A Prospective Cohort Protocol for the Remnant Investigation in Sepsis Study
BACKGROUND:. Sepsis is a common and deadly syndrome, accounting for more than 11 million deaths annually. To mature a deeper understanding of the host and pathogen mechanisms contributing to poor outcomes in sepsis, and thereby possibly inform new therapeutic targets, sophisticated, and expensive bi...
| Main Authors: | , , , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wolters Kluwer
2023-11-01
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| Series: | Critical Care Explorations |
| Online Access: | http://journals.lww.com/10.1097/CCE.0000000000000974 |
| _version_ | 1797292762165411840 |
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| author | Christopher W. Seymour, MD, MSc Kelly Lynn Urbanek, MS Anna Nakayama Jason N. Kennedy, MS Rachel Powell, MD Renã A.S. Robinson, PhD Kathryn L. Kapp, BS Timothy R. Billiar, MD Yoram Vodovotz, PhD Stacy L. Gelhaus, PhD Vaughn S. Cooper, PhD Lu Tang, PhD Flo Mayr, MD Katherine M. Reitz, MD, MSc Christopher Horvat, MD Nuala J. Meyer, MD, MS Robert P. Dickson Derek Angus, MD Octavia Peck Palmer, PhD |
| author_facet | Christopher W. Seymour, MD, MSc Kelly Lynn Urbanek, MS Anna Nakayama Jason N. Kennedy, MS Rachel Powell, MD Renã A.S. Robinson, PhD Kathryn L. Kapp, BS Timothy R. Billiar, MD Yoram Vodovotz, PhD Stacy L. Gelhaus, PhD Vaughn S. Cooper, PhD Lu Tang, PhD Flo Mayr, MD Katherine M. Reitz, MD, MSc Christopher Horvat, MD Nuala J. Meyer, MD, MS Robert P. Dickson Derek Angus, MD Octavia Peck Palmer, PhD |
| author_sort | Christopher W. Seymour, MD, MSc |
| collection | DOAJ |
| description | BACKGROUND:. Sepsis is a common and deadly syndrome, accounting for more than 11 million deaths annually. To mature a deeper understanding of the host and pathogen mechanisms contributing to poor outcomes in sepsis, and thereby possibly inform new therapeutic targets, sophisticated, and expensive biorepositories are typically required. We propose that remnant biospecimens are an alternative for mechanistic sepsis research, although the viability and scientific value of such remnants are unknown.
METHODS AND RESULTS:. The Remnant Biospecimen Investigation in Sepsis study is a prospective cohort study of 225 adults (age ≥ 18 yr) presenting to the emergency department with community sepsis, defined as sepsis-3 criteria within 6 hours of arrival. The primary objective was to determine the scientific value of a remnant biospecimen repository in sepsis linked to clinical phenotyping in the electronic health record. We will study candidate multiomic readouts of sepsis biology, governed by a conceptual model, and determine the precision, accuracy, integrity, and comparability of proteins, small molecules, lipids, and pathogen sequencing in remnant biospecimens compared with paired biospecimens obtained according to research protocols. Paired biospecimens will include plasma from sodium–heparin, EDTA, sodium fluoride, and citrate tubes.
CONCLUSIONS:. The study has received approval from the University of Pittsburgh Human Research Protection Office (Study 21120013). Recruitment began on October 25, 2022, with planned release of primary results anticipated in 2024. Results will be made available to the public, the funders, critical care societies, laboratory medicine scientists, and other researchers. |
| first_indexed | 2024-03-07T20:02:12Z |
| format | Article |
| id | doaj.art-7ed111b6ecb34b9ea933120437ef9700 |
| institution | Directory Open Access Journal |
| issn | 2639-8028 |
| language | English |
| last_indexed | 2024-03-07T20:02:12Z |
| publishDate | 2023-11-01 |
| publisher | Wolters Kluwer |
| record_format | Article |
| series | Critical Care Explorations |
| spelling | doaj.art-7ed111b6ecb34b9ea933120437ef97002024-02-28T06:47:13ZengWolters KluwerCritical Care Explorations2639-80282023-11-01511e097410.1097/CCE.0000000000000974202311000-00005A Prospective Cohort Protocol for the Remnant Investigation in Sepsis StudyChristopher W. Seymour, MD, MSc0Kelly Lynn Urbanek, MS1Anna Nakayama2Jason N. Kennedy, MS3Rachel Powell, MD4Renã A.S. Robinson, PhD5Kathryn L. Kapp, BS6Timothy R. Billiar, MD7Yoram Vodovotz, PhD8Stacy L. Gelhaus, PhD9Vaughn S. Cooper, PhD10Lu Tang, PhD11Flo Mayr, MD12Katherine M. Reitz, MD, MSc13Christopher Horvat, MD14Nuala J. Meyer, MD, MS15Robert P. Dickson16Derek Angus, MD17Octavia Peck Palmer, PhD181 Department of Critical Care Medicine, University of Pittsburgh School of Medicine, Pittsburgh, PA1 Department of Critical Care Medicine, University of Pittsburgh School of Medicine, Pittsburgh, PA1 Department