Biofilm stimulating activity of solanidine and Solasodine in Pseudomonas aeruginosa
Abstract Background Biofilm formation has reported as an important virulence associated properties of Pseudomonas aeruginosa that is regulated by quorum-sensing associated genes. Biofilm and quorum-sensing interfering properties of steroidal alkaloids, Solanidine and Solasodine were investigated in...
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| Format: | Article |
| Language: | English |
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BMC
2023-08-01
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| Series: | BMC Microbiology |
| Online Access: | https://doi.org/10.1186/s12866-023-02957-z |
| _version_ | 1797752844988710912 |
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| author | Hadi Ghoomdost Noori Omid Tadjrobehkar Elham Moazamian |
| author_facet | Hadi Ghoomdost Noori Omid Tadjrobehkar Elham Moazamian |
| author_sort | Hadi Ghoomdost Noori |
| collection | DOAJ |
| description | Abstract Background Biofilm formation has reported as an important virulence associated properties of Pseudomonas aeruginosa that is regulated by quorum-sensing associated genes. Biofilm and quorum-sensing interfering properties of steroidal alkaloids, Solanidine and Solasodine were investigated in the present study. Results Biofilm formation capacity and relative expression level of five studied genes(lasI, lasR, rhlI, rhlR and algD) were significantly increased dose-dependently after treatment with sub-inhibitory concentrations (32 and 512 µg/ml) of the both Solanidine and Solasodine. Biofilm formation capacity was more stimulated in weak biofilm formers(9 iaolates) in comparison to the strong biofilm producers(11 isolates). The lasI gene was the most induced QS-associated gene among five investigated genes. Conclusion Biofilm inducing properties of the plants alkaloids and probably medicines derived from them has to be considered for revision of therapeutic guidelines. Investigating the biofilm stimulating properties of corticosteroids and other medicines that comes from plant alkaloids also strongly proposed. |
| first_indexed | 2024-03-12T17:09:20Z |
| format | Article |
| id | doaj.art-7ed7a4f91cc64ea1abe42dbea660efd0 |
| institution | Directory Open Access Journal |
| issn | 1471-2180 |
| language | English |
| last_indexed | 2024-03-12T17:09:20Z |
| publishDate | 2023-08-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Microbiology |
| spelling | doaj.art-7ed7a4f91cc64ea1abe42dbea660efd02023-08-06T11:08:33ZengBMCBMC Microbiology1471-21802023-08-012311910.1186/s12866-023-02957-zBiofilm stimulating activity of solanidine and Solasodine in Pseudomonas aeruginosaHadi Ghoomdost Noori0Omid Tadjrobehkar1Elham Moazamian2Department of Microbiology, College of Sciences, Agriculture and Modern Technology, Shiraz Branch, Islamic Azad UniversityDepartment of Medical Microbiology (Bacteriology and Virology), Afzalipour Faculty of Medicine, Kerman University of Medical SciencesDepartment of Microbiology, College of Sciences, Agriculture and Modern Technology, Shiraz Branch, Islamic Azad UniversityAbstract Background Biofilm formation has reported as an important virulence associated properties of Pseudomonas aeruginosa that is regulated by quorum-sensing associated genes. Biofilm and quorum-sensing interfering properties of steroidal alkaloids, Solanidine and Solasodine were investigated in the present study. Results Biofilm formation capacity and relative expression level of five studied genes(lasI, lasR, rhlI, rhlR and algD) were significantly increased dose-dependently after treatment with sub-inhibitory concentrations (32 and 512 µg/ml) of the both Solanidine and Solasodine. Biofilm formation capacity was more stimulated in weak biofilm formers(9 iaolates) in comparison to the strong biofilm producers(11 isolates). The lasI gene was the most induced QS-associated gene among five investigated genes. Conclusion Biofilm inducing properties of the plants alkaloids and probably medicines derived from them has to be considered for revision of therapeutic guidelines. Investigating the biofilm stimulating properties of corticosteroids and other medicines that comes from plant alkaloids also strongly proposed.https://doi.org/10.1186/s12866-023-02957-z |
| spellingShingle | Hadi Ghoomdost Noori Omid Tadjrobehkar Elham Moazamian Biofilm stimulating activity of solanidine and Solasodine in Pseudomonas aeruginosa BMC Microbiology |
| title | Biofilm stimulating activity of solanidine and Solasodine in Pseudomonas aeruginosa |
| title_full | Biofilm stimulating activity of solanidine and Solasodine in Pseudomonas aeruginosa |
| title_fullStr | Biofilm stimulating activity of solanidine and Solasodine in Pseudomonas aeruginosa |
| title_full_unstemmed | Biofilm stimulating activity of solanidine and Solasodine in Pseudomonas aeruginosa |
| title_short | Biofilm stimulating activity of solanidine and Solasodine in Pseudomonas aeruginosa |
| title_sort | biofilm stimulating activity of solanidine and solasodine in pseudomonas aeruginosa |
| url | https://doi.org/10.1186/s12866-023-02957-z |
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