 880 Kitakobayashi, Mibu-machi, Shimotsuga-gun, Tochigi 321-0293, JapanDepartment of Tropical Medicine and Parasitology, Dokkyo Medical University, 880 Kitakobayashi, Mibu-machi, Shimotsuga-gun, Tochigi 321-0293, JapanDepartment of Immunogenetics, Institute of Tropical Medicine, Nagasaki University, 1-12-4 Sakamoto, Nagasaki 852-8523, JapanGraduate School of Health Sciences, Niigata University 2-746 Asahimachi-dori, Chuo-ku, Niigata 951-8518, JapanGraduate School of Human Development and Environment, Kobe University, 3-11, Tsurukabuto, Nada-ku, Kobe 657-8501, JapanDepartment of Biology, College of Science, University of the Philippines Baguio, Governor Pack Road, Baguio City 2600, PhilippinesDepartment of Tropical Medicine and Parasitology, Dokkyo Medical University, 880 Kitakobayashi, Mibu-machi, Shimotsuga-gun, Tochigi 321-0293, JapanBackground: The perpetuation of schistosomiasis japonica in the Philippines depends to a major extent on the persistence of its intermediate host Oncomelania hupensis quadrasi, an amphibious snail. While the malacological survey remains the method of choice in determining the contamination of the environment as evidenced by snails infected with schistosome larval stages, an emerging technology known as environmental DNA (eDNA) detection provides an alternative method. Previous reports showed that O. hupensis quadrasi eDNA could be detected in water, but no reports have been made on its detection in soil. Methods: This study, thus focused on the detection of O. hupensis quadrasi eDNA from soil samples collected from two selected schistosomiasis-endemic barangays in Gonzaga, Cagayan Valley using conventional and TaqMan-quantitative (qPCR) PCRs. Results: The results show that qPCR could better detect O. hupensis quadrasi eDNA in soil than the conventional method. In determining the possible distribution range of the snail, basic edaphic factors were measured and correlated with the presence of eDNA. The eDNA detection probability increases as the pH, phosphorous, zinc, copper, and potassium content increases, possibly indicating the conditions in the environment that favor the presence of the snails. A map was generated to show the probable extent of the distribution of the snails away from the body of the freshwater. Conclusion: The information generated from this study could be used to determine snail habitats that could be possible hotspots of transmission and should, therefore, be targeted for snail control or be fenced off from human and animal contact or from the contamination of feces by being a dumping site for domestic wastes.https://www.mdpi.com/2076-0817/8/4/160Oncomelania hupensis quadrasischistosomiasis japonicaenvironmental DNAedaphic factorssnail surveillance |
| spellingShingle | Fritz Ivy C. Calata Camille Z. Caranguian Jillian Ela M. Mendoza Raffy Jay C. Fornillos Ian Kim B. Tabios Ian Kendrich C. Fontanilla Lydia R. Leonardo Louie S. Sunico Satoru Kawai Yuichi Chigusa Mihoko Kikuchi Megumi Sato Toshifumi Minamoto Zenaida G. Baoanan Marcello Otake Sato Analysis of Environmental DNA and Edaphic Factors for the Detection of the Snail Intermediate Host Oncomelania hupensis quadrasi Pathogens Oncomelania hupensis quadrasi schistosomiasis japonica environmental DNA edaphic factors snail surveillance |
| title | Analysis of Environmental DNA and Edaphic Factors for the Detection of the Snail Intermediate Host Oncomelania hupensis quadrasi |
| title_full | Analysis of Environmental DNA and Edaphic Factors for the Detection of the Snail Intermediate Host Oncomelania hupensis quadrasi |
| title_fullStr | Analysis of Environmental DNA and Edaphic Factors for the Detection of the Snail Intermediate Host Oncomelania hupensis quadrasi |
| title_full_unstemmed | Analysis of Environmental DNA and Edaphic Factors for the Detection of the Snail Intermediate Host Oncomelania hupensis quadrasi |
| title_short | Analysis of Environmental DNA and Edaphic Factors for the Detection of the Snail Intermediate Host Oncomelania hupensis quadrasi |
| title_sort | analysis of environmental dna and edaphic factors for the detection of the snail intermediate host oncomelania hupensis quadrasi |
| topic | Oncomelania hupensis quadrasi schistosomiasis japonica environmental DNA edaphic factors snail surveillance |
| url | https://www.mdpi.com/2076-0817/8/4/160 |
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