of Critical Care Medicine, University of Pittsburgh School of Medicine, Pittsburgh, PA1 Department of Critical Care Medicine, University of Pittsburgh School of Medicine, Pittsburgh, PA1 Department of Critical Care Medicine, University of Pittsburgh School of Medicine, Pittsburgh, PA4 Department of Chemistry, Vanderbilt University, Nashville, TN.4 Department of Chemistry, Vanderbilt University, Nashville, TN.5 Department of Surgery, UPMC, Pittsburgh, PA.5 Department of Surgery, UPMC, Pittsburgh, PA.6 Department of Pharmacology and Chemical Biology, University of Pittsburgh School of Medicine, Pittsburgh, PA.7 Department of Microbiology and Molecular Genetics, University of Pittsburgh School of Medicine, Pittsburgh, PA.8 Department of Biostatistics, University of Pittsburgh School of Public Health, Pittsburgh, PA.1 Department of Critical Care Medicine, University of Pittsburgh School of Medicine, Pittsburgh, PA2 Department of Critical Care Medicine, The CRISMA Center, University of Pittsburgh School of Medicine, Pittsburgh, PA.1 Department of Critical Care Medicine, University of Pittsburgh School of Medicine, Pittsburgh, PA9 Pulmonary, Allergy, and Critical Care Medicine Division, Center for Translational Lung Biology University of Pennsylvania Perelman School of Medicine, Philadelphia, PA.10 Division of Pulmonary and Critical Care Medicine, Department of Internal Medicine, University of Michigan Health System, Ann Arbor, MI.1 Department of Critical Care Medicine, University of Pittsburgh School of Medicine, Pittsburgh, PA1 Department of Critical Care Medicine, University of Pittsburgh School of Medicine, Pittsburgh, PABACKGROUND:. Sepsis is a common and deadly syndrome, accounting for more than 11 million deaths annually. To mature a deeper understanding of the host and pathogen mechanisms contributing to poor outcomes in sepsis, and thereby possibly inform new therapeutic targets, sophisticated, and expensive biorepositories are typically required. We propose that remnant biospecimens are an alternative for mechanistic sepsis research, although the viability and scientific value of such remnants are unknown. METHODS AND RESULTS:. The Remnant Biospecimen Investigation in Sepsis study is a prospective cohort study of 225 adults (age ≥ 18 yr) presenting to the emergency department with community sepsis, defined as sepsis-3 criteria within 6 hours of arrival. The primary objective was to determine the scientific value of a remnant biospecimen repository in sepsis linked to clinical phenotyping in the electronic health record. We will study candidate multiomic readouts of sepsis biology, governed by a conceptual model, and determine the precision, accuracy, integrity, and comparability of proteins, small molecules, lipids, and pathogen sequencing in remnant biospecimens compared with paired biospecimens obtained according to research protocols. Paired biospecimens will include plasma from sodium–heparin, EDTA, sodium fluoride, and citrate tubes. CONCLUSIONS:. The study has received approval from the University of Pittsburgh Human Research Protection Office (Study 21120013). Recruitment began on October 25, 2022, with planned release of primary results anticipated in 2024. Results will be made available to the public, the funders, critical care societies, laboratory medicine scientists, and other researchers.http://journals.lww.com/10.1097/CCE.0000000000000974 |
| spellingShingle | Christopher W. Seymour, MD, MSc Kelly Lynn Urbanek, MS Anna Nakayama Jason N. Kennedy, MS Rachel Powell, MD Renã A.S. Robinson, PhD Kathryn L. Kapp, BS Timothy R. Billiar, MD Yoram Vodovotz, PhD Stacy L. Gelhaus, PhD Vaughn S. Cooper, PhD Lu Tang, PhD Flo Mayr, MD Katherine M. Reitz, MD, MSc Christopher Horvat, MD Nuala J. Meyer, MD, MS Robert P. Dickson Derek Angus, MD Octavia Peck Palmer, PhD A Prospective Cohort Protocol for the Remnant Investigation in Sepsis Study Critical Care Explorations |
| title | A Prospective Cohort Protocol for the Remnant Investigation in Sepsis Study |
| title_full | A Prospective Cohort Protocol for the Remnant Investigation in Sepsis Study |
| title_fullStr | A Prospective Cohort Protocol for the Remnant Investigation in Sepsis Study |
| title_full_unstemmed | A Prospective Cohort Protocol for the Remnant Investigation in Sepsis Study |
| title_short | A Prospective Cohort Protocol for the Remnant Investigation in Sepsis Study |
| title_sort | prospective cohort protocol for the remnant investigation in sepsis study |
| url | http://journals.lww.com/10.1097/CCE.0000000000000974 |
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