Development of a bead-based assay for detection of three banana-infecting viruses
Background Banana bunchy top virus (BBTV), cucumber mosaic virus (CMV) and banana streak virus (BSV) are important banana viruses, there are possible infections frequently with several viruses in field. Since the viruses are readily trasmitted in vegetative propagules, which pose a threat to banana...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
PeerJ Inc.
2022-05-01
|
| Series: | PeerJ |
| Subjects: | |
| Online Access: | https://peerj.com/articles/13409.pdf |
| _version_ | 1827606722052620288 |
|---|---|
| author | Cheng-Ping Kuan Chia-Hsin Tsai Ching-Shan Tseng Tso-Chi Yang |
| author_facet | Cheng-Ping Kuan Chia-Hsin Tsai Ching-Shan Tseng Tso-Chi Yang |
| author_sort | Cheng-Ping Kuan |
| collection | DOAJ |
| description | Background Banana bunchy top virus (BBTV), cucumber mosaic virus (CMV) and banana streak virus (BSV) are important banana viruses, there are possible infections frequently with several viruses in field. Since the viruses are readily trasmitted in vegetative propagules, which pose a threat to banana production in banana-growing areas. Methods A multiplex polymerase chain reaction (PCR) protocol combined with LiquiChip analysis to identify BSV, BBTV, and CMV, with consistent amplification of plant ubiquitin (UBQ), the banana plant messenger RNA used as a procedural control. Multiplex reverse transcription (RT)-PCR amplicons were extended by allele-specific primers, followed by hybridization with carboxylated microspheres containing unique fluorescent oligonucleotides, which were detected using the LiquiChip 200 workstation. Results In this study, we aimed to develop a rapid, sensitive, and simultaneous detection method for BSV, BBTV, and CMV using a bead-based multiplex assay that can be applied in routine diagnosis. We demonstrated that this detection system was extremely efficient and highly specialized for differentiating individual in a mixture of viruses while being ten times more sensitive than traditional RT-PCR. The development of this method makes it feasible to detect banana viruses in field collected leaf samples. |
| first_indexed | 2024-03-09T06:42:28Z |
| format | Article |
| id | doaj.art-7ed7a793e6844d8da544539c7f3a1a5e |
| institution | Directory Open Access Journal |
| issn | 2167-8359 |
| language | English |
| last_indexed | 2024-03-09T06:42:28Z |
| publishDate | 2022-05-01 |
| publisher | PeerJ Inc. |
| record_format | Article |
| series | PeerJ |
| spelling | doaj.art-7ed7a793e6844d8da544539c7f3a1a5e2023-12-03T10:47:37ZengPeerJ Inc.PeerJ2167-83592022-05-0110e1340910.7717/peerj.13409Development of a bead-based assay for detection of three banana-infecting virusesCheng-Ping Kuan0Chia-Hsin Tsai1Ching-Shan Tseng2Tso-Chi Yang3Division of Biotechnology, Taiwan Agricultural Research Institute, Taichung, TaiwanDivision of Plant Pathology, Taiwan Agricultural Research Institute, Taichung, TaiwanDivision of Biotechnology, Taiwan Agricultural Research Institute, Taichung, TaiwanDivision of Biotechnology, Taiwan Agricultural Research Institute, Taichung, TaiwanBackground Banana bunchy top virus (BBTV), cucumber mosaic virus (CMV) and banana streak virus (BSV) are important banana viruses, there are possible infections frequently with several viruses in field. Since the viruses are readily trasmitted in vegetative propagules, which pose a threat to banana production in banana-growing areas. Methods A multiplex polymerase chain reaction (PCR) protocol combined with LiquiChip analysis to identify BSV, BBTV, and CMV, with consistent amplification of plant ubiquitin (UBQ), the banana plant messenger RNA used as a procedural control. Multiplex reverse transcription (RT)-PCR amplicons were extended by allele-specific primers, followed by hybridization with carboxylated microspheres containing unique fluorescent oligonucleotides, which were detected using the LiquiChip 200 workstation. Results In this study, we aimed to develop a rapid, sensitive, and simultaneous detection method for BSV, BBTV, and CMV using a bead-based multiplex assay that can be applied in routine diagnosis. We demonstrated that this detection system was extremely efficient and highly specialized for differentiating individual in a mixture of viruses while being ten times more sensitive than traditional RT-PCR. The development of this method makes it feasible to detect banana viruses in field collected leaf samples.https://peerj.com/articles/13409.pdfBanana bunchy top virusBanana streak virusCucumber mosaic virusBananaDetection |
| spellingShingle | Cheng-Ping Kuan Chia-Hsin Tsai Ching-Shan Tseng Tso-Chi Yang Development of a bead-based assay for detection of three banana-infecting viruses PeerJ Banana bunchy top virus Banana streak virus Cucumber mosaic virus Banana Detection |
| title | Development of a bead-based assay for detection of three banana-infecting viruses |
| title_full | Development of a bead-based assay for detection of three banana-infecting viruses |
| title_fullStr | Development of a bead-based assay for detection of three banana-infecting viruses |
| title_full_unstemmed | Development of a bead-based assay for detection of three banana-infecting viruses |
| title_short | Development of a bead-based assay for detection of three banana-infecting viruses |
| title_sort | development of a bead based assay for detection of three banana infecting viruses |
| topic | Banana bunchy top virus Banana streak virus Cucumber mosaic virus Banana Detection |
| url | https://peerj.com/articles/13409.pdf |
| work_keys_str_mv | AT chengpingkuan developmentofabeadbasedassayfordetectionofthreebananainfectingviruses AT chiahsintsai developmentofabeadbasedassayfordetectionofthreebananainfectingviruses AT chingshantseng developmentofabeadbasedassayfordetectionofthreebananainfectingviruses AT tsochiyang developmentofabeadbasedassayfordetectionofthreebananainfectingviruses |