Garlic tablet supplementation reduces lipopolysaccharide-induced TNF-alpha production by peripheral blood mononuclear cells
This study was designed to investigate whether garlic tablets possess anti-inflammatory and lipid-lowering effects in healthy adults. Twelve healthy adults participated in a randomized, cross-over design with a three-week treatment and a two-week washout period. Participants received either garlic p...
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| Format: | Article |
| Language: | English |
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SAGE Publishing
2016-12-01
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| Series: | European Journal of Inflammation |
| Online Access: | https://doi.org/10.1177/1721727X16675628 |
| _version_ | 1819262533522948096 |
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| author | Marjan Mahdavi-Roshan Marjan Rismanchi Javad Nasrollahzadeh |
| author_facet | Marjan Mahdavi-Roshan Marjan Rismanchi Javad Nasrollahzadeh |
| author_sort | Marjan Mahdavi-Roshan |
| collection | DOAJ |
| description | This study was designed to investigate whether garlic tablets possess anti-inflammatory and lipid-lowering effects in healthy adults. Twelve healthy adults participated in a randomized, cross-over design with a three-week treatment and a two-week washout period. Participants received either garlic powder tablets twice daily or two placebo tablets/day for three weeks. Plasma and peripheral blood mononuclear cells (PBMC) were isolated from fasting blood samples at baseline and after each three-week treatment with garlic or placebo. PBMC were cultured, stimulated with lipopolysaccharide (LPS), and changes in cell culture supernatants tumor necrosis factor-α (TNF-α) levels were determined. In addition, changes in plasma high-sensitivity C-reactive protein (hs-CRP), as well as plasma levels of lipids were determined. After three weeks of supplementation, LPS-stimulated TNF-α release in cell culture supernatant was lower after garlic than placebo ( P <0.05) whereas no significant changes were observed in unstimulated TNF-α release or plasma TNF-α. There were no significant differences in plasma hs-CRP, cholesterol, triglyceride, LDL cholesterol, and HDL cholesterol levels between garlic and placebo. In healthy individuals, garlic supplementation did not change plasma levels of TNF-α and hs-CRP while it caused lower TNF-α release into cell culture supernatant after stimulation by LPS. |
| first_indexed | 2024-12-23T19:59:13Z |
| format | Article |
| id | doaj.art-7ed2bf56f42e47cf943e4466786c2ba8 |
| institution | Directory Open Access Journal |
| issn | 1721-727X |
| language | English |
| last_indexed | 2024-12-23T19:59:13Z |
| publishDate | 2016-12-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | European Journal of Inflammation |
| spelling | doaj.art-7ed2bf56f42e47cf943e4466786c2ba82022-12-21T17:33:07ZengSAGE PublishingEuropean Journal of Inflammation1721-727X2016-12-011410.1177/1721727X16675628Garlic tablet supplementation reduces lipopolysaccharide-induced TNF-alpha production by peripheral blood mononuclear cellsMarjan Mahdavi-Roshan0Marjan Rismanchi1Javad Nasrollahzadeh2Department of Community Medicine; School of Medicine, Guilan University of Medical Science, Rasht, IranDepartment of Clinical Nutrition & Dietetics, National Nutrition, and Food Technology Research Institute, Faculty of Nutrition Sciences and Food Technology, Shahid Beheshti University of Medical Sciences, Tehran, IranDepartment of Clinical Nutrition & Dietetics, National Nutrition, and Food Technology Research Institute, Faculty of Nutrition Sciences and Food Technology, Shahid Beheshti University of Medical Sciences, Tehran, IranThis study was designed to investigate whether garlic tablets possess anti-inflammatory and lipid-lowering effects in healthy adults. Twelve healthy adults participated in a randomized, cross-over design with a three-week treatment and a two-week washout period. Participants received either garlic powder tablets twice daily or two placebo tablets/day for three weeks. Plasma and peripheral blood mononuclear cells (PBMC) were isolated from fasting blood samples at baseline and after each three-week treatment with garlic or placebo. PBMC were cultured, stimulated with lipopolysaccharide (LPS), and changes in cell culture supernatants tumor necrosis factor-α (TNF-α) levels were determined. In addition, changes in plasma high-sensitivity C-reactive protein (hs-CRP), as well as plasma levels of lipids were determined. After three weeks of supplementation, LPS-stimulated TNF-α release in cell culture supernatant was lower after garlic than placebo ( P <0.05) whereas no significant changes were observed in unstimulated TNF-α release or plasma TNF-α. There were no significant differences in plasma hs-CRP, cholesterol, triglyceride, LDL cholesterol, and HDL cholesterol levels between garlic and placebo. In healthy individuals, garlic supplementation did not change plasma levels of TNF-α and hs-CRP while it caused lower TNF-α release into cell culture supernatant after stimulation by LPS.https://doi.org/10.1177/1721727X16675628 |
| spellingShingle | Marjan Mahdavi-Roshan Marjan Rismanchi Javad Nasrollahzadeh Garlic tablet supplementation reduces lipopolysaccharide-induced TNF-alpha production by peripheral blood mononuclear cells European Journal of Inflammation |
| title | Garlic tablet supplementation reduces lipopolysaccharide-induced TNF-alpha production by peripheral blood mononuclear cells |
| title_full | Garlic tablet supplementation reduces lipopolysaccharide-induced TNF-alpha production by peripheral blood mononuclear cells |
| title_fullStr | Garlic tablet supplementation reduces lipopolysaccharide-induced TNF-alpha production by peripheral blood mononuclear cells |
| title_full_unstemmed | Garlic tablet supplementation reduces lipopolysaccharide-induced TNF-alpha production by peripheral blood mononuclear cells |
| title_short | Garlic tablet supplementation reduces lipopolysaccharide-induced TNF-alpha production by peripheral blood mononuclear cells |
| title_sort | garlic tablet supplementation reduces lipopolysaccharide induced tnf alpha production by peripheral blood mononuclear cells |
| url | https://doi.org/10.1177/1721727X16675628 |
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