Genetic Polymorphism and Association of KAP16.6 Gene with Pashmina Fiber Traits in Changthangi Goats
Pashmina is the fine undercoat of the Changthangi goats (Capra hircus) with Keratin-associated proteins (KAPs) as one of its main structural components. KAPs form a semi-rigid matrix in which the Keratin intermediate filaments (KIFs) are embedded. In the present study, an attempt was made to elucida...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2022-08-01
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| Series: | Journal of Natural Fibers |
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| Online Access: | http://dx.doi.org/10.1080/15440478.2020.1821296 |
| _version_ | 1827812958541971456 |
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| author | Syed Shanaz Tasleem Ahmad Shamas Ganai Sheikh Firdous Ahmad Ishraq Hussain Azmat Aalam Khan Nazir Ahmad Ganai |
| author_facet | Syed Shanaz Tasleem Ahmad Shamas Ganai Sheikh Firdous Ahmad Ishraq Hussain Azmat Aalam Khan Nazir Ahmad Ganai |
| author_sort | Syed Shanaz |
| collection | DOAJ |
| description | Pashmina is the fine undercoat of the Changthangi goats (Capra hircus) with Keratin-associated proteins (KAPs) as one of its main structural components. KAPs form a semi-rigid matrix in which the Keratin intermediate filaments (KIFs) are embedded. In the present study, an attempt was made to elucidate the polymorphism of KAP16.6 gene loci and its association with fiber yield and quality traits (fine yield, mean fiber diameter, mean fiber length, and guard hair percentage). A total of 103 animals of Changthangi goats were randomly selected and genomic DNA was isolated from blood samples. After amplification of the coding region of KAP16.6 (254 bp) gene, restriction digestion with HindIII enzyme (AAG/CTT) produced two polymorphic patterns in the Changthangi goats. The genotypic distribution of the designated genotypes A1A1 (0.0), A2A2 (0.379), and A1A2 (0.621) of KAP16.6 gene varied significantly (p < .01) from Hardy-Weinberg equilibrium. Missense mutation AGC (Serine) > AAC (Asparagine) was identified at KAP16.6 locus in Changthangi goats for the first time. The genetic variability parameters for the gene loci analyzed were: heterozygosity value 0.4283, PIC: 0.3366, effective allele no 1.7492 and Shannon index (I) of 0.6196. The estimated PIC value reflected median polymorphism exhibited by the Changthangi goat population under study for the selected gene. Statistical analysis further revealed a significant effect of KAP16.6 genotype on the fiber length (p < .05) whereas the effect was nonsignificant for other Pashmina production traits under study. The results from the present study have practical significance in the breeding plans for Changthangi goats in this region for improved production performance. |
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| issn | 1544-0478 1544-046X |
| language | English |
| last_indexed | 2024-03-11T23:25:40Z |
| publishDate | 2022-08-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Journal of Natural Fibers |
| spelling | doaj.art-7edc181f20a34639ab4514fa196b83d02023-09-20T12:50:21ZengTaylor & Francis GroupJournal of Natural Fibers1544-04781544-046X2022-08-011982813282210.1080/15440478.2020.18212961821296Genetic Polymorphism and Association of KAP16.6 Gene with Pashmina Fiber Traits in Changthangi GoatsSyed Shanaz0Tasleem Ahmad Shamas Ganai1Sheikh Firdous Ahmad2Ishraq Hussain3Azmat Aalam Khan4Nazir Ahmad Ganai5Division of Animal Genetics and BreedingDivision of Animal Genetics and BreedingICAR-National Research Centre on PigDivision of Veterinary BiochemistrySher-e-Kashmir University of Agricultural Sciences and TechnologyDivision of Animal Genetics and BreedingPashmina is the fine undercoat of the Changthangi goats (Capra hircus) with Keratin-associated proteins (KAPs) as one of its main structural components. KAPs form a semi-rigid matrix in which the Keratin intermediate filaments (KIFs) are embedded. In the present study, an attempt was made to elucidate the polymorphism of KAP16.6 gene loci and its association with fiber yield and quality traits (fine yield, mean fiber diameter, mean fiber length, and guard hair percentage). A total of 103 animals of Changthangi goats were randomly selected and genomic DNA was isolated from blood samples. After amplification of the coding region of KAP16.6 (254 bp) gene, restriction digestion with HindIII enzyme (AAG/CTT) produced two polymorphic patterns in the Changthangi goats. The genotypic distribution of the designated genotypes A1A1 (0.0), A2A2 (0.379), and A1A2 (0.621) of KAP16.6 gene varied significantly (p < .01) from Hardy-Weinberg equilibrium. Missense mutation AGC (Serine) > AAC (Asparagine) was identified at KAP16.6 locus in Changthangi goats for the first time. The genetic variability parameters for the gene loci analyzed were: heterozygosity value 0.4283, PIC: 0.3366, effective allele no 1.7492 and Shannon index (I) of 0.6196. The estimated PIC value reflected median polymorphism exhibited by the Changthangi goat population under study for the selected gene. Statistical analysis further revealed a significant effect of KAP16.6 genotype on the fiber length (p < .05) whereas the effect was nonsignificant for other Pashmina production traits under study. The results from the present study have practical significance in the breeding plans for Changthangi goats in this region for improved production performance.http://dx.doi.org/10.1080/15440478.2020.1821296characterizationdiversitypashminapolymorphismsequence |
| spellingShingle | Syed Shanaz Tasleem Ahmad Shamas Ganai Sheikh Firdous Ahmad Ishraq Hussain Azmat Aalam Khan Nazir Ahmad Ganai Genetic Polymorphism and Association of KAP16.6 Gene with Pashmina Fiber Traits in Changthangi Goats Journal of Natural Fibers characterization diversity pashmina polymorphism sequence |
| title | Genetic Polymorphism and Association of KAP16.6 Gene with Pashmina Fiber Traits in Changthangi Goats |
| title_full | Genetic Polymorphism and Association of KAP16.6 Gene with Pashmina Fiber Traits in Changthangi Goats |
| title_fullStr | Genetic Polymorphism and Association of KAP16.6 Gene with Pashmina Fiber Traits in Changthangi Goats |
| title_full_unstemmed | Genetic Polymorphism and Association of KAP16.6 Gene with Pashmina Fiber Traits in Changthangi Goats |
| title_short | Genetic Polymorphism and Association of KAP16.6 Gene with Pashmina Fiber Traits in Changthangi Goats |
| title_sort | genetic polymorphism and association of kap16 6 gene with pashmina fiber traits in changthangi goats |
| topic | characterization diversity pashmina polymorphism sequence |
| url | http://dx.doi.org/10.1080/15440478.2020.1821296 |
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