Antioxidant, Antibacterial Properties of Novel Peptide CP by Enzymatic Hydrolysis of <i>Chromis notata</i> By-Products and Its Efficacy on Atopic Dermatitis
This study investigated the antioxidant, antimicrobial, and anti-atopic dermatitis (AD) effects of a novel peptide (CP) derived from a <i>Chromis notata</i> by-product hydrolysate. Alcalase, Flavourzyme, Neutrase, and Protamex enzymes were used to hydrolyze the <i>C. notata</i&g...
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| Format: | Article |
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MDPI AG
2024-01-01
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| Series: | Marine Drugs |
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| Online Access: | https://www.mdpi.com/1660-3397/22/1/44 |
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| author | Jin-Woo Hwang Sung-Gyu Lee Hyun Kang |
| author_facet | Jin-Woo Hwang Sung-Gyu Lee Hyun Kang |
| author_sort | Jin-Woo Hwang |
| collection | DOAJ |
| description | This study investigated the antioxidant, antimicrobial, and anti-atopic dermatitis (AD) effects of a novel peptide (CP) derived from a <i>Chromis notata</i> by-product hydrolysate. Alcalase, Flavourzyme, Neutrase, and Protamex enzymes were used to hydrolyze the <i>C. notata</i> by-product protein, and the 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical-scavenging activity was measured. Alcalase hydrolysate exhibited the highest ABTS radical-scavenging activity, leading to the selection of Alcalase for further purification. The CHAO-1-I fraction, with the highest ABTS activity, was isolated and further purified, resulting in the identification of the peptide CP with the amino acid sequence Ala-Gln-Val-Met-Lys-Leu-Pro-His-Arg-Met-Gln-His-Ser-Gln-Ser. CP demonstrated antimicrobial activity against <i>Staphylococcus aureus</i>, inhibiting its growth. In a 2,4-dinitrochlorobenzene (DNCB)-induced AD-like skin model in mice, CP significantly alleviated skin lesions, reduced epidermal and dermal thickness, and inhibited mast cell infiltration. Moreover, CP suppressed the elevated levels of interleukin-6 (IL-6) in the plasma of DNCB-induced mice. These findings highlight the potential of CP as a therapeutic agent for AD and suggest a novel application of this <i>C. notata</i> by-product in the fish processing industry. |
| first_indexed | 2024-03-08T10:43:26Z |
| format | Article |
| id | doaj.art-7edf939b5f2c4267903ae2d02e940b22 |
| institution | Directory Open Access Journal |
| issn | 1660-3397 |
| language | English |
| last_indexed | 2024-03-08T10:43:26Z |
| publishDate | 2024-01-01 |
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| record_format | Article |
| series | Marine Drugs |
| spelling | doaj.art-7edf939b5f2c4267903ae2d02e940b222024-01-26T17:26:16ZengMDPI AGMarine Drugs1660-33972024-01-012214410.3390/md22010044Antioxidant, Antibacterial Properties of Novel Peptide CP by Enzymatic Hydrolysis of <i>Chromis notata</i> By-Products and Its Efficacy on Atopic DermatitisJin-Woo Hwang0Sung-Gyu Lee1Hyun Kang2Department of Medical Laboratory Science, College of Health Science, Dankook University, Cheonan-si 31116, Chungcheongnam-do, Republic of KoreaDepartment of Medical Laboratory Science, College of Health Science, Dankook University, Cheonan-si 31116, Chungcheongnam-do, Republic of KoreaDepartment of Medical Laboratory Science, College of Health Science, Dankook University, Cheonan-si 31116, Chungcheongnam-do, Republic of KoreaThis study investigated the antioxidant, antimicrobial, and anti-atopic dermatitis (AD) effects of a novel peptide (CP) derived from a <i>Chromis notata</i> by-product hydrolysate. Alcalase, Flavourzyme, Neutrase, and Protamex enzymes were used to hydrolyze the <i>C. notata</i> by-product protein, and the 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical-scavenging activity was measured. Alcalase hydrolysate exhibited the highest ABTS radical-scavenging activity, leading to the selection of Alcalase for further purification. The CHAO-1-I fraction, with the highest ABTS activity, was isolated and further purified, resulting in the identification of the peptide CP with the amino acid sequence Ala-Gln-Val-Met-Lys-Leu-Pro-His-Arg-Met-Gln-His-Ser-Gln-Ser. CP demonstrated antimicrobial activity against <i>Staphylococcus aureus</i>, inhibiting its growth. In a 2,4-dinitrochlorobenzene (DNCB)-induced AD-like skin model in mice, CP significantly alleviated skin lesions, reduced epidermal and dermal thickness, and inhibited mast cell infiltration. Moreover, CP suppressed the elevated levels of interleukin-6 (IL-6) in the plasma of DNCB-induced mice. These findings highlight the potential of CP as a therapeutic agent for AD and suggest a novel application of this <i>C. notata</i> by-product in the fish processing industry.https://www.mdpi.com/1660-3397/22/1/44<i>Chromis notata</i>by-product hydrolysateantioxidant peptideantimicrobial activityatopic dermatitis |
| spellingShingle | Jin-Woo Hwang Sung-Gyu Lee Hyun Kang Antioxidant, Antibacterial Properties of Novel Peptide CP by Enzymatic Hydrolysis of <i>Chromis notata</i> By-Products and Its Efficacy on Atopic Dermatitis Marine Drugs <i>Chromis notata</i> by-product hydrolysate antioxidant peptide antimicrobial activity atopic dermatitis |
| title | Antioxidant, Antibacterial Properties of Novel Peptide CP by Enzymatic Hydrolysis of <i>Chromis notata</i> By-Products and Its Efficacy on Atopic Dermatitis |
| title_full | Antioxidant, Antibacterial Properties of Novel Peptide CP by Enzymatic Hydrolysis of <i>Chromis notata</i> By-Products and Its Efficacy on Atopic Dermatitis |
| title_fullStr | Antioxidant, Antibacterial Properties of Novel Peptide CP by Enzymatic Hydrolysis of <i>Chromis notata</i> By-Products and Its Efficacy on Atopic Dermatitis |
| title_full_unstemmed | Antioxidant, Antibacterial Properties of Novel Peptide CP by Enzymatic Hydrolysis of <i>Chromis notata</i> By-Products and Its Efficacy on Atopic Dermatitis |
| title_short | Antioxidant, Antibacterial Properties of Novel Peptide CP by Enzymatic Hydrolysis of <i>Chromis notata</i> By-Products and Its Efficacy on Atopic Dermatitis |
| title_sort | antioxidant antibacterial properties of novel peptide cp by enzymatic hydrolysis of i chromis notata i by products and its efficacy on atopic dermatitis |
| topic | <i>Chromis notata</i> by-product hydrolysate antioxidant peptide antimicrobial activity atopic dermatitis |
| url | https://www.mdpi.com/1660-3397/22/1/44 |
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