Nanosphere's Verigene® Blood Culture Assay to Detect Multidrug-Resistant Gram-Negative Bacterial Outbreak: A Prospective Study on 79 Hematological Patients in a Country with High Prevalence of Antimicrobial Resistance
Infections are a major cause of morbidity and mortality in hematological patients. We prospectively tested a new molecular assay (Verigene®) in 79 consecutive hematological patients, with sepsis by gram-negative bacteria. A total of 82 gram-negative microorganisms were isolated by blood cultures, of...
| Main Authors: | , , , , , , , , , , , , , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
SAABRON PRESS
2019-06-01
|
| Series: | Clinical Hematology International |
| Subjects: | |
| Online Access: | https://www.atlantis-press.com/article/125907527/view |
| _version_ | 1797229387301519360 |
|---|---|
| author | Raffaella Greco Maria Chiara Barbanti Nicasio Mancini Laura Infurnari Renée Pasciuta Alessandra Forcina Chiara Oltolini Gabriele Casirati Daniele Mannina Fabio Giglio Carlo Messina Mara Morelli Francesca Lorentino Sara Mastaglio Tommaso Perini Luca Vago Paolo Scarpellini Matteo Giovanni Carrabba Maria Teresa Lupo Stanghellini Sarah Marktel Andrea Assanelli Massimo Bernardi Consuelo Corti Jacopo Peccatori Massimo Clementi Fabio Ciceri |
| author_facet | Raffaella Greco Maria Chiara Barbanti Nicasio Mancini Laura Infurnari Renée Pasciuta Alessandra Forcina Chiara Oltolini Gabriele Casirati Daniele Mannina Fabio Giglio Carlo Messina Mara Morelli Francesca Lorentino Sara Mastaglio Tommaso Perini Luca Vago Paolo Scarpellini Matteo Giovanni Carrabba Maria Teresa Lupo Stanghellini Sarah Marktel Andrea Assanelli Massimo Bernardi Consuelo Corti Jacopo Peccatori Massimo Clementi Fabio Ciceri |
| author_sort | Raffaella Greco |
| collection | DOAJ |
| description | Infections are a major cause of morbidity and mortality in hematological patients. We prospectively tested a new molecular assay (Verigene®) in 79 consecutive hematological patients, with sepsis by gram-negative bacteria. A total of 82 gram-negative microorganisms were isolated by blood cultures, of which 76 cases were mono-microbial. Considering the bacteria detectable by the system, the concordance with standard blood cultures was 100%. Resistance genes were detected in 20 of the isolates and 100% were concordant with the phenotypic antibiotic resistance. Overall, this new assay correctly identified 66/82 of all the gram-negative pathogens, yielding a general sensitivity of 80.5%, and providing information on genetic antibiotic resistance in a few hours. This new molecular assay could ameliorate patient management, resulting in a more rational use of antibiotics. |
| first_indexed | 2024-04-24T15:11:47Z |
| format | Article |
| id | doaj.art-7ed8670b646b4b228c6da20932894498 |
| institution | Directory Open Access Journal |
| issn | 2590-0048 |
| language | English |
| last_indexed | 2024-04-24T15:11:47Z |
| publishDate | 2019-06-01 |
| publisher | SAABRON PRESS |
| record_format | Article |
| series | Clinical Hematology International |
| spelling | doaj.art-7ed8670b646b4b228c6da209328944982024-04-02T10:49:23ZengSAABRON PRESSClinical Hematology International2590-00482019-06-011210.2991/chi.d.190321.001Nanosphere's Verigene® Blood Culture Assay to Detect Multidrug-Resistant Gram-Negative Bacterial Outbreak: A Prospective Study on 79 Hematological Patients in a Country with High Prevalence of Antimicrobial ResistanceRaffaella GrecoMaria Chiara BarbantiNicasio ManciniLaura InfurnariRenée PasciutaAlessandra ForcinaChiara OltoliniGabriele CasiratiDaniele ManninaFabio GiglioCarlo MessinaMara MorelliFrancesca LorentinoSara MastaglioTommaso PeriniLuca VagoPaolo ScarpelliniMatteo Giovanni CarrabbaMaria Teresa Lupo StanghelliniSarah MarktelAndrea AssanelliMassimo BernardiConsuelo CortiJacopo PeccatoriMassimo ClementiFabio CiceriInfections are a major cause of morbidity and mortality in hematological patients. We prospectively tested a new molecular assay (Verigene®) in 79 consecutive hematological patients, with sepsis by gram-negative bacteria. A total of 82 gram-negative microorganisms were isolated by blood cultures, of which 76 cases were