The identification of acetylcholine and choline in oat seedlings by gas chromatography and nuclear magnetic resonance (NMR)
Four methods of isolation and purification of choline esters from green 7-day-old oat ( Avena sativa L. cv. Diadem) seedlings were tested The results showed that the best recovery of acetylcholine and choline from plant tissues was obtained using an extraction solution composed of 15% 1N formic acid...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Polish Botanical Society
2014-01-01
|
| Series: | Acta Societatis Botanicorum Poloniae |
| Subjects: | |
| Online Access: | https://pbsociety.org.pl/journals/index.php/asbp/article/view/3018 |
| _version_ | 1811223522909880320 |
|---|---|
| author | Andrzej Tretyn Wiesława Bobkiewicz Małgorzata Tretyn Leszek Michalski |
| author_facet | Andrzej Tretyn Wiesława Bobkiewicz Małgorzata Tretyn Leszek Michalski |
| author_sort | Andrzej Tretyn |
| collection | DOAJ |
| description | Four methods of isolation and purification of choline esters from green 7-day-old oat ( Avena sativa L. cv. Diadem) seedlings were tested The results showed that the best recovery of acetylcholine and choline from plant tissues was obtained using an extraction solution composed of 15% 1N formic acid and 85%, acetone followed by precipitation of both these substances with ammonium reineckate. The presence of acetylcholine and choline in the plant extracts was confirmed by nuclear magnetic resonance (NMR) and gas chromatography. In the case of gas chromatography, after isolation and purification of the studied compounds from the plant material, estrification of choline followed by N-demethylation of acetylcholine and estrified choline were performed The demethylation reaction was conducted in a reaction mixture of 50 mM sodium thiophenolate and 25 mM thiophenol in anhydrous acetone. After its completion, the mixture was removed with pentanone and the demethylated esters were extracted into chloroform. |
| first_indexed | 2024-04-12T08:34:06Z |
| format | Article |
| id | doaj.art-7eccfc2ce8ec44a4a6982a4facf00e82 |
| institution | Directory Open Access Journal |
| issn | 2083-9480 |
| language | English |
| last_indexed | 2024-04-12T08:34:06Z |
| publishDate | 2014-01-01 |
| publisher | Polish Botanical Society |
| record_format | Article |
| series | Acta Societatis Botanicorum Poloniae |
| spelling | doaj.art-7eccfc2ce8ec44a4a6982a4facf00e822022-12-22T03:40:04ZengPolish Botanical SocietyActa Societatis Botanicorum Poloniae2083-94802014-01-0156349951110.5586/asbp.1987.0462496The identification of acetylcholine and choline in oat seedlings by gas chromatography and nuclear magnetic resonance (NMR)Andrzej Tretyn0Wiesława Bobkiewicz1Małgorzata Tretyn2Leszek Michalski3Nicolaus Copernicus UniversityNicolaus Copernicus UniversityNicolaus Copernicus UniversityNicolaus Copernicus UniversityFour methods of isolation and purification of choline esters from green 7-day-old oat ( Avena sativa L. cv. Diadem) seedlings were tested The results showed that the best recovery of acetylcholine and choline from plant tissues was obtained using an extraction solution composed of 15% 1N formic acid and 85%, acetone followed by precipitation of both these substances with ammonium reineckate. The presence of acetylcholine and choline in the plant extracts was confirmed by nuclear magnetic resonance (NMR) and gas chromatography. In the case of gas chromatography, after isolation and purification of the studied compounds from the plant material, estrification of choline followed by N-demethylation of acetylcholine and estrified choline were performed The demethylation reaction was conducted in a reaction mixture of 50 mM sodium thiophenolate and 25 mM thiophenol in anhydrous acetone. After its completion, the mixture was removed with pentanone and the demethylated esters were extracted into chloroform.https://pbsociety.org.pl/journals/index.php/asbp/article/view/3018acetylchnlinecholinegas chromatoyrnphyH-NMRArena sativa |
| spellingShingle | Andrzej Tretyn Wiesława Bobkiewicz Małgorzata Tretyn Leszek Michalski The identification of acetylcholine and choline in oat seedlings by gas chromatography and nuclear magnetic resonance (NMR) Acta Societatis Botanicorum Poloniae acetylchnline choline gas chromatoyrnphy H-NMR Arena sativa |
| title | The identification of acetylcholine and choline in oat seedlings by gas chromatography and nuclear magnetic resonance (NMR) |
| title_full | The identification of acetylcholine and choline in oat seedlings by gas chromatography and nuclear magnetic resonance (NMR) |
| title_fullStr | The identification of acetylcholine and choline in oat seedlings by gas chromatography and nuclear magnetic resonance (NMR) |
| title_full_unstemmed | The identification of acetylcholine and choline in oat seedlings by gas chromatography and nuclear magnetic resonance (NMR) |
| title_short | The identification of acetylcholine and choline in oat seedlings by gas chromatography and nuclear magnetic resonance (NMR) |
| title_sort | identification of acetylcholine and choline in oat seedlings by gas chromatography and nuclear magnetic resonance nmr |
| topic | acetylchnline choline gas chromatoyrnphy H-NMR Arena sativa |
| url | https://pbsociety.org.pl/journals/index.php/asbp/article/view/3018 |
| work_keys_str_mv | AT andrzejtretyn theidentificationofacetylcholineandcholineinoatseedlingsbygaschromatographyandnuclearmagneticresonancenmr AT wiesławabobkiewicz theidentificationofacetylcholineandcholineinoatseedlingsbygaschromatographyandnuclearmagneticresonancenmr AT małgorzatatretyn theidentificationofacetylcholineandcholineinoatseedlingsbygaschromatographyandnuclearmagneticresonancenmr AT leszekmichalski theidentificationofacetylcholineandcholineinoatseedlingsbygaschromatographyandnuclearmagneticresonancenmr AT andrzejtretyn identificationofacetylcholineandcholineinoatseedlingsbygaschromatographyandnuclearmagneticresonancenmr AT wiesławabobkiewicz identificationofacetylcholineandcholineinoatseedlingsbygaschromatographyandnuclearmagneticresonancenmr AT małgorzatatretyn identificationofacetylcholineandcholineinoatseedlingsbygaschromatographyandnuclearmagneticresonancenmr AT leszekmichalski identificationofacetylcholineandcholineinoatseedlingsbygaschromatographyandnuclearmagneticresonancenmr |