Humulus japonicus attenuates LPS-and scopolamine-induced cognitive impairment in mice
Abstract Background Neuroinflammation plays an important role in cognitive decline and memory impairment in neurodegenerative disorders. Previously, we demonstrated that Humulus japonicus (HJ) has anti-inflammatory effects in rodent models of Alzheimer’s disease and Parkinson’s disease. The present...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2022-07-01
|
| Series: | Laboratory Animal Research |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s42826-022-00134-3 |
| _version_ | 1818192897218969600 |
|---|---|
| author | Jun Go Hye-Yeon Park Da Woon Lee So-Young Maeng In-Bok Lee Yun Jeong Seo Jin-Pyo An Won Keun Oh Chul-Ho Lee Kyoung-Shim Kim |
| author_facet | Jun Go Hye-Yeon Park Da Woon Lee So-Young Maeng In-Bok Lee Yun Jeong Seo Jin-Pyo An Won Keun Oh Chul-Ho Lee Kyoung-Shim Kim |
| author_sort | Jun Go |
| collection | DOAJ |
| description | Abstract Background Neuroinflammation plays an important role in cognitive decline and memory impairment in neurodegenerative disorders. Previously, we demonstrated that Humulus japonicus (HJ) has anti-inflammatory effects in rodent models of Alzheimer’s disease and Parkinson’s disease. The present study aimed to examine the protective potential of HJ extracts against lipopolysaccharide (LPS)-induced cognitive impairment and scopolamine-induced amnesia in mouse models. Cognitive improvement of mice was investigated by novel object recognition test. For analyzing effects on neuroinflammation, immunohistochemistry and quantitative real-time polymerase chain reaction (qRT-PCR) assays were performed. Results We found that the oral administration of HJ significantly improved cognitive dysfunction induced by LPS in a novel object recognition test. The LPS-induced activation of microglia was notably decreased by HJ treatment in the cortex and hippocampus. HJ administration with LPS also significantly increased the mRNA expression of interleukin (IL)-10 and decreased the mRNA expression of IL-12 in the parietal cortex of mice. The increased expression of LPS-induced complement C1q B chain (C1bq) and triggering receptor expressed on myeloid cells 2 (Trem2) genes was significantly suppressed by HJ treatment. In addition, HJ administration significantly improved novel object recognition in a scopolamine-induced amnesia mouse model. Conclusions These findings revealed that HJ has a beneficial effect on cognitive impairment and neuroinflammation induced by systemic inflammation and on amnesia induced by scopolamine in mice. |
| first_indexed | 2024-12-12T00:37:48Z |
| format | Article |
| id | doaj.art-7ee15ed5b28c46328eab651c6c2f2bda |
| institution | Directory Open Access Journal |
| issn | 2233-7660 |
| language | English |
| last_indexed | 2024-12-12T00:37:48Z |
| publishDate | 2022-07-01 |
| publisher | BMC |
| record_format | Article |
| series | Laboratory Animal Research |
| spelling | doaj.art-7ee15ed5b28c46328eab651c6c2f2bda2022-12-22T00:44:19ZengBMCLaboratory Animal Research2233-76602022-07-0138111010.1186/s42826-022-00134-3Humulus japonicus attenuates LPS-and scopolamine-induced cognitive impairment in miceJun Go0Hye-Yeon Park1Da Woon Lee2So-Young Maeng3In-Bok Lee4Yun Jeong Seo5Jin-Pyo An6Won Keun Oh7Chul-Ho Lee8Kyoung-Shim Kim9Laboratory Animal Resource Center, Korea Research Institute of Bioscience and BiotechnologyLaboratory Animal Resource Center, Korea Research Institute of Bioscience and BiotechnologyLaboratory Animal Resource Center, Korea Research Institute of Bioscience and BiotechnologyLaboratory Animal Resource Center, Korea Research Institute of Bioscience and BiotechnologyLaboratory Animal Resource Center, Korea Research Institute of Bioscience and BiotechnologyLaboratory Animal Resource Center, Korea Research Institute of Bioscience and BiotechnologyKorea Bioactive Natural Material Bank, Research Institute of Pharmaceutical Sciences College of Pharmacy, Seoul National University (SNU)Korea Bioactive Natural Material