mono-microbial. Considering the bacteria detectable by the system, the concordance with standard blood cultures was 100%. Resistance genes were detected in 20 of the isolates and 100% were concordant with the phenotypic antibiotic resistance. Overall, this new assay correctly identified 66/82 of all the gram-negative pathogens, yielding a general sensitivity of 80.5%, and providing information on genetic antibiotic resistance in a few hours. This new molecular assay could ameliorate patient management, resulting in a more rational use of antibiotics.https://www.atlantis-press.com/article/125907527/viewSepsisHematological patientsMolecular diagnosisCarbapenem resistance |
| spellingShingle | Raffaella Greco Maria Chiara Barbanti Nicasio Mancini Laura Infurnari Renée Pasciuta Alessandra Forcina Chiara Oltolini Gabriele Casirati Daniele Mannina Fabio Giglio Carlo Messina Mara Morelli Francesca Lorentino Sara Mastaglio Tommaso Perini Luca Vago Paolo Scarpellini Matteo Giovanni Carrabba Maria Teresa Lupo Stanghellini Sarah Marktel Andrea Assanelli Massimo Bernardi Consuelo Corti Jacopo Peccatori Massimo Clementi Fabio Ciceri Nanosphere's Verigene® Blood Culture Assay to Detect Multidrug-Resistant Gram-Negative Bacterial Outbreak: A Prospective Study on 79 Hematological Patients in a Country with High Prevalence of Antimicrobial Resistance Clinical Hematology International Sepsis Hematological patients Molecular diagnosis Carbapenem resistance |
| title | Nanosphere's Verigene® Blood Culture Assay to Detect Multidrug-Resistant Gram-Negative Bacterial Outbreak: A Prospective Study on 79 Hematological Patients in a Country with High Prevalence of Antimicrobial Resistance |
| title_full | Nanosphere's Verigene® Blood Culture Assay to Detect Multidrug-Resistant Gram-Negative Bacterial Outbreak: A Prospective Study on 79 Hematological Patients in a Country with High Prevalence of Antimicrobial Resistance |
| title_fullStr | Nanosphere's Verigene® Blood Culture Assay to Detect Multidrug-Resistant Gram-Negative Bacterial Outbreak: A Prospective Study on 79 Hematological Patients in a Country with High Prevalence of Antimicrobial Resistance |
| title_full_unstemmed | Nanosphere's Verigene® Blood Culture Assay to Detect Multidrug-Resistant Gram-Negative Bacterial Outbreak: A Prospective Study on 79 Hematological Patients in a Country with High Prevalence of Antimicrobial Resistance |
| title_short | Nanosphere's Verigene® Blood Culture Assay to Detect Multidrug-Resistant Gram-Negative Bacterial Outbreak: A Prospective Study on 79 Hematological Patients in a Country with High Prevalence of Antimicrobial Resistance |
| title_sort | nanosphere s verigene r blood culture assay to detect multidrug resistant gram negative bacterial outbreak a prospective study on 79 hematological patients in a country with high prevalence of antimicrobial resistance |
| topic | Sepsis Hematological patients Molecular diagnosis Carbapenem resistance |
| url | https://www.atlantis-press.com/article/125907527/view |
| work_keys_str_mv | AT raffaellagreco nanospheresverigenebloodcultureassaytodetectmultidrugresistantgramnegativebacterialoutbreakaprospectivestudyon79hematologicalpatientsinacountrywithhighprevalenceofantimicrobialresistance AT mariachiarabarbanti nanospheresverigenebloodcultureassaytodetectmultidrugresistantgramnegativebacterialoutbreakaprospectivestudyon79hematologicalpatientsinacountrywithhighprevalenceofantimicrobialresistance AT nicasiomancini nanospheresverigenebloodcultureassaytodetectmultidrugresistantgramnegativebacterialoutbreakaprospectivestudyon79hematologicalpatientsinacountrywithhighprevalenceofantimicrobialresistance AT laurainfurnari nanospheresverigenebloodcultureassaytodetectmultidrugresistantgramnegativebacterialoutbreakaprospectivestudyon79hematologicalpatientsinacountrywithhighprevalenceofantimicrobialresistance AT reneepasciuta nanospheresverigenebloodcultureassaytodetectmultidrugresistantgramnegativebacterialoutbreakaprospectivestudyon79hematologicalpatientsinacountrywithhighprevalenceofantimicrobialresistance AT alessandraforcina nanospheresverigenebloodcultureassaytodetectmultidrugresistantgramnegativebacterialoutbreakaprospectivestudyon79hematologicalpatientsinacountrywithhighprevalenceofantimicrobialresistance