Novel Antimicrobial Peptide from the Hepatopancreas of the Red King Crab
Crustaceans have successfully adapted to survive in their natural habitat, rich in microorganisms, due to the presence of antimicrobial peptides (AMPs) in their organism. They achieve this adaptation despite lacking the highly specific adaptive immune system found in vertebrates. One valuable source...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-10-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/21/15607 |
| _version_ | 1797631867630911488 |
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| author | Vladislav Molchanov Alexander Yegorov Maxim Molchanov Alexander Timchenko Vitaly Novikov Nikolay Novojilov Maria Timchenko |
| author_facet | Vladislav Molchanov Alexander Yegorov Maxim Molchanov Alexander Timchenko Vitaly Novikov Nikolay Novojilov Maria Timchenko |
| author_sort | Vladislav Molchanov |
| collection | DOAJ |
| description | Crustaceans have successfully adapted to survive in their natural habitat, rich in microorganisms, due to the presence of antimicrobial peptides (AMPs) in their organism. They achieve this adaptation despite lacking the highly specific adaptive immune system found in vertebrates. One valuable source of AMPs is the hepatopancreas, a waste product from crab fishery and its processing. Applying zymographic and spectrophotometric techniques, we discovered a small peptide (approximately 5 kDa) within a low molecular weight protein fraction extracted from the acetone powder of the red king crab hepatopancreas. This peptide hydrolyzes both <i>M. lysodeikticus</i> cell wall and <i>M. lysodeikticus</i> cell wall polysaccharide, while showing no activity against gelatin. The found peptide may be of interest for application in medicine, biotechnology, and the food industry, for example as a bio-preservative. |
| first_indexed | 2024-03-11T11:28:46Z |
| format | Article |
| id | doaj.art-7ede03fe05dc49a4ad740c9525916588 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-11T11:28:46Z |
| publishDate | 2023-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-7ede03fe05dc49a4ad740c95259165882023-11-10T15:04:36ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-10-0124211560710.3390/ijms242115607Novel Antimicrobial Peptide from the Hepatopancreas of the Red King CrabVladislav Molchanov0Alexander Yegorov1Maxim Molchanov2Alexander Timchenko3Vitaly Novikov4Nikolay Novojilov5Maria Timchenko6Institute of Theoretical and Experimental Biophysics RAS, Pushchino 142290, RussiaInstitute of Theoretical and Experimental Biophysics RAS, Pushchino 142290, RussiaInstitute of Theoretical and Experimental Biophysics RAS, Pushchino 142290, RussiaInstitute of Protein Research RAS, Pushchino 142290, RussiaPolar Branch of Russian Federal Research Institute of Fisheries and Oceanography, Murmansk 183038, RussiaM. M. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow 117997, RussiaInstitute of Theoretical and Experimental Biophysics RAS, Pushchino 142290, RussiaCrustaceans have successfully adapted to survive in their natural habitat, rich in microorganisms, due to the presence of antimicrobial peptides (AMPs) in their organism. They achieve this adaptation despite lacking the highly specific adaptive immune system found in vertebrates. One valuable source of AMPs is the hepatopancreas, a waste product from crab fishery and its processing. Applying zymographic and spectrophotometric techniques, we discovered a small peptide (approximately 5 kDa) within a low molecular weight protein fraction extracted from the acetone powder of the red king crab hepatopancreas. This peptide hydrolyzes both <i>M. lysodeikticus</i> cell wall and <i>M. lysodeikticus</i> cell wall polysaccharide, while showing no activity against gelatin. The found peptide may be of interest for application in medicine, biotechnology, and the food industry, for example as a bio-preservative.https://www.mdpi.com/1422-0067/24/21/15607antimicrobial peptideshepatopancreasred king crab |
| spellingShingle | Vladislav Molchanov Alexander Yegorov Maxim Molchanov Alexander Timchenko Vitaly Novikov Nikolay Novojilov Maria Timchenko Novel Antimicrobial Peptide from the Hepatopancreas of the Red King Crab International Journal of Molecular Sciences antimicrobial peptides hepatopancreas red king crab |
| title | Novel Antimicrobial Peptide from the Hepatopancreas of the Red King Crab |
| title_full | Novel Antimicrobial Peptide from the Hepatopancreas of the Red King Crab |
| title_fullStr | Novel Antimicrobial Peptide from the Hepatopancreas of the Red King Crab |
| title_full_unstemmed | Novel Antimicrobial Peptide from the Hepatopancreas of the Red King Crab |
| title_short | Novel Antimicrobial Peptide from the Hepatopancreas of the Red King Crab |
| title_sort | novel antimicrobial peptide from the hepatopancreas of the red king crab |
| topic | antimicrobial peptides hepatopancreas red king crab |
| url | https://www.mdpi.com/1422-0067/24/21/15607 |
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