Bank, Research Institute of Pharmaceutical Sciences College of Pharmacy, Seoul National University (SNU)Laboratory Animal Resource Center, Korea Research Institute of Bioscience and BiotechnologyLaboratory Animal Resource Center, Korea Research Institute of Bioscience and BiotechnologyAbstract Background Neuroinflammation plays an important role in cognitive decline and memory impairment in neurodegenerative disorders. Previously, we demonstrated that Humulus japonicus (HJ) has anti-inflammatory effects in rodent models of Alzheimer’s disease and Parkinson’s disease. The present study aimed to examine the protective potential of HJ extracts against lipopolysaccharide (LPS)-induced cognitive impairment and scopolamine-induced amnesia in mouse models. Cognitive improvement of mice was investigated by novel object recognition test. For analyzing effects on neuroinflammation, immunohistochemistry and quantitative real-time polymerase chain reaction (qRT-PCR) assays were performed. Results We found that the oral administration of HJ significantly improved cognitive dysfunction induced by LPS in a novel object recognition test. The LPS-induced activation of microglia was notably decreased by HJ treatment in the cortex and hippocampus. HJ administration with LPS also significantly increased the mRNA expression of interleukin (IL)-10 and decreased the mRNA expression of IL-12 in the parietal cortex of mice. The increased expression of LPS-induced complement C1q B chain (C1bq) and triggering receptor expressed on myeloid cells 2 (Trem2) genes was significantly suppressed by HJ treatment. In addition, HJ administration significantly improved novel object recognition in a scopolamine-induced amnesia mouse model. Conclusions These findings revealed that HJ has a beneficial effect on cognitive impairment and neuroinflammation induced by systemic inflammation and on amnesia induced by scopolamine in mice.https://doi.org/10.1186/s42826-022-00134-3Humulus japonicasCognitive functionNeuroinflammationMouse model |
| spellingShingle | Jun Go Hye-Yeon Park Da Woon Lee So-Young Maeng In-Bok Lee Yun Jeong Seo Jin-Pyo An Won Keun Oh Chul-Ho Lee Kyoung-Shim Kim Humulus japonicus attenuates LPS-and scopolamine-induced cognitive impairment in mice Laboratory Animal Research Humulus japonicas Cognitive function Neuroinflammation Mouse model |
| title | Humulus japonicus attenuates LPS-and scopolamine-induced cognitive impairment in mice |
| title_full | Humulus japonicus attenuates LPS-and scopolamine-induced cognitive impairment in mice |
| title_fullStr | Humulus japonicus attenuates LPS-and scopolamine-induced cognitive impairment in mice |
| title_full_unstemmed | Humulus japonicus attenuates LPS-and scopolamine-induced cognitive impairment in mice |
| title_short | Humulus japonicus attenuates LPS-and scopolamine-induced cognitive impairment in mice |
| title_sort | humulus japonicus attenuates lps and scopolamine induced cognitive impairment in mice |
| topic | Humulus japonicas Cognitive function Neuroinflammation Mouse model |
| url | https://doi.org/10.1186/s42826-022-00134-3 |
| work_keys_str_mv | AT jungo humulusjaponicusattenuateslpsandscopolamineinducedcognitiveimpairmentinmice AT hyeyeonpark humulusjaponicusattenuateslpsandscopolamineinducedcognitiveimpairmentinmice AT dawoonlee humulusjaponicusattenuateslpsandscopolamineinducedcognitiveimpairmentinmice AT soyoungmaeng humulusjaponicusattenuateslpsandscopolamineinducedcognitiveimpairmentinmice AT inboklee humulusjaponicusattenuateslpsandscopolamineinducedcognitiveimpairmentinmice AT yunjeongseo humulusjaponicusattenuateslpsandscopolamineinducedcognitiveimpairmentinmice AT jinpyoan humulusjaponicusattenuateslpsandscopolamineinducedcognitiveimpairmentinmice AT wonkeunoh humulusjaponicusattenuateslpsandscopolamineinducedcognitiveimpairmentinmice AT chulholee humulusjaponicusattenuateslpsandscopolamineinducedcognitiveimpairmentinmice AT kyoungshimkim humulusjaponicusattenuateslpsandscopolamineinducedcognitiveimpairmentinmice |