Perilipin Isoforms and PGC-1α Are Regulated Differentially in Rat Heart during Pregnancy-Induced Physiological Cardiac Hypertrophy
<i>Background and Objectives</i>: Perilipins 1–5 (PLIN) are lipid droplet-associated proteins that participate in regulating lipid storage and metabolism, and the PLIN5 isoform is known to form a nuclear complex with peroxisome proliferator-activated receptor-gamma coactivator 1-alpha (P...
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MDPI AG
2022-10-01
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| Online Access: | https://www.mdpi.com/1648-9144/58/10/1433 |
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| author | Jesús A. Rosas-Rodríguez Adolfo Virgen-Ortíz Enrico A. Ruiz Rudy M. Ortiz José G. Soñanez-Organis |
| author_facet | Jesús A. Rosas-Rodríguez Adolfo Virgen-Ortíz Enrico A. Ruiz Rudy M. Ortiz José G. Soñanez-Organis |
| author_sort | Jesús A. Rosas-Rodríguez |
| collection | DOAJ |
| description | <i>Background and Objectives</i>: Perilipins 1–5 (PLIN) are lipid droplet-associated proteins that participate in regulating lipid storage and metabolism, and the PLIN5 isoform is known to form a nuclear complex with peroxisome proliferator-activated receptor-gamma coactivator 1-alpha (PGC-1α) to regulate lipid metabolism gene expression. However, the changes in PLIN isoforms’ expression in response to pregnancy-induced cardiac hypertrophy are not thoroughly studied. The aim of this study was to quantify the mRNA expression of PLIN isoforms and PGC-1α along with total triacylglycerol (TAG) and cholesterol levels during late pregnancy and the postpartum period in the rat left ventricle. <i>Materials and Methods</i>: Female Sprague-Dawley rats were divided into three groups: non-pregnant, late pregnancy, and postpartum. The mRNA and protein levels were evaluated using quantitative RT-PCR and Western blotting, respectively. TAG and total cholesterol content were evaluated using commercial colorimetric methods. <i>Results</i>: The expression of mRNAs for PLIN1, 2, and 5 increased during pregnancy and the postpartum period. PGC-1α mRNA and protein expression increased during pregnancy and the postpartum period. Moreover, TAG and total cholesterol increased during pregnancy and returned to basal levels after pregnancy. <i>Conclusions</i>: Our results demonstrate that pregnancy upregulates differentially the expression of PLIN isoforms along with PGC-1α, suggesting that together they might be involved in the regulation of the lipid metabolic shift induced by pregnancy. |
| first_indexed | 2024-03-09T19:51:17Z |
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| language | English |
| last_indexed | 2024-03-09T19:51:17Z |
| publishDate | 2022-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Medicina |
| spelling | doaj.art-7ec4d074ce12435bba8f68494496517e2023-11-24T01:11:06ZengMDPI AGMedicina1010-660X1648-91442022-10-015810143310.3390/medicina58101433Perilipin Isoforms and PGC-1α Are Regulated Differentially in Rat Heart during Pregnancy-Induced Physiological Cardiac HypertrophyJesús A. Rosas-Rodríguez0Adolfo Virgen-Ortíz1Enrico A. Ruiz2Rudy M. Ortiz3José G. Soñanez-Organis4Departamento de Ciencias Químico-Biológicas y Agropecuarias, Universidad de Sonora, Lázaro Cárdenas del Río No. 100, Francisco Villa, Navojoa CP 85880, MexicoCentro Universitario de Investigaciones Biomédicas, Universidad de Colima, Colima 28040, MexicoDepartamento de Zoología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Ciudad de México 07738, MexicoDepartment of Molecular & Cell Biology, University of California Merced, Merced, CA 95343, USADepartamento de Ciencias Químico-Biológicas y Agropecuarias, Universidad de Sonora, Lázaro Cárdenas del Río No. 100, Francisco Villa, Navojoa CP 85880, Mexico<i>Background and Objectives</i>: Perilipins 1–5 (PLIN) are lipid droplet-associated proteins that participate in regulating lipid storage and metabolism, and the PLIN5 isoform is known to form a nuclear complex with peroxisome proliferator-activated receptor-gamma coactivator 1-alpha (PGC-1α) to regulate lipid metabolism gene expression. However, the changes in PLIN isoforms’ expression in response to pregnancy-induced cardiac hypertrophy are not thoroughly studied. The aim of this study was to quantify the mRNA expression of PLIN isoforms and PGC-1α along with total triacylglycerol (TAG) and cholesterol levels during late pregnancy and the postpartum period in the rat left ventricle. <i>Materials and Methods</i>: Female Sprague-Dawley rats were divided into three groups: non-pregnant, late pregnancy, and postpartum. The mRNA and protein levels were evaluated using quantitative RT-PCR and Western blotting, respectively. TAG and total cholesterol content were evaluated using commercial colorimetric methods. <i>Results</i>: The expression of mRNAs for PLIN1, 2, and 5 increased during pregnancy and the postpartum period. PGC-1α mRNA and protein expression increased during pregnancy and the postpartum period. Moreover, TAG and total cholesterol increased during pregnancy and returned to basal levels after pregnancy. <i>Conclusions</i>: Our results demonstrate that pregnancy upregulates differentially the expression of PLIN isoforms along with PGC-1α, suggesting that together they might be involved in the regulation of the lipid metabolic shift induced by pregnancy.https://www.mdpi.com/1648-9144/58/10/1433perilipinlipid metabolismpregnancygene expressionprotein abundancePGC-1α |
| spellingShingle | Jesús A. Rosas-Rodríguez Adolfo Virgen-Ortíz Enrico A. Ruiz Rudy M. Ortiz José G. Soñanez-Organis Perilipin Isoforms and PGC-1α Are Regulated Differentially in Rat Heart during Pregnancy-Induced Physiological Cardiac Hypertrophy Medicina perilipin lipid metabolism pregnancy gene expression protein abundance PGC-1α |
| title | Perilipin Isoforms and PGC-1α Are Regulated Differentially in Rat Heart during Pregnancy-Induced Physiological Cardiac Hypertrophy |
| title_full | Perilipin Isoforms and PGC-1α Are Regulated Differentially in Rat Heart during Pregnancy-Induced Physiological Cardiac Hypertrophy |
| title_fullStr | Perilipin Isoforms and PGC-1α Are Regulated Differentially in Rat Heart during Pregnancy-Induced Physiological Cardiac Hypertrophy |
| title_full_unstemmed | Perilipin Isoforms and PGC-1α Are Regulated Differentially in Rat Heart during Pregnancy-Induced Physiological Cardiac Hypertrophy |
| title_short | Perilipin Isoforms and PGC-1α Are Regulated Differentially in Rat Heart during Pregnancy-Induced Physiological Cardiac Hypertrophy |
| title_sort | perilipin isoforms and pgc 1α are regulated differentially in rat heart during pregnancy induced physiological cardiac hypertrophy |
| topic | perilipin lipid metabolism pregnancy gene expression protein abundance PGC-1α |
| url | https://www.mdpi.com/1648-9144/58/10/1433 |
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