Immunization of Chickens with the Enterobactin Conjugate Vaccine Reduced <i>Campylobacter jejuni</i> Colonization in the Intestine
<i>Campylobacter jejuni</i> is the leading bacterial cause of human enteritis in developed countries. Chicken is the major animal reservoir of <i>C. jejuni</i> and a powerful infection model for human campylobacteriosis. No commercial vaccine against <i>C. jejuni</i&...
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| Format: | Article |
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MDPI AG
2020-12-01
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| Series: | Vaccines |
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| Online Access: | https://www.mdpi.com/2076-393X/8/4/747 |
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| author | Yifang Cui Fangfang Guo Jie Guo Xiaoya Cao Huiwen Wang Bing Yang Hongzhuan Zhou Xia Su Ximin Zeng Jun Lin Fuzhou Xu |
| author_facet | Yifang Cui Fangfang Guo Jie Guo Xiaoya Cao Huiwen Wang Bing Yang Hongzhuan Zhou Xia Su Ximin Zeng Jun Lin Fuzhou Xu |
| author_sort | Yifang Cui |
| collection | DOAJ |
| description | <i>Campylobacter jejuni</i> is the leading bacterial cause of human enteritis in developed countries. Chicken is the major animal reservoir of <i>C. jejuni</i> and a powerful infection model for human campylobacteriosis. No commercial vaccine against <i>C. jejuni</i> is available to date. The high affinity iron acquisition mediated through enterobactin (Ent), a small siderophore, plays a critical role in the colonization of <i>C. jejuni</i> in the intestine. Recently, an innovative Ent conjugate vaccine has been demonstrated to induce high-level of Ent-specific antibodies in rabbits; the Ent-specific antibodies displayed potent binding ability to Ent and inhibited Ent-dependent growth of <i>C. jejuni</i>. In this study, using specific-pathogen-free (SPF) chickens, we performed three trials to evaluate the immunogenicity of the Ent conjugate vaccine and its efficacy to control <i>C. jejuni</i> colonization in the intestine. The purified Ent was conjugated to the carrier keyhole limpet hemocyanin (KLH). Intramuscular immunization of chickens with the Ent–KLH conjugate for up to three times did not affect the body weight gain, the development of major immune organs and the gut microbiota. In the first two trials, immunizations of chickens with different regimens (two or three times of vaccination) consistently induced strong Ent-specific immune response when compared to control group. Consistent with the high-level of systemic anti-Ent IgG, <i>C. jejuni</i> colonization was significantly reduced by 3–4 log<sub>10</sub> units in the cecum in two independent vaccination trials. The third trial demonstrated that single Ent–KLH vaccination is sufficient to elicit high level of systemic Ent-specific antibodies, which could persist for up to eight weeks in chickens. Taken together, the Ent–KLH conjugate vaccine could induce high-level of Ent-specific antibodies in chickens and confer host protection against <i>C. jejuni</i> colonization, which provides a novel strategy for <i>Campylobacter</i> control in poultry and humans. |
| first_indexed | 2024-03-10T14:13:45Z |
| format | Article |
| id | doaj.art-7ec83a291ddb4636aee8d4fbbb66fa23 |
| institution | Directory Open Access Journal |
| issn | 2076-393X |
| language | English |
| last_indexed | 2024-03-10T14:13:45Z |
| publishDate | 2020-12-01 |
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| record_format | Article |
| series | Vaccines |
| spelling | doaj.art-7ec83a291ddb4636aee8d4fbbb66fa232023-11-21T00:00:39ZengMDPI AGVaccines2076-393X2020-12-018474710.3390/vaccines8040747Immunization of Chickens with the Enterobactin Conjugate Vaccine Reduced <i>Campylobacter jejuni</i> Colonization in the IntestineYifang Cui0Fangfang Guo1Jie Guo2Xiaoya Cao3Huiwen Wang4Bing Yang5Hongzhuan Zhou6Xia Su7Ximin Zeng8Jun Lin9Fuzhou Xu10Beijing Key Laboratory for Prevention and Control of Infectious Diseases in Livestock and Poultry, Institute of Animal Husbandry and Veterinary Medicine, Beijing Academy of Agricultural and Forestry Sciences, Beijing 100097, ChinaBeijing Key Laboratory for Prevention and Control of Infectious Diseases in Livestock and Poultry, Institute of Animal Husbandry and Veterinary Medicine, Beijing Academy of Agricultural and Forestry Sciences, Beijing 100097, ChinaBeijing Key Laboratory for Prevention and Control of Infectious Diseases in Livestock and Poultry, Institute of Animal Husbandry and Veterinary Medicine, Beijing Academy of Agricultural and Forestry Sciences, Beijing 100097, ChinaBeijing Key Laboratory for Prevention and Control of Infectious Diseases in Livestock and Poultry, Institute of Animal Husbandry and Veterinary Medicine, Beijing Academy of Agricultural and