AT chiaraoltolini nanospheresverigenebloodcultureassaytodetectmultidrugresistantgramnegativebacterialoutbreakaprospectivestudyon79hematologicalpatientsinacountrywithhighprevalenceofantimicrobialresistance AT gabrielecasirati nanospheresverigenebloodcultureassaytodetectmultidrugresistantgramnegativebacterialoutbreakaprospectivestudyon79hematologicalpatientsinacountrywithhighprevalenceofantimicrobialresistance AT danielemannina nanospheresverigenebloodcultureassaytodetectmultidrugresistantgramnegativebacterialoutbreakaprospectivestudyon79hematologicalpatientsinacountrywithhighprevalenceofantimicrobialresistance AT fabiogiglio nanospheresverigenebloodcultureassaytodetectmultidrugresistantgramnegativebacterialoutbreakaprospectivestudyon79hematologicalpatientsinacountrywithhighprevalenceofantimicrobialresistance AT carlomessina nanospheresverigenebloodcultureassaytodetectmultidrugresistantgramnegativebacterialoutbreakaprospectivestudyon79hematologicalpatientsinacountrywithhighprevalenceofantimicrobialresistance AT maramorelli nanospheresverigenebloodcultureassaytodetectmultidrugresistantgramnegativebacterialoutbreakaprospectivestudyon79hematologicalpatientsinacountrywithhighprevalenceofantimicrobialresistance AT francescalorentino nanospheresverigenebloodcultureassaytodetectmultidrugresistantgramnegativebacterialoutbreakaprospectivestudyon79hematologicalpatientsinacountrywithhighprevalenceofantimicrobialresistance AT saramastaglio nanospheresverigenebloodcultureassaytodetectmultidrugresistantgramnegativebacterialoutbreakaprospectivestudyon79hematologicalpatientsinacountrywithhighprevalenceofantimicrobialresistance AT tommasoperini nanospheresverigenebloodcultureassaytodetectmultidrugresistantgramnegativebacterialoutbreakaprospectivestudyon79hematologicalpatientsinacountrywithhighprevalenceofantimicrobialresistance AT lucavago nanospheresverigenebloodcultureassaytodetectmultidrugresistantgramnegativebacterialoutbreakaprospectivestudyon79hematologicalpatientsinacountrywithhighprevalenceofantimicrobialresistance AT paoloscarpellini nanospheresverigenebloodcultureassaytodetectmultidrugresistantgramnegativebacterialoutbreakaprospectivestudyon79hematologicalpatientsinacountrywithhighprevalenceofantimicrobialresistance AT matteogiovannicarrabba nanospheresverigenebloodcultureassaytodetectmultidrugresistantgramnegativebacterialoutbreakaprospectivestudyon79hematologicalpatientsinacountrywithhighprevalenceofantimicrobialresistance AT mariateresalupostanghellini nanospheresverigenebloodcultureassaytodetectmultidrugresistantgramnegativebacterialoutbreakaprospectivestudyon79hematologicalpatientsinacountrywithhighprevalenceofantimicrobialresistance AT sarahmarktel nanospheresverigenebloodcultureassaytodetectmultidrugresistantgramnegativebacterialoutbreakaprospectivestudyon79hematologicalpatientsinacountrywithhighprevalenceofantimicrobialresistance AT andreaassanelli nanospheresverigenebloodcultureassaytodetectmultidrugresistantgramnegativebacterialoutbreakaprospectivestudyon79hematologicalpatientsinacountrywithhighprevalenceofantimicrobialresistance AT massimobernardi nanospheresverigenebloodcultureassaytodetectmultidrugresistantgramnegativebacterialoutbreakaprospectivestudyon79hematologicalpatientsinacountrywithhighprevalenceofantimicrobialresistance AT consuelocorti nanospheresverigenebloodcultureassaytodetectmultidrugresistantgramnegativebacterialoutbreakaprospectivestudyon79hematologicalpatientsinacountrywithhighprevalenceofantimicrobialresistance AT jacopopeccatori nanospheresverigenebloodcultureassaytodetectmultidrugresistantgramnegativebacterialoutbreakaprospectivestudyon79hematologicalpatientsinacountrywithhighprevalenceofantimicrobialresistance AT massimoclementi nanospheresverigenebloodcultureassaytodetectmultidrugresistantgramnegativebacterialoutbreakaprospectivestudyon79hematologicalpatientsinacountrywithhighprevalenceofantimicrobialresistance AT fabiociceri nanospheresverigenebloodcultureassaytodetectmultidrugresistantgramnegativebacterialoutbreakaprospectivestudyon79hematologicalpatientsinacountrywithhighprevalenceofantimicrobialresistance |