Diagnostic Value of Direct Antibiotic Susceptibility Test for Faster BacterialSusceptibility Reporting in Bacteremia
Background: Rapid and accurate information on susceptibility of bacteria causing bacteraemia is very helpful in sepsis management. Blood culture is the gold standard for bacteraemia diagnosis. Standard antibiotic susceptibility testing needs at least three days for completion while direct method can...
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| Format: | Article |
| Language: | English |
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Universitas Sultan Agung Semarang
2012-12-01
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| Series: | Sains Medika |
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| Online Access: | http://jurnal.unissula.ac.id/index.php/sainsmedika/article/view/374 |
| _version_ | 1818319910639501312 |
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| author | Rebriarina Hapsari Vincentia Rizke Ciptaningtyas Masfiyah Masfiyah |
| author_facet | Rebriarina Hapsari Vincentia Rizke Ciptaningtyas Masfiyah Masfiyah |
| author_sort | Rebriarina Hapsari |
| collection | DOAJ |
| description | Background: Rapid and accurate information on susceptibility of bacteria causing bacteraemia is very helpful in sepsis management. Blood culture is the gold standard for bacteraemia diagnosis. Standard antibiotic susceptibility testing needs at least three days for completion while direct method can give the result a day earlier. The aim of this study was to investigate the diagnostic value of direct antibiotic susceptibility testing in blood culture.
Methods: Bloods from positive BACTEC bottles which met inclusion and exclusion criteria were put into sterile tubes and centrifuged. The pellets were then used to make 0.5 McFarland bacterial suspensions and directly used for antibiotic susceptibility testing. Interpretations of direct method were compared to standard method to count sensitivity, specificity, sensitive predictive value, resistant predictive value, accuracy, and kappa value.
Results: From 58 samples (containing 22 gram negative, 36 gram positive bacteria), there were 309 total antibiotic susceptibility tests. Direct method showed sensitivity, specificity, sensitive predictive value, resistant predictive value, accuracy, and kappa value of 89.3%, 92.9%, 93.8%, 87.8%, 86.4%, and 0.82, respectively.
Conclusion: Direct antibiotic susceptibility testing has a good agreement with the standard method so it can aid faster antibiotic susceptibility reporting in bacteraemia (Sains Medika, 4(2):174-181). |
| first_indexed | 2024-12-13T10:16:37Z |
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| id | doaj.art-7ed8e5b0a457453d990c77f229f28d1f |
| institution | Directory Open Access Journal |
| issn | 2085-1545 2339-093X |
| language | English |
| last_indexed | 2024-12-13T10:16:37Z |
| publishDate | 2012-12-01 |
| publisher | Universitas Sultan Agung Semarang |
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| series | Sains Medika |
| spelling | doaj.art-7ed8e5b0a457453d990c77f229f28d1f2022-12-21T23:51:17ZengUniversitas Sultan Agung SemarangSains Medika2085-15452339-093X2012-12-014217418110.26532/sainsmed.v4i2.374370Diagnostic Value of Direct Antibiotic Susceptibility Test for Faster BacterialSusceptibility Reporting in BacteremiaRebriarina Hapsari0Vincentia Rizke Ciptaningtyas1Masfiyah Masfiyah2Bagian Mikrobiologi Fakultas Kedokteran Universitas Diponegoro Semarang/ RSUP Dr Kariadi SemarangBagian Mikrobiologi Fakultas Kedokteran Universitas Diponegoro Semarang/ RSUP Dr Kariadi SemarangBagian Mikrobiologi Fakultas Kedokteran Universitas Diponegoro Semarang/ RSUP Dr Kariadi Semarang Fakultas Kedokteran Unissula Semarang/ RS Islam Sultan Agung SemarangBackground: Rapid and accurate information on susceptibility of bacteria causing bacteraemia is very helpful in sepsis management. Blood culture is the gold standard for bacteraemia diagnosis. Standard antibiotic susceptibility testing needs at least three days for completion while direct method can give the result a day earlier. The aim of this study was to investigate the diagnostic value of direct antibiotic susceptibility testing in blood culture. Methods: Bloods from positive BACTEC bottles which met inclusion and exclusion criteria were put into sterile tubes and centrifuged. The pellets were then used to make 0.5 McFarland bacterial suspensions and directly used for antibiotic susceptibility testing. Interpretations of direct method were compared to standard method to count sensitivity, specificity, sensitive predictive value, resistant predictive value, accuracy, and kappa value. Results: From 58 samples (containing 22 gram negative, 36 gram positive bacteria), there were 309 total antibiotic susceptibility tests. Direct method showed sensitivity, specificity, sensitive predictive value, resistant predictive value, accuracy, and kappa value of 89.3%, 92.9%, 93.8%, 87.8%, 86.4%, and 0.82, respectively. Conclusion: Direct antibiotic susceptibility testing has a good agreement with the standard method so it can aid faster antibiotic susceptibility reporting in bacteraemia (Sains Medika, 4(2):174-181).http://jurnal.unissula.ac.id/index.php/sainsmedika/article/view/374bacteraemiablood cultureantibiotic susceptibility testing |
| spellingShingle | Rebriarina Hapsari Vincentia Rizke Ciptaningtyas Masfiyah Masfiyah Diagnostic Value of Direct Antibiotic Susceptibility Test for Faster BacterialSusceptibility Reporting in Bacteremia Sains Medika bacteraemia blood culture antibiotic susceptibility testing |
| title | Diagnostic Value of Direct Antibiotic Susceptibility Test for Faster BacterialSusceptibility Reporting in Bacteremia |
| title_full | Diagnostic Value of Direct Antibiotic Susceptibility Test for Faster BacterialSusceptibility Reporting in Bacteremia |
| title_fullStr | Diagnostic Value of Direct Antibiotic Susceptibility Test for Faster BacterialSusceptibility Reporting in Bacteremia |
| title_full_unstemmed | Diagnostic Value of Direct Antibiotic Susceptibility Test for Faster BacterialSusceptibility Reporting in Bacteremia |
| title_short | Diagnostic Value of Direct Antibiotic Susceptibility Test for Faster BacterialSusceptibility Reporting in Bacteremia |
| title_sort | diagnostic value of direct antibiotic susceptibility test for faster bacterialsusceptibility reporting in bacteremia |
| topic | bacteraemia blood culture antibiotic susceptibility testing |
| url | http://jurnal.unissula.ac.id/index.php/sainsmedika/article/view/374 |
| work_keys_str_mv | AT rebriarinahapsari diagnosticvalueofdirectantibioticsusceptibilitytestforfasterbacterialsusceptibilityreportinginbacteremia AT vincentiarizkeciptaningtyas diagnosticvalueofdirectantibioticsusceptibilitytestforfasterbacterialsusceptibilityreportinginbacteremia AT masfiyahmasfiyah diagnosticvalueofdirectantibioticsusceptibilitytestforfasterbacterialsusceptibilityreportinginbacteremia |