A Caenorhabditis elegans locomotion phenotype caused by transgenic repeats of the hlh-17 promoter sequence.
Transgene technology is one of the most heavily relied upon tools in modern biological research. Expression of an exogenous gene within cells, for research and therapeutic applications, nearly always includes promoters and other regulatory sequences. We found that repeats of a non-protein coding tra...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2013-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3842965?pdf=render |
| _version_ | 1818131823465594880 |
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| author | Randy F Stout Vladimir Grubišić Vladimir Parpura |
| author_facet | Randy F Stout Vladimir Grubišić Vladimir Parpura |
| author_sort | Randy F Stout |
| collection | DOAJ |
| description | Transgene technology is one of the most heavily relied upon tools in modern biological research. Expression of an exogenous gene within cells, for research and therapeutic applications, nearly always includes promoters and other regulatory sequences. We found that repeats of a non-protein coding transgenic sequence produced profound changes to the behavior of the nematode Caenorhabditis elegans. These changes were produced by a glial promoter sequence but, unexpectedly, major deficits were observed specifically in backward locomotion, a neuron-driven behavior. We also present evidence that this behavioral phenotype is transpromoter copy number-dependent and manifests early in development and is maintained into adulthood of the worm. |
| first_indexed | 2024-12-11T08:27:03Z |
| format | Article |
| id | doaj.art-7ed541e8b3804b1a9688cf78240e341b |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-11T08:27:03Z |
| publishDate | 2013-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-7ed541e8b3804b1a9688cf78240e341b2022-12-22T01:14:32ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-01811e8177110.1371/journal.pone.0081771A Caenorhabditis elegans locomotion phenotype caused by transgenic repeats of the hlh-17 promoter sequence.Randy F StoutVladimir GrubišićVladimir ParpuraTransgene technology is one of the most heavily relied upon tools in modern biological research. Expression of an exogenous gene within cells, for research and therapeutic applications, nearly always includes promoters and other regulatory sequences. We found that repeats of a non-protein coding transgenic sequence produced profound changes to the behavior of the nematode Caenorhabditis elegans. These changes were produced by a glial promoter sequence but, unexpectedly, major deficits were observed specifically in backward locomotion, a neuron-driven behavior. We also present evidence that this behavioral phenotype is transpromoter copy number-dependent and manifests early in development and is maintained into adulthood of the worm.http://europepmc.org/articles/PMC3842965?pdf=render |
| spellingShingle | Randy F Stout Vladimir Grubišić Vladimir Parpura A Caenorhabditis elegans locomotion phenotype caused by transgenic repeats of the hlh-17 promoter sequence. PLoS ONE |
| title | A Caenorhabditis elegans locomotion phenotype caused by transgenic repeats of the hlh-17 promoter sequence. |
| title_full | A Caenorhabditis elegans locomotion phenotype caused by transgenic repeats of the hlh-17 promoter sequence. |
| title_fullStr | A Caenorhabditis elegans locomotion phenotype caused by transgenic repeats of the hlh-17 promoter sequence. |
| title_full_unstemmed | A Caenorhabditis elegans locomotion phenotype caused by transgenic repeats of the hlh-17 promoter sequence. |
| title_short | A Caenorhabditis elegans locomotion phenotype caused by transgenic repeats of the hlh-17 promoter sequence. |
| title_sort | caenorhabditis elegans locomotion phenotype caused by transgenic repeats of the hlh 17 promoter sequence |
| url | http://europepmc.org/articles/PMC3842965?pdf=render |
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