Forestry Sciences, Beijing 100097, ChinaDepartment of Animal Science, The University of Tennessee, Knoxville, TN 37996, USABeijing Key Laboratory for Prevention and Control of Infectious Diseases in Livestock and Poultry, Institute of Animal Husbandry and Veterinary Medicine, Beijing Academy of Agricultural and Forestry Sciences, Beijing 100097, ChinaBeijing Key Laboratory for Prevention and Control of Infectious Diseases in Livestock and Poultry, Institute of Animal Husbandry and Veterinary Medicine, Beijing Academy of Agricultural and Forestry Sciences, Beijing 100097, ChinaBeijing Key Laboratory for Prevention and Control of Infectious Diseases in Livestock and Poultry, Institute of Animal Husbandry and Veterinary Medicine, Beijing Academy of Agricultural and Forestry Sciences, Beijing 100097, ChinaDepartment of Animal Science, The University of Tennessee, Knoxville, TN 37996, USADepartment of Animal Science, The University of Tennessee, Knoxville, TN 37996, USABeijing Key Laboratory for Prevention and Control of Infectious Diseases in Livestock and Poultry, Institute of Animal Husbandry and Veterinary Medicine, Beijing Academy of Agricultural and Forestry Sciences, Beijing 100097, China<i>Campylobacter jejuni</i> is the leading bacterial cause of human enteritis in developed countries. Chicken is the major animal reservoir of <i>C. jejuni</i> and a powerful infection model for human campylobacteriosis. No commercial vaccine against <i>C. jejuni</i> is available to date. The high affinity iron acquisition mediated through enterobactin (Ent), a small siderophore, plays a critical role in the colonization of <i>C. jejuni</i> in the intestine. Recently, an innovative Ent conjugate vaccine has been demonstrated to induce high-level of Ent-specific antibodies in rabbits; the Ent-specific antibodies displayed potent binding ability to Ent and inhibited Ent-dependent growth of <i>C. jejuni</i>. In this study, using specific-pathogen-free (SPF) chickens, we performed three trials to evaluate the immunogenicity of the Ent conjugate vaccine and its efficacy to control <i>C. jejuni</i> colonization in the intestine. The purified Ent was conjugated to the carrier keyhole limpet hemocyanin (KLH). Intramuscular immunization of chickens with the Ent–KLH conjugate for up to three times did not affect the body weight gain, the development of major immune organs and the gut microbiota. In the first two trials, immunizations of chickens with different regimens (two or three times of vaccination) consistently induced strong Ent-specific immune response when compared to control group. Consistent with the high-level of systemic anti-Ent IgG, <i>C. jejuni</i> colonization was significantly reduced by 3–4 log<sub>10</sub> units in the cecum in two independent vaccination trials. The third trial demonstrated that single Ent–KLH vaccination is sufficient to elicit high level of systemic Ent-specific antibodies, which could persist for up to eight weeks in chickens. Taken together, the Ent–KLH conjugate vaccine could induce high-level of Ent-specific antibodies in chickens and confer host protection against <i>C. jejuni</i> colonization, which provides a novel strategy for <i>Campylobacter</i> control in poultry and humans.https://www.mdpi.com/2076-393X/8/4/747enterobactinconjugate vaccine<i>Campylobacter jejuni</i>chickencolonization |
| spellingShingle | Yifang Cui Fangfang Guo Jie Guo Xiaoya Cao Huiwen Wang Bing Yang Hongzhuan Zhou Xia Su Ximin Zeng Jun Lin Fuzhou Xu Immunization of Chickens with the Enterobactin Conjugate Vaccine Reduced <i>Campylobacter jejuni</i> Colonization in the Intestine Vaccines enterobactin conjugate vaccine <i>Campylobacter jejuni</i> chicken colonization |
| title | Immunization of Chickens with the Enterobactin Conjugate Vaccine Reduced <i>Campylobacter jejuni</i> Colonization in the Intestine |
| title_full | Immunization of Chickens with the Enterobactin Conjugate Vaccine Reduced <i>Campylobacter jejuni</i> Colonization in the Intestine |
| title_fullStr | Immunization of Chickens with the Enterobactin Conjugate Vaccine Reduced <i>Campylobacter jejuni</i> Colonization in the Intestine |
| title_full_unstemmed | Immunization of Chickens with the Enterobactin Conjugate Vaccine Reduced <i>Campylobacter jejuni</i> Colonization in the Intestine |
| title_short | Immunization of Chickens with the Enterobactin Conjugate Vaccine Reduced <i>Campylobacter jejuni</i> Colonization in the Intestine |
| title_sort | immunization of chickens with the enterobactin conjugate vaccine reduced i campylobacter jejuni i colonization in the intestine |
| topic | enterobactin conjugate vaccine <i>Campylobacter jejuni</i> chicken colonization |
| url | https://www.mdpi.com/2076-393X/8/4/747 |
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